{"title":"捕食性原生生物通过在植物生长早期丰富植物有益微生物来抑制细菌性枯萎病的发生","authors":"Yuqi Song, Chen Liu, Keming Yang, Shiqi Sun, Lin Wang, Cansheng Yuan, Yangchun Xu, Wu Xiong, Qirong Shen, Zhong Wei","doi":"10.1007/s11104-024-07052-4","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Aims</h3><p>Plant health is closely associated with the rhizosphere microbial community. Predatory protists can regulate the rhizosphere microbes and thereby affect plant health. However, there is limited research on how the exogenous addition of predatory protists influences plant rhizosphere microbiome across plant growth.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>Here, we isolated a predatory protist species, named <i>Naegleria</i> sp. QL92, from healthy tomato rhizosphere soil, which can effectively suppress bacterial wilt. We investigated the impact of predatory protist addition on the rhizosphere bacterial community across tomato growth stages in pots.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>We found that the predatory protist of <i>Naegleria</i> significantly altered the community structure and composition of the rhizosphere bacteria during the seedling, flowering and fruiting stages of tomato growth. Moreover, the relative abundances of bacterial phylum of Proteobacteria, Gemmatimonadetes, and Nitrospirae as well as majority bacterial genera, especially <i>Pseudomonas</i> were increased during the seedling stage after <i>Naegleria</i> addition. <i>Naegleria</i> inoculation reduced the density of the <i>Ralstonia solanacearum</i> pathogen, which was negatively correlated with <i>Pseudomonas</i> relative abundance. The addition of <i>Naegleria</i> also increased the connections within rhizosphere bacterial communities, resulting in a complex microbial network.</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p>Overall, our study highlighted the application of predatory protists as puppet masters of rhizosphere bacterial communities with enriching plant beneficial microbes, which offer new venues to manage rhizosphere microbial communities to support healthy plant growth in sustainable agriculture.</p>","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Predatory protist promotes disease suppression against bacterial wilt through enriching plant beneficial microbes at the early stage of plant growth\",\"authors\":\"Yuqi Song, Chen Liu, Keming Yang, Shiqi Sun, Lin Wang, Cansheng Yuan, Yangchun Xu, Wu Xiong, Qirong Shen, Zhong Wei\",\"doi\":\"10.1007/s11104-024-07052-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Aims</h3><p>Plant health is closely associated with the rhizosphere microbial community. Predatory protists can regulate the rhizosphere microbes and thereby affect plant health. However, there is limited research on how the exogenous addition of predatory protists influences plant rhizosphere microbiome across plant growth.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>Here, we isolated a predatory protist species, named <i>Naegleria</i> sp. QL92, from healthy tomato rhizosphere soil, which can effectively suppress bacterial wilt. We investigated the impact of predatory protist addition on the rhizosphere bacterial community across tomato growth stages in pots.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>We found that the predatory protist of <i>Naegleria</i> significantly altered the community structure and composition of the rhizosphere bacteria during the seedling, flowering and fruiting stages of tomato growth. Moreover, the relative abundances of bacterial phylum of Proteobacteria, Gemmatimonadetes, and Nitrospirae as well as majority bacterial genera, especially <i>Pseudomonas</i> were increased during the seedling stage after <i>Naegleria</i> addition. <i>Naegleria</i> inoculation reduced the density of the <i>Ralstonia solanacearum</i> pathogen, which was negatively correlated with <i>Pseudomonas</i> relative abundance. The addition of <i>Naegleria</i> also increased the connections within rhizosphere bacterial communities, resulting in a complex microbial network.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusions</h3><p>Overall, our study highlighted the application of predatory protists as puppet masters of rhizosphere bacterial communities with enriching plant beneficial microbes, which offer new venues to manage rhizosphere microbial communities to support healthy plant growth in sustainable agriculture.</p>\",\"PeriodicalId\":20223,\"journal\":{\"name\":\"Plant and Soil\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2024-10-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant and Soil\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s11104-024-07052-4\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-024-07052-4","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Predatory protist promotes disease suppression against bacterial wilt through enriching plant beneficial microbes at the early stage of plant growth
Aims
Plant health is closely associated with the rhizosphere microbial community. Predatory protists can regulate the rhizosphere microbes and thereby affect plant health. However, there is limited research on how the exogenous addition of predatory protists influences plant rhizosphere microbiome across plant growth.
Methods
Here, we isolated a predatory protist species, named Naegleria sp. QL92, from healthy tomato rhizosphere soil, which can effectively suppress bacterial wilt. We investigated the impact of predatory protist addition on the rhizosphere bacterial community across tomato growth stages in pots.
Results
We found that the predatory protist of Naegleria significantly altered the community structure and composition of the rhizosphere bacteria during the seedling, flowering and fruiting stages of tomato growth. Moreover, the relative abundances of bacterial phylum of Proteobacteria, Gemmatimonadetes, and Nitrospirae as well as majority bacterial genera, especially Pseudomonas were increased during the seedling stage after Naegleria addition. Naegleria inoculation reduced the density of the Ralstonia solanacearum pathogen, which was negatively correlated with Pseudomonas relative abundance. The addition of Naegleria also increased the connections within rhizosphere bacterial communities, resulting in a complex microbial network.
Conclusions
Overall, our study highlighted the application of predatory protists as puppet masters of rhizosphere bacterial communities with enriching plant beneficial microbes, which offer new venues to manage rhizosphere microbial communities to support healthy plant growth in sustainable agriculture.
期刊介绍:
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.