从阿根廷肠杆菌中回收的 blaNDM-5-harbouring 质粒的整体基因组描述。

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
Francisco González-Espinosa , Vincenzo Di Pilato , Luca Calabrese , Elisa Costa , Agustina Costa , Gabriel Gutkind , Daniela Cejas , Marcela Radice
{"title":"从阿根廷肠杆菌中回收的 blaNDM-5-harbouring 质粒的整体基因组描述。","authors":"Francisco González-Espinosa ,&nbsp;Vincenzo Di Pilato ,&nbsp;Luca Calabrese ,&nbsp;Elisa Costa ,&nbsp;Agustina Costa ,&nbsp;Gabriel Gutkind ,&nbsp;Daniela Cejas ,&nbsp;Marcela Radice","doi":"10.1016/j.jgar.2024.10.258","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>To characterise four <em>bla</em><sub>NDM-5</sub>-harbouring plasmids recovered in <em>Enterobacterales</em> isolated in Argentina.</div></div><div><h3>Methods</h3><div>DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and visualised by Proksee and Clinker. <em>bla</em><sub>NDM-5</sub>-harbouring plasmids were compared with similar deposited plasmids using PLSDB.</div></div><div><h3>Results</h3><div>Two plasmids belonged to incompatibility group IncFII where <em>bla</em><sub>NDM-5</sub> was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥90% shared hashes (sh)), with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring <em>bla</em><sub>NDM-5</sub> in a novel variant of the genetic context. Both multi-replicon plasmids presented two ARIs, one containing <em>bla</em><sub>NDM-5</sub> in addition to another antimicrobial resistance marker (ARM), and the second ARI carrying <em>bla</em><sub>CTX−M-15</sub> and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (<em>n</em> = 5) displayed less than 60% shared hashes and showed the same Inc groups but lacked ARM.</div></div><div><h3>Conclusions</h3><div>This study broadens the limited understanding of <em>bla</em><sub>NDM-5</sub>-harbouring plasmids in Latin America. Furthermore, it represents the first description of <em>bla</em><sub>NDM-5</sub> in a novel variant of common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any ARM.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":"39 ","pages":"Pages 224-226"},"PeriodicalIF":3.7000,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina.\",\"authors\":\"Francisco González-Espinosa ,&nbsp;Vincenzo Di Pilato ,&nbsp;Luca Calabrese ,&nbsp;Elisa Costa ,&nbsp;Agustina Costa ,&nbsp;Gabriel Gutkind ,&nbsp;Daniela Cejas ,&nbsp;Marcela Radice\",\"doi\":\"10.1016/j.jgar.2024.10.258\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><div>To characterise four <em>bla</em><sub>NDM-5</sub>-harbouring plasmids recovered in <em>Enterobacterales</em> isolated in Argentina.</div></div><div><h3>Methods</h3><div>DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and visualised by Proksee and Clinker. <em>bla</em><sub>NDM-5</sub>-harbouring plasmids were compared with similar deposited plasmids using PLSDB.</div></div><div><h3>Results</h3><div>Two plasmids belonged to incompatibility group IncFII where <em>bla</em><sub>NDM-5</sub> was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥90% shared hashes (sh)), with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring <em>bla</em><sub>NDM-5</sub> in a novel variant of the genetic context. Both multi-replicon plasmids presented two ARIs, one containing <em>bla</em><sub>NDM-5</sub> in addition to another antimicrobial resistance marker (ARM), and the second ARI carrying <em>bla</em><sub>CTX−M-15</sub> and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (<em>n</em> = 5) displayed less than 60% shared hashes and showed the same Inc groups but lacked ARM.</div></div><div><h3>Conclusions</h3><div>This study broadens the limited understanding of <em>bla</em><sub>NDM-5</sub>-harbouring plasmids in Latin America. Furthermore, it represents the first description of <em>bla</em><sub>NDM-5</sub> in a novel variant of common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any ARM.</div></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":\"39 \",\"pages\":\"Pages 224-226\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-10-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524004363\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524004363","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

摘要

目的鉴定在阿根廷分离的肠杆菌中发现的四种与 blaNDM-5 相关的质粒:DNA由Illumina和牛津纳米孔技术测序,用Unicycler组装,用PlasmidFinder、MOB-Typer、IslandViewer4和Resfinder分析,用Proksee和Clinker可视化。结果:两个质粒属于不相容组IncFII,其中blaNDM-5位于先前描述的遗传背景中,浸没在抗菌药耐药性岛(ARI)中。本地 IncFII 质粒与 PLSDB 中的 4 个质粒具有高度相似性(共享哈希值(sh)≥ 90%)。另外两个本地质粒属于多复制子组 IncFIB-HI1B,以一种新的基因变异形式携带 blaNDM-5。这两个多复制子质粒都有两个ARI,其中一个含有blaNDM-5和其他ARM;第二个ARI含有blaCTX-M-15和一个1类整合子。存入 PLSDB 的质粒与本地多重复制质粒的相似度较低。最相似的质粒(n:5)的相似度低于 60%,显示出相同的 Inc 群,但缺乏 ARM:这项研究拓宽了人们对拉丁美洲 blaNDM-5 相关质粒的有限了解。此外,该研究首次描述了blaNDM-5在共同遗传平台中的新型变体及其在多重复制的IncFIB-IncHI1B质粒中的位置,而这些质粒以前未与任何抗菌药耐药性标记(ARM)相关联。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina.

Objective

To characterise four blaNDM-5-harbouring plasmids recovered in Enterobacterales isolated in Argentina.

Methods

DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and visualised by Proksee and Clinker. blaNDM-5-harbouring plasmids were compared with similar deposited plasmids using PLSDB.

Results

Two plasmids belonged to incompatibility group IncFII where blaNDM-5 was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥90% shared hashes (sh)), with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring blaNDM-5 in a novel variant of the genetic context. Both multi-replicon plasmids presented two ARIs, one containing blaNDM-5 in addition to another antimicrobial resistance marker (ARM), and the second ARI carrying blaCTX−M-15 and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (n = 5) displayed less than 60% shared hashes and showed the same Inc groups but lacked ARM.

Conclusions

This study broadens the limited understanding of blaNDM-5-harbouring plasmids in Latin America. Furthermore, it represents the first description of blaNDM-5 in a novel variant of common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any ARM.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信