RT-qPCR 简要指南。

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2024-10-28 DOI:10.1016/j.mocell.2024.100141

Dajeong Bong, Jooyeon Sohn, Seung-Jae V. Lee

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引用次数: 0

摘要

RNA 定量对于了解基因表达和调控至关重要。反转录-定量聚合酶链反应（RT-qPCR）因其实用性、定量性、灵敏性和特异性而被广泛应用于 RNA 定量。在此，我们将对 RT-qPCR 进行概述，重点介绍基本试剂、引物设计的重要性、详细的工作流程和数据分析方法。本指南将为不熟悉 RT-qPCR 的科学家提供帮助，重点介绍成功进行 RNA 定量的关键注意事项。

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Brief guide to RT-qPCR

RNA quantification is crucial for understanding gene expression and regulation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a widely used technique for RNA quantification because of its practical and quantitative nature, sensitivity, and specificity. Here, we provide an overview of RT-qPCR, focusing on essential reagents, the importance of primer design, the detailed workflow, and data analysis methods. This guide will be useful for scientists who are unfamiliar with RT-qPCR, highlighting key considerations for successful RNA quantification.

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is "Mol. Cells". Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.