{"title":"微型双波长 β 校正分光光度计探针,通过形成氰基二硫腙加合物灵敏检测水中的氰化物","authors":"E.A. Bahaidarah , H. Alwael , M.M. Dabi , T.N. Abduljabbar , F.M. Alshareef , K.A. Alzahrani , N.A. Asiri , N.O. Maslamani , S.O. Bahaffi , M.S. El-Shahawi","doi":"10.1016/j.saa.2024.125314","DOIUrl":null,"url":null,"abstract":"<div><div>Cyanide toxicity in water significantly threatens public health and the environment. To address this, a miniaturized simple, low-cost, selective and sensitive direct dual wave β-correction spectrophotometric probe has been established for cyanide detection in water. The dual-wavelength β-correction spectrophotometry enhances the selectivity and sensitivity of the probe in the presence of interfering species. The assay relies a highly selective nucleophilic addition of cyanide ions to dithizone (H<sub>2</sub>Dz) as chromogenic reagent in aqueous media of pH 6.8–7.2, forming a red-colored cyano H<sub>2</sub>Dz adduct. The electronic spectrum of the formed adduct displays a sharp absorption peak at λ<sub>max</sub> = 480 nm, enabling precise colorimetric detection. The molar absorptivity and Sandell’s sensitivity index for the cyano H<sub>2</sub>Dz adduct with and without <em>β</em>-correction spectrophotometry were 5.62 × 10<sup>3</sup>, and 1.79 × 10<sup>3</sup> L mol<sup>−</sup><sup>1</sup> cm<sup>−</sup><sup>1</sup>, and 0.002 and 0.0033 µg cm<sup>−</sup><sup>2</sup>, respectively. Beer’s law and Ringbom’s plots are valid in the range 0.01–5.0 and 0.06–2.0 µg mL<sup>−</sup><sup>1</sup> CN<sup>–</sup> concentration, respectively. The limits of detection (LOD) and quantification (LOQ) improved from 1.03 × 10<sup>−1</sup> and 3.13 × 10<sup>−1</sup> μg/mL using ordinary spectrophotometry to 2.8 × 10<sup>−2</sup> and 8.7 × 10<sup>−2</sup> μg mL<sup>−1</sup> employing β-correction spectrophotometry. The probe offers rapid response, good anti-interference ability, reproducibility, and cost-effectiveness. The probe was successfully applied for detection of trace levels of cyanide ions in water with good repeatability. It has been also validated in water samples with good recoveries (99.2 ± 5.02 %). The experimental <em>Student t</em> test (<em>t<sub>exp</sub></em> = 1.2–1.5) was lower than the critical (<em>t<sub>exp</sub> =</em> 2.78) at 95 % probability <em>(</em>n = 5). The stoichiometry and mechanism of formation of the cyano adduct were assigned and addressed.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Miniaturized dual-wavelength β-correction spectrophotometric probe for sensitive detection of cyanide in water via formation of cyano dithizone adduct\",\"authors\":\"E.A. Bahaidarah , H. Alwael , M.M. Dabi , T.N. Abduljabbar , F.M. Alshareef , K.A. Alzahrani , N.A. Asiri , N.O. Maslamani , S.O. Bahaffi , M.S. El-Shahawi\",\"doi\":\"10.1016/j.saa.2024.125314\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Cyanide toxicity in water significantly threatens public health and the environment. To address this, a miniaturized simple, low-cost, selective and sensitive direct dual wave β-correction spectrophotometric probe has been established for cyanide detection in water. The dual-wavelength β-correction spectrophotometry enhances the selectivity and sensitivity of the probe in the presence of interfering species. The assay relies a highly selective nucleophilic addition of cyanide ions to dithizone (H<sub>2</sub>Dz) as chromogenic reagent in aqueous media of pH 6.8–7.2, forming a red-colored cyano H<sub>2</sub>Dz adduct. The electronic spectrum of the formed adduct displays a sharp absorption peak at λ<sub>max</sub> = 480 nm, enabling precise colorimetric detection. The molar absorptivity and Sandell’s sensitivity index for the cyano H<sub>2</sub>Dz adduct with and without <em>β</em>-correction spectrophotometry were 5.62 × 10<sup>3</sup>, and 1.79 × 10<sup>3</sup> L mol<sup>−</sup><sup>1</sup> cm<sup>−</sup><sup>1</sup>, and 0.002 and 0.0033 