地克珠利纳米乳剂在控制肉鸡天牛艾美耳病方面的有效性和安全性。

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Azza A El-Sawah, Shawky M Aboelhadid, El-Shymaa N El-Nahass, Hassan E Helal
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引用次数: 0

摘要

背景:纳米技术具有减少药物用量、提高药效的潜力;因此,本研究旨在合成地克珠利纳米乳剂,并评估其抗肉鸡实验性球虫病的性能:方法:配制了地克珠利纳米乳剂(DZN),并用 zeta seizer 和 zeta 电位对其进行了表征。对配制好的 DZN 进行了体内试验,以评估其对感染了天牛埃默氏菌的雏鸡的效果。DZN 和 DZ 分别用于治疗和预防两种方案。210 只一天龄的肉鸡被平均分成 6 组。对照组为阴性未感染未处理组和阳性感染未处理组(G1 和 G2)。治疗组(G3 和 G4)在出现球虫病临床症状后用 DZ 和 DZN 治疗,持续 5 天。预防组(G5 和 G6)在感染前 3 天接受 DZ 和 DZN 治疗,感染后继续治疗 5 天。商业来源的 DZ 剂量为 10 mg/mL,制备的 DZN 剂量为 2.5 mg/mL。所有组(阴性对照组除外)口服感染后,随访球虫病的临床症状、死亡率、卵囊数、性能、血液学和生化指标以及组织病理学病变:治疗组显示,两个治疗组(DZ 和 DZN)的结果相似,包括体重增加良好、病变盲肠评分低、每日卵囊脱落数和总脱落数低以及死亡率低。在生化指标方面,所有指标在感染期间都受到影响,但在感染后第 12 天后又得到恢复。然而,在预防组中,临床症状轻微,血象和生化指标几乎与未感染的阴性对照组相同:结论:标准剂量 DZ 的四分之一的 DZN 与 10 毫克/毫升的 DZ 产生了相同的效果。此外,DZN 不会损害地克珠利对雏鸡的典型安全性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Efficacy and safety of diclazuril nanoemulsion in control of Eimeria tenella in broilers.

Background: Nanotechnology has the potential to reduce drug dosage while increasing efficacy; thus, the current work intends to synthesize diclazuril nanoemulsion and assess its performance against experimental coccidiosis in broilers.

Methods: Diclazuril nanoemulsion (DZN) was formulated and characterized by zeta seizer and zeta potential. The formulated DZN was evaluated in vivo against Eimeria tenella infected chicks. DZN and DZ were used in 2 programs; therapeutic and prophylactic. A total of 210 one-day-old broiler chicks were distributed equally into six groups. The controls were negative uninfected untreated and positive infected untreated (G1 & G2). Therapeutic groups (G3 & G4) treated by DZ and DZN after appearance of the clinical signs of coccidiosis and continued for 5 days. Prophylaxis groups (G5 & G6) received DZ and DZN at 3 days before challenge and continued for 5 days after infection. The treatments dosages were 10 mg/mL for DZ of commercial origin and 2.5 mg/mL for the prepared DZN. All groups (except negative control) orally infected then followed up for clinical signs of coccidiosis, mortality rate, oocysts count, performance, hematological and biochemical parameters in addition to histopathological lesions.

Results: The therapeutic groups showed that both treated groups (DZ and DZN) revealed similar results including good body weight gain, a low lesion caecal score, a low daily and total oocyst shedding count, and a low mortality rate. Regarding the biochemical parameters, all parameters were affected during infection then restored after the 12th day post infection. However, in the prophylactic groups, showed mild clinical signs and the blood pictures and biochemical parameters were nearly like the control negative without infection.

Conclusion: DZN at a quarter dose of standard DZ produced the same outcomes as DZ at 10 mg/mL. Furthermore, DZN does not impair the typical safety of diclazuril in treated chicks.

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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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