三重免疫染色显示人类原发性骨髓纤维化骨髓中可能存在含分离核的非典型单核细胞:一份病例报告。

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL
Shunsuke Homma, Toshie Ogasawara, Michie Suga, Yoshiyasu Nakamura, Katsuya Takenaka, Shoko Marshall, Kiyotaka Kawauchi, Naoki Mori, Hajime Kuroda, Naoya Nakamura, Yohei Miyagi, Atsuko Masunaga
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引用次数: 0

摘要

背景:最近在小鼠体内发现了含分离核的非典型单核细胞。含分离核的非典型单核细胞被认为起源于骨髓,并诱导药物损伤肺的纤维化。Lyc6c-小鼠单核细胞亚群与人类 CD14-CD16++ 非典型单核细胞相对应;然而,与小鼠含分离核非典型单核细胞相对应的人类单核细胞尚未发现。原发性骨髓纤维化是一种众所周知的进行性骨髓纤维化疾病,非典型巨核细胞被认为与原发性骨髓纤维化骨髓纤维化密切相关。然而,最近有报道称,单核细胞在原发性骨髓纤维化的骨髓纤维化中起着重要作用。我们推测,如果存在与小鼠含分离核的非典型单核细胞相对应的人类单核细胞,那么它将呈现与小鼠含分离核的非典型单核细胞相同的标记,如CD14-CD16+巨噬细胞-1抗原(CD11b/CD18复合物)+、MSR1+和CEACAM1+,而且它可能存在于原发性骨髓纤维化患者的骨髓中:一名 74 岁的日本男性因前列腺癌行全前列腺切除术后到我院进行临床随访。在定期检查中突然发现贫血、血小板减少和乳酸脱氢酶升高。经检查发现,该患者存在 CALR 2 型突变(p.K385fs*47)。患者骨髓的组织学特征与纤维化原发性骨髓纤维化一致。我们对骨髓进行了免疫组化研究,试图找出与鼠类含分离核的非典型单核细胞相对应的人类单核细胞。通过三重免疫染色,我们检测到少量 CD16+MSR1+CEACAM1+ 细胞,但没有 CD14+MSR1+CEACAM1+ 细胞。患者目前状况良好,无需接受原发性骨髓纤维化治疗:结论:原发性骨髓纤维化患者骨髓中可能存在含人类分离核的非典型单核细胞,并可能与骨髓纤维化有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Triple immunostaining demonstrates the possible existence of segregated-nucleus-containing atypical monocytes in human primary myelofibrosis bone marrow: a case report.

Background: Segregated-nucleus-containing atypical monocytes have recently been identified in mice. Segregated-nucleus-containing atypical monocytes are thought to originate from the bone marrow and induce fibrosis in the drug-injured lung. The Lyc6c- murine monocyte subset is the counterpart to human CD14-CD16++ non-classical monocytes; however, the human counterpart to murine segregated-nucleus-containing atypical monocytes has not yet been identified. Primary myelofibrosis is a well-known disease of progressive marrow fibrosis, and atypical megakaryocytes are thought to be closely related to fibrosis in primary myelofibrosis bone marrow. However, recently, monocytes have been reported to play an important role in marrow fibrosis in primary myelofibrosis. We speculated that, if there is a human counterpart to murine segregated-nucleus-containing atypical monocytes, it would present the same markers as murine segregated-nucleus-containing atypical monocytes, such as CD14-CD16+ macrophage-1 antigen (CD11b/CD18 complex)+, MSR1+, and CEACAM1+, and it might exist in the bone marrow of patients with primary myelofibrosis.

Case presentation: A 74-year-old Japanese male visited our hospital for clinical follow-up after total prostatectomy for prostatic cancer. Anemia, thrombocytosis, and elevated lactate dehydrogenase were suddenly observed in a periodic examination. CALR mutation type 2 (p.K385fs*47) was observed. The histological features of the patient's bone marrow were consistent with fibrotic primary myelofibrosis. We immunohistochemically studied the bone marrow in an attempt to identify a human counterpart to murine segregated-nucleus-containing atypical monocytes. We detected a few CD16+MSR1+CEACAM1+ cells, but not CD14+MSR1+CEACAM1+ cells, by triple immunostaining. The patient is in a good condition and does not require treatment for primary myelofibrosis.

Conclusion: There is a possibility that human segregated-nucleus-containing atypical monocytes exist in the bone marrow of primary myelofibrosis patients and might be related to marrow fibrosis.

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来源期刊
Journal of Medical Case Reports
Journal of Medical Case Reports Medicine-Medicine (all)
CiteScore
1.50
自引率
0.00%
发文量
436
期刊介绍: JMCR is an open access, peer-reviewed online journal that will consider any original case report that expands the field of general medical knowledge. Reports should show one of the following: 1. Unreported or unusual side effects or adverse interactions involving medications 2. Unexpected or unusual presentations of a disease 3. New associations or variations in disease processes 4. Presentations, diagnoses and/or management of new and emerging diseases 5. An unexpected association between diseases or symptoms 6. An unexpected event in the course of observing or treating a patient 7. Findings that shed new light on the possible pathogenesis of a disease or an adverse effect
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