产犊前注射微量矿物质补充剂对季节性产犊牧区奶牛白细胞功能的影响。

IF 1.1 4区 农林科学 Q3 VETERINARY SCIENCES
A J Bates, M Wells, C Fitzpatrick, R A Laven
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引用次数: 0

摘要

目的:研究在产犊前 14-28 天注射微量矿物质补充剂(TMS)对产犊后牧草喂养牛的白细胞计数(WBCC)和功能、血清抗氧化能力(SAC)和活性氧(ROS)的影响:在南岛两个季节性产犊的牧区奶牛场,在干草枯竭前 1 个月,根据个体体细胞数、年龄、品种和预期产犊日期,对预测将在牛群计划产犊期(PSC)前 7 天内产犊的 150 头多胎奶牛进行分层随机抽样。每个牧场在计划产犊前 14-24 天随机挑选 60 头奶牛注射 TMS(锌、锰、硒、铜),60 头为对照组。所有 240 头奶牛同时注射羟钴胺,对照组注射 Se。在注射前以及产犊后 3 天、12 天和 40 天采集血液样本。测量吞噬活性、中性粒细胞、淋巴细胞和单核细胞的数量和比例、WBCC、ROS、SAC。随机抽取一部分动物监测血浆中的硒、铜和谷胱甘肽过氧化物酶(GPx)浓度。采用混合多变量贝叶斯分析法估算了可归因于TMS的差异,以平均值和最高密度间隔(HDI)表示:产犊后3天和40天,经TMS处理的奶牛中性粒细胞/升分别减少0.36(90% HDI = 0.00-0.77)×109和0.25(90% HDI = 0.00-0.55)×109。与对照组相比,中性粒细胞占WBCC的比例分别减少了6(90% HDI = 0-11)%和4(90% HDI = 0-8)%,中性粒细胞计数分别减少了14(90% HDI = 0-27)%和9(90% HDI = 0-18)%。然而,产犊 3 天后,吞噬细胞增加了 7 (95% HDI = 2-12)%,摄入的细菌/细胞增加了 2,900 (95% HDI = 2,600-3,200) 个。产犊后 12 天和 40 天,经 TMS 处理的奶牛的淋巴细胞/升分别增加 0.65(95% HDI = 0.17-1.17)×109 和 0.28(95% HDI = 0.00-0.59)×109。淋巴细胞占 WBCC 的 10 (95% HDI = 3-18)% 和 5 (95% HDI = 0-9)% 以上,淋巴细胞计数比对照组多 30 (95% HDI = 11-51)% 和 9 (95% HDI = 0-9)%。ROS、SAC、ROS/SAC、其他白细胞或 WBCC 均无明显差异。血浆中Cu、Se和GPx的浓度高于建议的阈值:结论:犊牛产前注射 TMS 与白细胞数量和功能的差异有关,可降低疾病风险:缩写:BHOB:β-羟基丁酸盐;GPx:谷胱甘肽过氧化物酶;BHOB:β-羟基丁酸盐:缩写:BHOB:β-羟基丁酸;GPx:谷胱甘肽过氧化物酶;HDI:最高密度间隔;MESF:等效可溶性荧光团分子;OSi:氧化应激指数;PSC:计划产犊开始时间;ROPE:可能等效区域;ROS:氧化应激指数:ROS:反应性氧物种;SAC:血清素:SAC:血清抗氧化能力;THI:温度湿度指数;TMS:微量元素补充剂;WAIC:广泛适用的信息标准;WBCC:白细胞计数:白细胞计数。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of a pre-calving injectable trace mineral supplement on white blood cell function in seasonally calving pastoral dairy cows.

Aims: To investigate the effect of injection of trace mineral supplement (TMS) 14-28 days before calving on white blood cell count (WBCC) and function, serum antioxidant capacity (SAC) and reactive oxygen species (ROS) in pasture-fed cattle after calving.

Methods: On each of two South Island, seasonally calving, pastoral dairy farms,1 month before dry-off, a random sample of 150 multiparous cows predicted to calve within 7 days of the herd's planned start of calving (PSC) were stratified on individual somatic cell count, age, breed and expected calving date. On each farm, 14-24 days before PSC, 60 selected cows were randomly assigned for TMS (Zn, Mn, Se, Cu) injection, and 60 were controls. All 240 cows were contemporaneously injected with hydroxocobalamin, and controls with Se. Blood samples were collected pre-injection and 3, 12 and 40 days after calving. Phagocytic activity, count and proportion of neutrophils, lymphocytes and monocytes, WBCC, ROS, SAC were measured. Plasma concentrations of Se, Cu and glutathione peroxidase (GPx) were monitored from a random subset of animals. Differences attributable to TMS were estimated using mixed-multivariable Bayesian analysis, expressed as mean and highest density interval (HDI).

Results: Three and 40 days after calving, TMS-treated cows had 0.36 (90% HDI = 0.00-0.77) x 109 and 0.25 (90% HDI = 0.00-0.55) x 109 fewer neutrophils/L. Neutrophils comprised 6 (90% HDI = 0-11)% and 4 (90% HDI = 0-8)% less of the WBCC, and the neutrophil count was 14 (90% HDI = 0-27)% and 9 (90% HDI = 0-18)% less than controls. However, 3 days after calving, there were 7 (95% HDI = 2-12)% more cells phagocytosing and 2,900 (95% HDI = 2,600-3,200) more bacteria ingested/cell. Twelve and 40 days after calving, TMS-treated cows had 0.65 (95% HDI = 0.17-1.17) x 109 and 0.28 (95% HDI = 0.00-0.59) x 109 more lymphocytes/L. Lymphocytes comprised 10 (95% HDI = 3-18)% and 5 (95% HDI = 0-9)% more of the WBCC, and the lymphocyte count was 30 (95% HDI = 11-51)% and 9 (95% HDI = 0-9)% more than controls. There were no meaningful differences in ROS, SAC, ROS/SAC, other white blood cells, or WBCC. Plasma Cu, Se and GPx concentrations were above recommended thresholds.

Conclusions: Pre-calving TMS injection was associated with differences in white blood cell population and function that may reduce the risk of disease.

Abbreviations: BHOB: Beta-hydroxybutyrate; GPx: Glutathione peroxidase; HDI: Highest density interval; MESF: Molecules of equivalent soluble fluorophore; OSi: Oxidative stress index; PSC: Planned start of calving; ROPE: Region of probable equivalence; ROS: Reactive oxygen species; SAC: Serum antioxidant capacity; THI: Temperature humidity index; TMS: Trace mineral supplement; WAIC: Widely applicable information criterion; WBCC: White blood cell count.

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来源期刊
New Zealand veterinary journal
New Zealand veterinary journal 农林科学-兽医学
CiteScore
3.00
自引率
0.00%
发文量
37
审稿时长
12-24 weeks
期刊介绍: The New Zealand Veterinary Journal (NZVJ) is an international journal publishing high quality peer-reviewed articles covering all aspects of veterinary science, including clinical practice, animal welfare and animal health. The NZVJ publishes original research findings, clinical communications (including novel case reports and case series), rapid communications, correspondence and review articles, originating from New Zealand and internationally. Topics should be relevant to, but not limited to, New Zealand veterinary and animal science communities, and include the disciplines of infectious disease, medicine, surgery and the health, management and welfare of production and companion animals, horses and New Zealand wildlife. All submissions are expected to meet the highest ethical and welfare standards, as detailed in the Journal’s instructions for authors.
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