Francisco González-Espinosa , Vincenzo Di Pilato , Luca Calabrese , Elisa Costa , Agustina Costa , Gabriel Gutkind , Daniela Cejas , Marcela Radice
{"title":"从阿根廷肠杆菌中回收的 blaNDM-5-harbouring 质粒的整体基因组描述。","authors":"Francisco González-Espinosa , Vincenzo Di Pilato , Luca Calabrese , Elisa Costa , Agustina Costa , Gabriel Gutkind , Daniela Cejas , Marcela Radice","doi":"10.1016/j.jgar.2024.10.258","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>To characterise four <em>bla</em><sub>NDM-5</sub>-harbouring plasmids recovered in <em>Enterobacterales</em> isolated in Argentina.</div></div><div><h3>Methods</h3><div>DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and visualised by Proksee and Clinker. <em>bla</em><sub>NDM-5</sub>-harbouring plasmids were compared with similar deposited plasmids using PLSDB.</div></div><div><h3>Results</h3><div>Two plasmids belonged to incompatibility group IncFII where <em>bla</em><sub>NDM-5</sub> was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥90% shared hashes (sh)), with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring <em>bla</em><sub>NDM-5</sub> in a novel variant of the genetic context. Both multi-replicon plasmids presented two ARIs, one containing <em>bla</em><sub>NDM-5</sub> in addition to another antimicrobial resistance marker (ARM), and the second ARI carrying <em>bla</em><sub>CTX−M-15</sub> and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (<em>n</em> = 5) displayed less than 60% shared hashes and showed the same Inc groups but lacked ARM.</div></div><div><h3>Conclusions</h3><div>This study broadens the limited understanding of <em>bla</em><sub>NDM-5</sub>-harbouring plasmids in Latin America. Furthermore, it represents the first description of <em>bla</em><sub>NDM-5</sub> in a novel variant of common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any ARM.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 224-226"},"PeriodicalIF":3.7000,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina.\",\"authors\":\"Francisco González-Espinosa , Vincenzo Di Pilato , Luca Calabrese , Elisa Costa , Agustina Costa , Gabriel Gutkind , Daniela Cejas , Marcela Radice\",\"doi\":\"10.1016/j.jgar.2024.10.258\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>To characterise four <em>bla</em><sub>NDM-5</sub>-harbouring plasmids recovered in <em>Enterobacterales</em> isolated in Argentina.</div></div><div><h3>Methods</h3><div>DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and visualised by Proksee and Clinker. <em>bla</em><sub>NDM-5</sub>-harbouring plasmids were compared with similar deposited plasmids using PLSDB.</div></div><div><h3>Results</h3><div>Two plasmids belonged to incompatibility group IncFII where <em>bla</em><sub>NDM-5</sub> was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥90% shared hashes (sh)), with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring <em>bla</em><sub>NDM-5</sub> in a novel variant of the genetic context. Both multi-replicon plasmids presented two ARIs, one containing <em>bla</em><sub>NDM-5</sub> in addition to another antimicrobial resistance marker (ARM), and the second ARI carrying <em>bla</em><sub>CTX−M-15</sub> and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (<em>n</em> = 5) displayed less than 60% shared hashes and showed the same Inc groups but lacked ARM.</div></div><div><h3>Conclusions</h3><div>This study broadens the limited understanding of <em>bla</em><sub>NDM-5</sub>-harbouring plasmids in Latin America. Furthermore, it represents the first description of <em>bla</em><sub>NDM-5</sub> in a novel variant of common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any ARM.</div></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"39 \",\"pages\":\"Pages 224-226\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-10-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524004363\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524004363","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina.
Objective
To characterise four blaNDM-5-harbouring plasmids recovered in Enterobacterales isolated in Argentina.
Methods
DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and visualised by Proksee and Clinker. blaNDM-5-harbouring plasmids were compared with similar deposited plasmids using PLSDB.
Results
Two plasmids belonged to incompatibility group IncFII where blaNDM-5 was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥90% shared hashes (sh)), with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring blaNDM-5 in a novel variant of the genetic context. Both multi-replicon plasmids presented two ARIs, one containing blaNDM-5 in addition to another antimicrobial resistance marker (ARM), and the second ARI carrying blaCTX−M-15 and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (n = 5) displayed less than 60% shared hashes and showed the same Inc groups but lacked ARM.
Conclusions
This study broadens the limited understanding of blaNDM-5-harbouring plasmids in Latin America. Furthermore, it represents the first description of blaNDM-5 in a novel variant of common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any ARM.
期刊介绍:
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.