捕食性原生生物通过在植物生长早期丰富植物有益微生物来抑制细菌性枯萎病的发生

IF 3.9 2区 农林科学 Q1 AGRONOMY
Yuqi Song, Chen Liu, Keming Yang, Shiqi Sun, Lin Wang, Cansheng Yuan, Yangchun Xu, Wu Xiong, Qirong Shen, Zhong Wei
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引用次数: 0

摘要

目的植物健康与根圈微生物群落密切相关。捕食性原生动物可以调节根圈微生物,从而影响植物健康。然而,关于外源添加捕食性原生动物如何在植物生长过程中影响植物根瘤微生物群落的研究还很有限。QL92,它能有效抑制细菌枯萎病。结果我们发现,在番茄的幼苗期、开花期和结果期,Naegleria 的捕食性原生生物显著改变了根瘤菌群的群落结构和组成。此外,加入 Naegleria 后,番茄幼苗期蛋白菌门、革马拉菌门、硝化菌门以及大多数细菌属(尤其是假单胞菌属)的相对丰度都有所增加。接种 Naegleria 降低了茄形菌(Ralstonia solanacearum)病原体的密度,这与假单胞菌的相对数量呈负相关。总之,我们的研究强调了捕食性原生动物作为根圈细菌群落傀儡主人的应用,其丰富的植物有益微生物为管理根圈微生物群落提供了新的途径,以支持可持续农业中植物的健康生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Predatory protist promotes disease suppression against bacterial wilt through enriching plant beneficial microbes at the early stage of plant growth

Predatory protist promotes disease suppression against bacterial wilt through enriching plant beneficial microbes at the early stage of plant growth

Aims

Plant health is closely associated with the rhizosphere microbial community. Predatory protists can regulate the rhizosphere microbes and thereby affect plant health. However, there is limited research on how the exogenous addition of predatory protists influences plant rhizosphere microbiome across plant growth.

Methods

Here, we isolated a predatory protist species, named Naegleria sp. QL92, from healthy tomato rhizosphere soil, which can effectively suppress bacterial wilt. We investigated the impact of predatory protist addition on the rhizosphere bacterial community across tomato growth stages in pots.

Results

We found that the predatory protist of Naegleria significantly altered the community structure and composition of the rhizosphere bacteria during the seedling, flowering and fruiting stages of tomato growth. Moreover, the relative abundances of bacterial phylum of Proteobacteria, Gemmatimonadetes, and Nitrospirae as well as majority bacterial genera, especially Pseudomonas were increased during the seedling stage after Naegleria addition. Naegleria inoculation reduced the density of the Ralstonia solanacearum pathogen, which was negatively correlated with Pseudomonas relative abundance. The addition of Naegleria also increased the connections within rhizosphere bacterial communities, resulting in a complex microbial network.

Conclusions

Overall, our study highlighted the application of predatory protists as puppet masters of rhizosphere bacterial communities with enriching plant beneficial microbes, which offer new venues to manage rhizosphere microbial communities to support healthy plant growth in sustainable agriculture.

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来源期刊
Plant and Soil
Plant and Soil 农林科学-农艺学
CiteScore
8.20
自引率
8.20%
发文量
543
审稿时长
2.5 months
期刊介绍: Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.
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