假单胞菌产生的各种代谢物对扫帚菜具有除草活性

IF 6.1 1区 生物学 Q1 MICROBIOLOGY
Tristan Lurthy , Florence Gerin, Marjolaine Rey , Pierre-Edouard Mercier , Gilles Comte , Florence Wisniewski-Dyé , Claire Prigent-Combaret
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引用次数: 0

摘要

假单胞菌以其促进植物生长的特性和对微生物病原体的生物防治能力而闻名。最近,假单胞菌保护农作物免受寄生植物侵害的潜力引起了人们的关注。本研究调查了不同假单胞菌株抑制扫帚霉生长和保护寄主植物免受杂草侵扰的潜力。研究人员利用果糖、丙酮酸盐、富马酸盐和苹果酸盐等不同碳源培养了四株假单胞菌,其中两株为荧光假单胞菌 JV391D17 和 JV391D10,一株为绿假单胞菌 JV395B,还有一株为奥加假单胞菌 F113,以提高假单胞菌菌株产生的潜在裸冠菊生长抑制(OGI)特化代谢物的多样性。为了鉴定特定的 OGI 代谢物,采用了全局和目标代谢组学方法。碳源和假单胞菌遗传多样性都对 OGI 代谢物的产生有显著影响。P. chlororaphis JV395B 和 P. ogarae F113 产生了独特的 OGI 代谢物,它们分别属于不同的化学家族,如羟基吩嗪类化合物和氯代葡萄糖苷醇化合物。此外,代谢组分析还发现了一种未注释的潜在 OGI 离子 M375T65。所有假单胞菌菌株都能产生这种离子,但在 JV395B 中发现这种离子过度积累,这可能是其 OGI 活性更强的原因。然后,进行了温室实验,以评估所选菌株的生物防治功效:实验结果表明,这些菌株,尤其是 JV395B,在减少油菜籽中的扫帚霉侵染方面具有功效。这些研究结果表明,某些假单胞菌菌株通过产生代谢物,可以提供一种针对寄生植物的可持续生物防治策略。这种生物防治活性可以通过碳添加剂等环境因素进行优化。最终,这种方法有望替代化学除草剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pseudomonas produce various metabolites displaying herbicide activity against broomrape
Pseudomonads are well-known for their plant growth-promoting properties and biocontrol capabilities against microbial pathogens. Recently, their potential to protect crops from parasitic plants has garnered attention. This study investigates the potential of different Pseudomonas strains to inhibit broomrape growth and to protect host plants against weed infestation. Four Pseudomonas strains, two P. fluorescens JV391D17 and JV391D10, one P. chlororaphis JV395B and one P. ogarae F113 were cultivated using various carbon sources, including fructose, pyruvate, fumarate, and malate, to enhance the diversity of potential Orobanche growth inhibition (OGI)-specialized metabolites produced by Pseudomonas strains. Both global and targeted metabolomic approaches were utilized to identify specific OGI metabolites. Both carbon sources and Pseudomonas genetic diversity significantly influenced the production of OGI metabolites. P. chlororaphis JV395B and P. ogarae F113 produced unique OGI metabolites belonging to different chemical families, such as hydroxyphenazines and phloroglucinol compounds, respectively. Additionally, metabolomic analyses identified an unannotated potential OGI ion, M375T65. This ion was produced by all Pseudomonas strains but was found to be over-accumulated in JV395B, which likely explains its superior OGI activity. Then, greenhouse experiments were performed to evaluate the biocontrol efficacy of selected strains: they showed the efficacy of these strains, particularly JV395B, in reducing broomrape infestation in rapeseed. These findings suggest that certain Pseudomonas strains, through their metabolite production, can offer a sustainable biocontrol strategy against parasitic plants. This biocontrol activity can be optimized by environmental factors, such as carbon amendments. Ultimately, this approach presents a promising alternative to chemical herbicides.
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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