艾蒿中的β-石竹烯合成酶在酿酒酵母中高效生物合成β-石竹烯

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Synthetic and Systems Biotechnology Pub Date : 2024-09-22 DOI:10.1016/j.synbio.2024.09.005

Zhengping Li , Yuhong Gan , Changyu Gou , Qiongyu Ye , Yang Wu , Yuhong Wu , Tingxing Yang , Baolian Fan , Aijia Ji , Qi Shen , Lixin Duan

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引用次数: 0

摘要

阿尔基蒿 H. Lév.青蒿是一种重要的传统中药植物，以其挥发油而闻名，挥发油是青蒿的主要活性成分，包括单萜、倍半萜及其衍生物。尽管阿胶具有重要的药用价值，但其倍半萜类化合物的生物合成仍未得到充分探索。在这项研究中，我们从 A. argyi 中发现了四种 β-加里叶烯合成酶。本研究建立了一个高产的β-石竹烯工程酿酒酵母细胞工厂。通过将 ERG20 和 AarTPS88 与柔性连接子（GGGS）2 融合，并增强 MVA 通路（HIF-1、tHMGR 和 UPC2-1）的代谢通量，我们在 5 L 生物反应器中通过喂料批量发酵使 β-石竹烯的滴度达到 15.6 g/L。据我们所知，这是迄今为止报道的酵母中β-石竹烯的最高滴度。这项研究为β-石竹烯的工业化生产提供了宝贵的工具。

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Efficient biosynthesis of β-caryophyllene in Saccharomyces cerevisiae by β-caryophyllene synthase from Artemisia argyi

Artemisia argyi H. Lév. & Vaniot is an important traditional Chinese medicinal plant known for its volatile oils, which are the main active components of A. argyi, including monoterpenes, sesquiterpenes and their derivatives. Despite its medicinal significance, the biosynthesis of sesquiterpenoids in A. argyi remains underexplored. In this study, we identified four β-caryophyllene synthases from A. argyi. A high-yield β-caryophyllene engineered Saccharomyces cerevisiae cell factory has been built in this study. By fusing ERG20 and AarTPS88 with a flexible linker (GGGS)₂ and enhancing metabolic flux in the MVA pathway (HIF-1, tHMGR, and UPC2-1), we achieved a titer of β-caryophyllene reached 15.6 g/L by fed-batch fermentation in a 5 L bioreactor. To our knowledge, this represents the highest reported titer of β-caryophyllene in yeast to date. This study provides a valuable tool for the industrial-scale production of β-caryophyllene.

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来源期刊

Synthetic and Systems Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

6.90

自引率

12.50%

发文量

审稿时长

67 days

期刊介绍： Synthetic and Systems Biotechnology aims to promote the communication of original research in synthetic and systems biology, with strong emphasis on applications towards biotechnology. This journal is a quarterly peer-reviewed journal led by Editor-in-Chief Lixin Zhang. The journal publishes high-quality research; focusing on integrative approaches to enable the understanding and design of biological systems, and research to develop the application of systems and synthetic biology to natural systems. This journal will publish Articles, Short notes, Methods, Mini Reviews, Commentary and Conference reviews.