{"title":"产犊前注射微量矿物质补充剂对季节性产犊牧区奶牛白细胞功能的影响。","authors":"A J Bates, M Wells, C Fitzpatrick, R A Laven","doi":"10.1080/00480169.2024.2417925","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>To investigate the effect of injection of trace mineral supplement (TMS) 14-28 days before calving on white blood cell count (WBCC) and function, serum antioxidant capacity (SAC) and reactive oxygen species (ROS) in pasture-fed cattle after calving.</p><p><strong>Methods: </strong>On each of two South Island, seasonally calving, pastoral dairy farms,1 month before dry-off, a random sample of 150 multiparous cows predicted to calve within 7 days of the herd's planned start of calving (PSC) were stratified on individual somatic cell count, age, breed and expected calving date. On each farm, 14-24 days before PSC, 60 selected cows were randomly assigned for TMS (Zn, Mn, Se, Cu) injection, and 60 were controls. All 240 cows were contemporaneously injected with hydroxocobalamin, and controls with Se. Blood samples were collected pre-injection and 3, 12 and 40 days after calving. Phagocytic activity, count and proportion of neutrophils, lymphocytes and monocytes, WBCC, ROS, SAC were measured. Plasma concentrations of Se, Cu and glutathione peroxidase (GPx) were monitored from a random subset of animals. Differences attributable to TMS were estimated using mixed-multivariable Bayesian analysis, expressed as mean and highest density interval (HDI).</p><p><strong>Results: </strong>Three and 40 days after calving, TMS-treated cows had 0.36 (90% HDI = 0.00-0.77) x 10<sup>9</sup> and 0.25 (90% HDI = 0.00-0.55) x 10<sup>9</sup> fewer neutrophils/L. Neutrophils comprised 6 (90% HDI = 0-11)% and 4 (90% HDI = 0-8)% less of the WBCC, and the neutrophil count was 14 (90% HDI = 0-27)% and 9 (90% HDI = 0-18)% less than controls. However, 3 days after calving, there were 7 (95% HDI = 2-12)% more cells phagocytosing and 2,900 (95% HDI = 2,600-3,200) more bacteria ingested/cell. Twelve and 40 days after calving, TMS-treated cows had 0.65 (95% HDI = 0.17-1.17) x 10<sup>9</sup> and 0.28 (95% HDI = 0.00-0.59) x 10<sup>9</sup> more lymphocytes/L. Lymphocytes comprised 10 (95% HDI = 3-18)% and 5 (95% HDI = 0-9)% more of the WBCC, and the lymphocyte count was 30 (95% HDI = 11-51)% and 9 (95% HDI = 0-9)% more than controls. There were no meaningful differences in ROS, SAC, ROS/SAC, other white blood cells, or WBCC. Plasma Cu, Se and GPx concentrations were above recommended thresholds.</p><p><strong>Conclusions: </strong>Pre-calving TMS injection was associated with differences in white blood cell population and function that may reduce the risk of disease.</p><p><strong>Abbreviations: </strong>BHOB: Beta-hydroxybutyrate; GPx: Glutathione peroxidase; HDI: Highest density interval; MESF: Molecules of equivalent soluble fluorophore; OSi: Oxidative stress index; PSC: Planned start of calving; ROPE: Region of probable equivalence; ROS: Reactive oxygen species; SAC: Serum antioxidant capacity; THI: Temperature humidity index; TMS: Trace mineral supplement; WAIC: Widely applicable information criterion; WBCC: White blood cell count.</p>","PeriodicalId":19322,"journal":{"name":"New Zealand veterinary journal","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of a pre-calving injectable trace mineral supplement on white blood cell function in seasonally calving pastoral dairy cows.\",\"authors\":\"A J Bates, M Wells, C Fitzpatrick, R A Laven\",\"doi\":\"10.1080/00480169.2024.2417925\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>To investigate the effect of injection of trace mineral supplement (TMS) 14-28 days before calving on white blood cell count (WBCC) and function, serum antioxidant capacity (SAC) and reactive oxygen species (ROS) in pasture-fed cattle after calving.</p><p><strong>Methods: </strong>On each of two South Island, seasonally calving, pastoral dairy farms,1 month before dry-off, a random sample of 150 multiparous cows predicted to calve within 7 days of the herd's planned start of calving (PSC) were stratified on individual somatic cell count, age, breed and expected calving date. On each farm, 14-24 days before PSC, 60 selected cows were randomly assigned for TMS (Zn, Mn, Se, Cu) injection, and 60 were controls. All 240 cows were contemporaneously injected with hydroxocobalamin, and controls with Se. Blood samples were collected pre-injection and 3, 12 and 40 days after calving. Phagocytic activity, count and proportion of neutrophils, lymphocytes and monocytes, WBCC, ROS, SAC were measured. Plasma concentrations of Se, Cu and glutathione peroxidase (GPx) were monitored from a random subset of animals. Differences attributable to TMS were estimated using mixed-multivariable Bayesian analysis, expressed as mean and highest density interval (HDI).</p><p><strong>Results: </strong>Three and 40 days after calving, TMS-treated cows had 0.36 (90% HDI = 0.00-0.77) x 10<sup>9</sup> and 0.25 (90% HDI = 0.00-0.55) x 10<sup>9</sup> fewer neutrophils/L. Neutrophils comprised 6 (90% HDI = 0-11)% and 4 (90% HDI = 0-8)% less of the WBCC, and the neutrophil count was 14 (90% HDI = 0-27)% and 9 (90% HDI = 0-18)% less than controls. However, 3 days after calving, there were 7 (95% HDI = 2-12)% more cells phagocytosing and 2,900 (95% HDI = 2,600-3,200) more bacteria ingested/cell. Twelve and 40 days after calving, TMS-treated cows had 0.65 (95% HDI = 0.17-1.17) x 10<sup>9</sup> and 0.28 (95% HDI = 0.00-0.59) x 10<sup>9</sup> more lymphocytes/L. Lymphocytes comprised 10 (95% HDI = 3-18)% and 5 (95% HDI = 0-9)% more of the WBCC, and the lymphocyte count was 30 (95% HDI = 11-51)% and 9 (95% HDI = 0-9)% more than controls. There were no meaningful differences in ROS, SAC, ROS/SAC, other white blood cells, or WBCC. Plasma Cu, Se and GPx concentrations were above recommended thresholds.