González-Espinosa Francisco, Di Pilato Vincenzo, Calabrese Luca, Costa Elisa, Costa Agustina, Gutkind Gabriel, Cejas Daniela, Radice Marcela
{"title":"从阿根廷肠杆菌中回收的 blaNDM-5-harbouring 质粒的整体基因组描述。","authors":"González-Espinosa Francisco, Di Pilato Vincenzo, Calabrese Luca, Costa Elisa, Costa Agustina, Gutkind Gabriel, Cejas Daniela, Radice Marcela","doi":"10.1016/j.jgar.2024.10.258","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To characterise four bla<sub>NDM-5</sub>-harbouring plasmids recovered in Enterobacterales isolated in Argentina.</p><p><strong>Methods: </strong>DNA was sequenced by Illumina and Oxford Nanopore technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4 and Resfinder, and visualised by Proksee and Clinker. bla<sub>NDM-5</sub>-harbouring plasmids were compared with deposited similar ones using PLSDB.</p><p><strong>Results: </strong>two plasmids belonged to incompatibility group IncFII where bla<sub>NDM-5</sub> was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥ 90% shared hashes (sh)) with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring bla<sub>NDM-5</sub> in a novel variant of the genetic context. Both multi-replicon plasmids, presented two ARI, one containing bla<sub>NDM-5</sub> in addition to other ARM; and the second ARI carrying bla<sub>CTX-M-15</sub> and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (n:5) displayed less than 60% sh showing the same Inc groups but lacking ARM.</p><p><strong>Conclusion: </strong>This study broadens the limited understanding of bla<sub>NDM-5</sub>-harbouring plasmids in Latin America. Furthermore, it represents the first description of bla<sub>NDM-5</sub> in a novel variant of the common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any antimicrobial resistance marker (ARM).</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integral genomic description of bla<sub>NDM-5</sub>-harbouring plasmids recovered from Enterobacterales in Argentina.\",\"authors\":\"González-Espinosa Francisco, Di Pilato Vincenzo, Calabrese Luca, Costa Elisa, Costa Agustina, Gutkind Gabriel, Cejas Daniela, Radice Marcela\",\"doi\":\"10.1016/j.jgar.2024.10.258\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To characterise four bla<sub>NDM-5</sub>-harbouring plasmids recovered in Enterobacterales isolated in Argentina.</p><p><strong>Methods: </strong>DNA was sequenced by Illumina and Oxford Nanopore technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4 and Resfinder, and visualised by Proksee and Clinker. bla<sub>NDM-5</sub>-harbouring plasmids were compared with deposited similar ones using PLSDB.</p><p><strong>Results: </strong>two plasmids belonged to incompatibility group IncFII where bla<sub>NDM-5</sub> was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥ 90% shared hashes (sh)) with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring bla<sub>NDM-5</sub> in a novel variant of the genetic context. Both multi-replicon plasmids, presented two ARI, one containing bla<sub>NDM-5</sub> in addition to other ARM; and the second ARI carrying bla<sub>CTX-M-15</sub> and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (n:5) displayed less than 60% sh showing the same Inc groups but lacking ARM.</p><p><strong>Conclusion: </strong>This study broadens the limited understanding of bla<sub>NDM-5</sub>-harbouring plasmids in Latin America. Furthermore, it represents the first description of bla<sub>NDM-5</sub> in a novel variant of the common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any antimicrobial resistance marker (ARM).</p>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-10-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jgar.2024.10.258\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jgar.2024.10.258","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Integral genomic description of blaNDM-5-harbouring plasmids recovered from Enterobacterales in Argentina.
Objective: To characterise four blaNDM-5-harbouring plasmids recovered in Enterobacterales isolated in Argentina.
Methods: DNA was sequenced by Illumina and Oxford Nanopore technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4 and Resfinder, and visualised by Proksee and Clinker. blaNDM-5-harbouring plasmids were compared with deposited similar ones using PLSDB.
Results: two plasmids belonged to incompatibility group IncFII where blaNDM-5 was located in a previously described genetic context, immersed in an antimicrobial resistance island (ARI). Local IncFII plasmids displayed high similarity (≥ 90% shared hashes (sh)) with four deposited in PLSDB. The other two local plasmids belonged to the multi-replicon group IncFIB-HI1B, harbouring blaNDM-5 in a novel variant of the genetic context. Both multi-replicon plasmids, presented two ARI, one containing blaNDM-5 in addition to other ARM; and the second ARI carrying blaCTX-M-15 and a class 1 integron. Plasmids deposited in PLSDB showed low similarity to local multi-replicon plasmids. The most similar plasmids (n:5) displayed less than 60% sh showing the same Inc groups but lacking ARM.
Conclusion: This study broadens the limited understanding of blaNDM-5-harbouring plasmids in Latin America. Furthermore, it represents the first description of blaNDM-5 in a novel variant of the common genetic platform and its location in multi-replicon IncFIB-IncHI1B plasmids, which were not previously associated with any antimicrobial resistance marker (ARM).
期刊介绍：
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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