突触后BMP信号调节果蝇幼虫肌肉的肌核特性。

IF 7.4 1区 生物学 Q1 CELL BIOLOGY
Journal of Cell Biology Pub Date : 2025-01-06 Epub Date: 2024-10-30 DOI:10.1083/jcb.202404052
Victoria E von Saucken, Stefanie E Windner, Giovanna Armetta, Mary K Baylies
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引用次数: 0

摘要

哺乳动物的肌纤维中含有大量不同的(肌)核。在神经肌肉接头(NMJ)处,肌核有专门的定位和基因表达。然而,目前仍不清楚肌核是如何被招募的,以及是什么调控肌核在 NMJ 的输出。在这里,我们确定了位于果蝇幼虫 NMJ 附近的肌核的特殊属性。这些突触肌核的大小相对于其周围的细胞质域增大(大小缩放),DNA含量增加(倍性),转录因子pMad的水平增加,而pMad是BMP信号活动的读数。我们的遗传操作表明,局部 BMP 信号影响肌肉大小、核大小、倍性以及 NMJ 大小和功能。作为佐证,RNA 测序分析显示 pMad 可调控涉及肌肉生长、倍性(即 E2f1)和神经传递的基因。我们的数据表明,肌肉 BMP 信号指示突触肌核输出,从而积极塑造 NMJ 突触。这项研究加深了我们对肌核异质性如何支持局部信号需求以微调细胞功能和 NMJ 活动的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Postsynaptic BMP signaling regulates myonuclear properties in Drosophila larval muscles.

The syncytial mammalian muscle fiber contains a heterogeneous population of (myo)nuclei. At the neuromuscular junction (NMJ), myonuclei have specialized positioning and gene expression. However, it remains unclear how myonuclei are recruited and what regulates myonuclear output at the NMJ. Here, we identify specific properties of myonuclei located near the Drosophila larval NMJ. These synaptic myonuclei have increased size in relation to their surrounding cytoplasmic domain (size scaling), increased DNA content (ploidy), and increased levels of transcription factor pMad, a readout for BMP signaling activity. Our genetic manipulations show that local BMP signaling affects muscle size, nuclear size, ploidy, and NMJ size and function. In support, RNA sequencing analysis reveals that pMad regulates genes involved in muscle growth, ploidy (i.e., E2f1), and neurotransmission. Our data suggest that muscle BMP signaling instructs synaptic myonuclear output that positively shapes the NMJ synapse. This study deepens our understanding of how myonuclear heterogeneity supports local signaling demands to fine tune cellular function and NMJ activity.

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来源期刊
Journal of Cell Biology
Journal of Cell Biology 生物-细胞生物学
CiteScore
12.60
自引率
2.60%
发文量
213
审稿时长
1 months
期刊介绍: The Journal of Cell Biology (JCB) is a comprehensive journal dedicated to publishing original discoveries across all realms of cell biology. We invite papers presenting novel cellular or molecular advancements in various domains of basic cell biology, along with applied cell biology research in diverse systems such as immunology, neurobiology, metabolism, virology, developmental biology, and plant biology. We enthusiastically welcome submissions showcasing significant findings of interest to cell biologists, irrespective of the experimental approach.
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