从儿科个体的淋巴母细胞中生成对照诱导多能干细胞系 (CBRCULi014-A)

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2024-10-16 DOI:10.1016/j.scr.2024.103587

Thiéry De Serres-Bérard , Valérie Pouliot , Jack Puymirat , Mohamed Chahine

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引用次数: 0

摘要

淋巴母细胞系是生成诱导多能干细胞（iPSCs）的一种现成且持续的资源，可对各种遗传疾病进行体外建模。在研究先天性疾病和婴幼儿疾病时，通常需要来自儿科个体的年龄匹配对照，但这些对照可能很少或难以获得。在这里，研究人员利用仙台病毒系统将重编程因子导入来自一名表面上健康的 4 岁女性的淋巴母细胞。生成的 iPSCs 强烈表达多能性细胞标记，并显示出强大的三系分化能力。因此，CBRCULi014-A 是用于儿科疾病研究的可靠对照 iPSC 株系。

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Generation of a control induced pluripotent stem cell line (CBRCULi014-A) derived from the lymphoblastoid cells of a pediatric individual

Lymphoblastoid cell lines serve as a readily and continuous resource for generating induced pluripotent stem cells (iPSCs), enabling the modeling of various genetic disorders in vitro. When investigating congenital and infantile diseases, age-matched controls derived from pediatric individuals are typically necessary, yet they may be scarce or difficult to obtain. Here, the Sendai virus system was employed to introduce reprogramming factors into lymphoblastoid cells derived from an apparently healthy 4-year-old female. The generated iPSCs strongly expressed pluripotency cell markers and displayed robust trilineage differentiation. CBRCULi014-A is therefore a reliable control iPSC line for pediatric disease investigation.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.