</p><p><strong>Conclusions: </strong>Pre-calving TMS injection was associated with differences in white blood cell population and function that may reduce the risk of disease.</p><p><strong>Abbreviations: </strong>BHOB: Beta-hydroxybutyrate; GPx: Glutathione peroxidase; HDI: Highest density interval; MESF: Molecules of equivalent soluble fluorophore; OSi: Oxidative stress index; PSC: Planned start of calving; ROPE: Region of probable equivalence; ROS: Reactive oxygen species; SAC: Serum antioxidant capacity; THI: Temperature humidity index; TMS: Trace mineral supplement; WAIC: Widely applicable information criterion; WBCC: White blood cell count.</p>\",\"PeriodicalId\":19322,\"journal\":{\"name\":\"New Zealand veterinary journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-10-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"New Zealand veterinary journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1080/00480169.2024.2417925\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Zealand veterinary journal","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1080/00480169.2024.2417925","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Effect of a pre-calving injectable trace mineral supplement on white blood cell function in seasonally calving pastoral dairy cows.
Aims: To investigate the effect of injection of trace mineral supplement (TMS) 14-28 days before calving on white blood cell count (WBCC) and function, serum antioxidant capacity (SAC) and reactive oxygen species (ROS) in pasture-fed cattle after calving.
Methods: On each of two South Island, seasonally calving, pastoral dairy farms,1 month before dry-off, a random sample of 150 multiparous cows predicted to calve within 7 days of the herd's planned start of calving (PSC) were stratified on individual somatic cell count, age, breed and expected calving date. On each farm, 14-24 days before PSC, 60 selected cows were randomly assigned for TMS (Zn, Mn, Se, Cu) injection, and 60 were controls. All 240 cows were contemporaneously injected with hydroxocobalamin, and controls with Se. Blood samples were collected pre-injection and 3, 12 and 40 days after calving. Phagocytic activity, count and proportion of neutrophils, lymphocytes and monocytes, WBCC, ROS, SAC were measured. Plasma concentrations of Se, Cu and glutathione peroxidase (GPx) were monitored from a random subset of animals. Differences attributable to TMS were estimated using mixed-multivariable Bayesian analysis, expressed as mean and highest density interval (HDI).
Results: Three and 40 days after calving, TMS-treated cows had 0.36 (90% HDI = 0.00-0.77) x 109 and 0.25 (90% HDI = 0.00-0.55) x 109 fewer neutrophils/L. Neutrophils comprised 6 (90% HDI = 0-11)% and 4 (90% HDI = 0-8)% less of the WBCC, and the neutrophil count was 14 (90% HDI = 0-27)% and 9 (90% HDI = 0-18)% less than controls. However, 3 days after calving, there were 7 (95% HDI = 2-12)% more cells phagocytosing and 2,900 (95% HDI = 2,600-3,200) more bacteria ingested/cell. Twelve and 40 days after calving, TMS-treated cows had 0.65 (95% HDI = 0.17-1.17) x 109 and 0.28 (95% HDI = 0.00-0.59) x 109 more lymphocytes/L. Lymphocytes comprised 10 (95% HDI = 3-18)% and 5 (95% HDI = 0-9)% more of the WBCC, and the lymphocyte count was 30 (95% HDI = 11-51)% and 9 (95% HDI = 0-9)% more than controls. There were no meaningful differences in ROS, SAC, ROS/SAC, other white blood cells, or WBCC. Plasma Cu, Se and GPx concentrations were above recommended thresholds.
Conclusions: Pre-calving TMS injection was associated with differences in white blood cell population and function that may reduce the risk of disease.
Abbreviations: BHOB: Beta-hydroxybutyrate; GPx: Glutathione peroxidase; HDI: Highest density interval; MESF: Molecules of equivalent soluble fluorophore; OSi: Oxidative stress index; PSC: Planned start of calving; ROPE: Region of probable equivalence; ROS: Reactive oxygen species; SAC: Serum antioxidant capacity; THI: Temperature humidity index; TMS: Trace mineral supplement; WAIC: Widely applicable information criterion; WBCC: White blood cell count.
期刊介绍:
The New Zealand Veterinary Journal (NZVJ) is an international journal publishing high quality peer-reviewed articles covering all aspects of veterinary science, including clinical practice, animal welfare and animal health.
The NZVJ publishes original research findings, clinical communications (including novel case reports and case series), rapid communications, correspondence and review articles, originating from New Zealand and internationally.
Topics should be relevant to, but not limited to, New Zealand veterinary and animal science communities, and include the disciplines of infectious disease, medicine, surgery and the health, management and welfare of production and companion animals, horses and New Zealand wildlife.
All submissions are expected to meet the highest ethical and welfare standards, as detailed in the Journal’s instructions for authors.