社区和医院获得性尿路感染中的大肠埃希菌、肺炎克雷伯菌和奇异变形杆菌的广谱β-内酰胺酶变体

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-10-16 DOI:10.1016/j.genrep.2024.102065

Lubna Razaq , Fakhur Uddin , Sanum Ali , Shahzad Ali , Rizwana Kausar , Muhammad Sohail

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引用次数: 0

摘要

产广谱-β-内酰胺酶（ESBL）的肠杆菌，尤其是大肠埃希菌、肺炎克雷伯菌和奇异变形杆菌，在世界各地非常普遍。然而，关于医院获得性（HA）和社区获得性（CA）尿路感染（UTI）中肠杆菌ESBL变异株的流行情况，在发展中国家却鲜有报道。本研究分析了产ESBL肠杆菌的存在、其耐药性模式和ESBL变异体。在 279 份尿液样本中，144 份（51.6%）来自 CA-UTI 患者，135 份（48.4%）来自 HA-UTI 患者。从这些样本中，CA 组和 HA-UTI 组分别分离出 145 和 135 株肠杆菌。其中以大肠杆菌为主（202/280；72.2%），其次是肺炎双球菌（53/280；18.9%）和奇异变形杆菌（25/280；8.9%）。在 HA-UTI 分离物中，ESBL 生产者较多（131/218；60.09%）。总的来说，blaSHV（48.2%）是最主要的基因，其次是 blaTEM（44%），而在 CA 和 HA-UTI 分离物中很少检测到 blaCTX-M（7.79%）。还观察到 blaTEM 和 blaSHV 共存的现象。这项研究强调了尿路病原体中不同的 ESBL 模式，有助于更新传染病控制和抗菌药物管理指南。

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Extended-spectrum β-lactamase variants in Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis from community- and hospital-acquired urinary tract infections

Extended-spectrum-β-lactamase (ESBL)-producing Enterobacterales, especially Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis, are highly prevalent in various parts of the world. However, the prevalence of ESBL variants in Enterobacterales from hospital-acquired (HA) and community-acquired (CA) urinary tract infections (UTI) had been infrequently reported in developing countries. This study analyzed the presence of ESBL-producing Enterobacterales, their resistance patterns, and ESBL variants. Of the 279 urine samples, 144 (51.6 %) were obtained from patients with CA-UTI and 135 (48.4 %) were obtained from patients with HA-UTI. From these samples, 145 and 135 Enterobacterales strains were isolated from the CA and HA-UTI groups, respectively. E. coli was the predominant (202/280; 72.2 %), followed by K. pneumoniae (53/280; 18.9 %) and P. mirabilis (25/280; 8.9 %). ESBL producers were higher (131/218; 60.09 %) in the HA-UTI isolates. Overall, bla_SHV (48.2 %) was the predominant gene, followed by bla_TEM (44 %), whereas bla_CTX-M was rarely detected (7.79 %) in the CA and HA-UTI isolates. bla_SHV-212, bla_SHV-229, bla_TEM-₁₀₃, and bla_TEM-₁₀₄ were common among the isolates. The coexistence of bla_TEM and bla_SHV was also observed. This study highlights a different ESBL pattern among uropathogens that can aid in updating infectious disease control and antimicrobial stewardship guidelines.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.