Leucomyxa plasmidifera gen.

IF 4.5 3区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Open Biology Pub Date : 2024-10-01 Epub Date: 2024-10-30 DOI:10.1098/rsob.240022
Dovilė Barcytė, Karin Jaške, Tomáš Pánek, Tatiana Yurchenko, Tereza Ševčíková, Anežka Eliášová, Marek Eliáš
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引用次数: 0

摘要

在第二类非光合真核生物中,完全丧失质体的情况似乎非常罕见。根据之前的透射电子显微镜研究,Leukarachnion sp. PRA-24(一种与光合藻类同步叶绿藻(Ochrophyta)有关的无色变形原生生物)是这种情况的候选者。在这里,我们进一步详细描述了这种生物的特征,并将其描述为 Leucomyxa plasmidifera gen.我们恢复了其完整的质体基因组,发现其质体基因组与其他非光合水华藻的质体基因组相似,但在序列差异方面却更为极端。在L. plasmidifera转录组中鉴定出了质体蛋白质导入机制的组成部分,这证实了该生物拥有一个隐蔽的质体细胞器。根据我们的生物信息学重建,该质体包含一个生产血红素、叶酸前体和生育三烯酚的独特生物合成途径组合。作为其细胞器生物学的另一个转折,L. plasmidifera 的有丝分裂基因组中含有一个不寻常的长插入物,该插入物与新发现的线粒体质粒有关,在大小和编码能力方面表现出前所未有的特征。综上所述,我们的工作进一步揭示了半自主细胞器在原生生物进化过程中的惊人结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A cryptic plastid and a novel mitochondrial plasmid in Leucomyxa plasmidifera gen. and sp. nov. (Ochrophyta) push the frontiers of organellar biology.

Complete plastid loss seems to be very rare among secondarily non-photosynthetic eukaryotes. Leukarachnion sp. PRA-24, an amoeboid colourless protist related to the photosynthetic algal class Synchromophyceae (Ochrophyta), is a candidate for such a case based on a previous investigation by transmission electron microscopy. Here, we characterize this organism in further detail and describe it as Leucomyxa plasmidifera gen. et sp. nov., additionally demonstrating it is the first known representative of a broader clade of non-photosynthetic ochrophytes. We recovered its complete plastid genome, exhibiting a reduced gene set similar to plastomes of other non-photosynthetic ochrophytes, yet being even more extreme in sequence divergence. Identification of components of the plastid protein import machinery in the L. plasmidifera transcriptome assembly corroborated that the organism possesses a cryptic plastid organelle. According to our bioinformatic reconstruction, the plastid contains a unique combination of biosynthetic pathways producing haem, a folate precursor and tocotrienols. As another twist to its organellar biology, L. plasmidifera turned out to contain an unusual long insertion in its mitogenome related to a newly discovered mitochondrial plasmid exhibiting unprecedented features in terms of its size and coding capacity. Combined, our work uncovered further striking outcomes of the evolutionary course of semiautonomous organelles in protists.

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来源期刊
Open Biology
Open Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
10.00
自引率
1.70%
发文量
136
审稿时长
6-12 weeks
期刊介绍: Open Biology is an online journal that welcomes original, high impact research in cell and developmental biology, molecular and structural biology, biochemistry, neuroscience, immunology, microbiology and genetics.
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