María G Ramírez-Ledesma, Berenice Bermudes-Valencia, Rosa M Balderas-Parada, Susana G Salazar-Ramírez, Ruth Reyes-Cortés, Francisco J Magos-Vázquez, José J Torres-Hernández, Eva E Avila
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In seven vaginal swabs from the control group (formed by 19 donors without vaginal infection symptoms), we detected at least one pathogen per sample and observed ETs; thus, these donors were considered asymptomatic. The remaining 12 donors were confirmed to be healthy, as their exudates did not present any tested pathogens, sign of inflammation or ETs. 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引用次数: 0
摘要
阴道感染是一个公共卫生问题,由于其产生的炎症加剧,会导致严重的健康并发症。阴道炎症也可能发生在一些非感染过程中,如非感染性阴道炎和细胞溶解性阴道炎。免疫系统细胞通过各种机制对感染做出反应,如形成细胞外捕获物(ETs),这是一种与效应蛋白相关的 DNA 网络。许多病原体会在体外诱导 ETs 的形成,某些自然感染也会出现这种情况。最近的一份报告显示,人体阴道感染在体内会产生 ETs。因此,在本研究中,我们旨在从 40 名被诊断为感染性(即细菌性阴道炎、念珠菌病和滴虫病)和非感染性(即非感染性阴道炎和细胞溶解性阴道炎)阴道炎症的供体样本中鉴定 ETs。我们通过识别与 DNA 网络相关的 LL-37 肽来观察 ET。在对照组(由 19 名无阴道感染症状的捐献者组成)的 7 份阴道拭子中,我们在每个样本中至少检测到一种病原体,并观察到了 ET;因此,这些捐献者被视为无症状。其余 12 名捐献者被证实是健康的,因为他们的渗出物中没有任何经检测的病原体、炎症迹象或 ET。阴道炎症过程中的 ET 会加重炎症,但也可能有助于控制感染。
Extracellular Traps in Patients Diagnosed With Bacterial Vaginosis, Trichomoniasis, Candidiasis, Noninfectious Vaginitis and Cytolytic Vaginosis.
Vaginal infections are a public health problem associated with serious health complications due to the exacerbated inflammation they generate. Vaginal inflammation may also occur in some noninfectious processes, such as noninfectious vaginitis and cytolytic vaginosis. Immune system cells respond to infections through various mechanisms, such as the formation of extracellular traps (ETs), which are DNA networks associated with effector proteins. Many pathogens induce ETs formation in vitro, as occurs in some natural infections. A recent report indicates that human vaginal infections in vivo generate ETs. Therefore, in this study, we aimed to identify ETs in samples from 40 donors who were diagnosed with infectious (i.e., bacterial vaginosis, candidiasis and trichomoniasis) and noninfectious (i.e., noninfectious vaginitis and cytolytic vaginosis) vaginal inflammation. We were able to observe ETs by identifying the LL-37 peptide, which is associated with DNA networks. In seven vaginal swabs from the control group (formed by 19 donors without vaginal infection symptoms), we detected at least one pathogen per sample and observed ETs; thus, these donors were considered asymptomatic. The remaining 12 donors were confirmed to be healthy, as their exudates did not present any tested pathogens, sign of inflammation or ETs. ETs in vaginal inflammatory processes can worsen inflammation but may also help control infection.
期刊介绍:
International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.