杂合子 KCNA2 基因敲除诱导多能干细胞系的产生

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2024-10-21 DOI:10.1016/j.scr.2024.103596

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引用次数: 0

摘要

从功能和结构角度看，KCNA2 基因是电压门控离子通道。KCNA2 参与多种功能，包括调节神经递质释放、心率、胰岛素分泌、神经元兴奋性、上皮电解质转运、平滑肌收缩和细胞体积。为了研究相关的病理生理机制，我们通过CRISPR/Cas9基因编辑技术产生了杂合子KCNA2基因敲除的人类诱导多能干细胞（iPSC）系。生成的 iPSCs 核型正常，不含基因整合的外显质粒，表达多能性标记，并保持三系分化潜能。

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Generation of heterozygous KCNA2 knockout induced pluripotent stem cell line

The KCNA2 gene is the voltage-gated ion channel from both functional and structural perspectives. KCNA2 is involved in diverse functions including the regulation of neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. To investigate the relevant pathophysiological mechanisms, we generated heterozygous KCNA2 knockout human induced pluripotent stem cell (iPSC) line via CRISPR/Cas9 gene editing. The generated iPSCs had a normal karyotype, were free of genetically integrated epitaxial plasmids, expressed pluripotency markers, and maintained trilineage differentiation potential.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.