µg cm<sup>−</sup><sup>2</sup>, respectively. Beer’s law and Ringbom’s plots are valid in the range 0.01–5.0 and 0.06–2.0 µg mL<sup>−</sup><sup>1</sup> CN<sup>–</sup> concentration, respectively. The limits of detection (LOD) and quantification (LOQ) improved from 1.03 × 10<sup>−1</sup> and 3.13 × 10<sup>−1</sup> μg/mL using ordinary spectrophotometry to 2.8 × 10<sup>−2</sup> and 8.7 × 10<sup>−2</sup> μg mL<sup>−1</sup> employing β-correction spectrophotometry. The probe offers rapid response, good anti-interference ability, reproducibility, and cost-effectiveness. The probe was successfully applied for detection of trace levels of cyanide ions in water with good repeatability. It has been also validated in water samples with good recoveries (99.2 ± 5.02 %). The experimental <em>Student t</em> test (<em>t<sub>exp</sub></em> = 1.2–1.5) was lower than the critical (<em>t<sub>exp</sub> =</em> 2.78) at 95 % probability <em>(</em>n = 5). The stoichiometry and mechanism of formation of the cyano adduct were assigned and addressed.</div></div>\",\"PeriodicalId\":433,\"journal\":{\"name\":\"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-10-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S138614252401480X\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"SPECTROSCOPY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S138614252401480X","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}
Miniaturized dual-wavelength β-correction spectrophotometric probe for sensitive detection of cyanide in water via formation of cyano dithizone adduct
Cyanide toxicity in water significantly threatens public health and the environment. To address this, a miniaturized simple, low-cost, selective and sensitive direct dual wave β-correction spectrophotometric probe has been established for cyanide detection in water. The dual-wavelength β-correction spectrophotometry enhances the selectivity and sensitivity of the probe in the presence of interfering species. The assay relies a highly selective nucleophilic addition of cyanide ions to dithizone (H2Dz) as chromogenic reagent in aqueous media of pH 6.8–7.2, forming a red-colored cyano H2Dz adduct. The electronic spectrum of the formed adduct displays a sharp absorption peak at λmax = 480 nm, enabling precise colorimetric detection. The molar absorptivity and Sandell’s sensitivity index for the cyano H2Dz adduct with and without β-correction spectrophotometry were 5.62 × 103, and 1.79 × 103 L mol−1 cm−1, and 0.002 and 0.0033 µg cm−2, respectively. Beer’s law and Ringbom’s plots are valid in the range 0.01–5.0 and 0.06–2.0 µg mL−1 CN– concentration, respectively. The limits of detection (LOD) and quantification (LOQ) improved from 1.03 × 10−1 and 3.13 × 10−1 μg/mL using ordinary spectrophotometry to 2.8 × 10−2 and 8.7 × 10−2 μg mL−1 employing β-correction spectrophotometry. The probe offers rapid response, good anti-interference ability, reproducibility, and cost-effectiveness. The probe was successfully applied for detection of trace levels of cyanide ions in water with good repeatability. It has been also validated in water samples with good recoveries (99.2 ± 5.02 %). The experimental Student t test (texp = 1.2–1.5) was lower than the critical (texp = 2.78) at 95 % probability (n = 5). The stoichiometry and mechanism of formation of the cyano adduct were assigned and addressed.
期刊介绍:
Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science.
The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments.
Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate.
Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to:
Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences,
Novel experimental techniques or instrumentation for molecular spectroscopy,
Novel theoretical and computational methods,
Novel applications in photochemistry and photobiology,
Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.