Gilberto S. Coelho Junior , Vinicius P. Souza , Jan Kratzer , Jiří Dědina , Erico M.M. Flores
{"title":"微波诱导鱼组织燃烧后等离子体介导的汞蒸气生成及原子吸收光谱法检测","authors":"Gilberto S. Coelho Junior , Vinicius P. Souza , Jan Kratzer , Jiří Dědina , Erico M.M. Flores","doi":"10.1016/j.sab.2024.107055","DOIUrl":null,"url":null,"abstract":"<div><div>Mercury determination in fish tissues by high-resolution continuum source atomic absorption spectrometry (HR-CS AAS) was performed using microwave-induced combustion (MIC) for sample preparation, and plasma-mediated vapor generation (PMVG) in a tubular dielectric barrier discharge (DBD) as a sample introduction system. The main goal was to develop a method for Hg determination using very low amount of reagents being at the same time virtually free of interferences. The operational parameters of the PMVG step in the DBD reactor were optimized as follows: 70 mL min<sup>−1</sup> Ar as the discharge gas, 20 mm electrode distance, primary voltage at 60 V and 50 μL as digested sample volume (in 0.1 mol L<sup>−1</sup> HNO<sub>3</sub> matrix). The effect of HNO<sub>3</sub> concentration on the PMVG efficiency was studied. No differences in signal intensity were observed for HNO<sub>3</sub> concentration up to 0.1 mol L<sup>−1</sup>, while a signal decrease by 25 % and 40 % was observed when the acid concentration increased to 1 and 5 mol L<sup>−1</sup>, respectively. Accuracy of the proposed method was evaluated using certified reference material (CRM) of dogfish liver (DOLT-4) and by Hg spikes on the sample prior to MIC. By using 500 mg of sample (or 250 mg of CRM) and 6 mL of 0.5 mol L<sup>−1</sup> HNO<sub>3</sub> as absorbing solution in MIC, recoveries of 104 % and 97 % were observed for spikes and CRM values, respectively. A limit of detection of 0.16 μg g<sup>−1</sup> Hg (160 pg Hg absolute) was obtained. A comparison of the results for Hg determination in the CRM by PMVG-AAS and chemical cold vapor generation inductively coupled plasma mass spectrometry (CVG-ICP-MS) was performed with no significant differences in the results. Finally, an efficient, low-cost and low-volume reagent consumption sample introduction system for Hg determination by AAS was successfully combined with the advantages of MIC and subsequent PMVG by DBD.</div></div>","PeriodicalId":21890,"journal":{"name":"Spectrochimica Acta Part B: Atomic Spectroscopy","volume":"221 ","pages":"Article 107055"},"PeriodicalIF":3.2000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Plasma-mediated mercury vapor generation after microwave-induced combustion of fish tissue with detection by atomic absorption spectrometry\",\"authors\":\"Gilberto S. Coelho Junior , Vinicius P. Souza , Jan Kratzer , Jiří Dědina , Erico M.M. Flores\",\"doi\":\"10.1016/j.sab.2024.107055\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Mercury determination in fish tissues by high-resolution continuum source atomic absorption spectrometry (HR-CS AAS) was performed using microwave-induced combustion (MIC) for sample preparation, and plasma-mediated vapor generation (PMVG) in a tubular dielectric barrier discharge (DBD) as a sample introduction system. The main goal was to develop a method for Hg determination using very low amount of reagents being at the same time virtually free of interferences. The operational parameters of the PMVG step in the DBD reactor were optimized as follows: 70 mL min<sup>−1</sup> Ar as the discharge gas, 20 mm electrode distance, primary voltage at 60 V and 50 μL as digested sample volume (in 0.1 mol L<sup>−1</sup> HNO<sub>3</sub> matrix). The effect of HNO<sub>3</sub> concentration on the PMVG efficiency was studied. No differences in signal intensity were observed for HNO<sub>3</sub> concentration up to 0.1 mol L<sup>−1</sup>, while a signal decrease by 25 % and 40 % was observed when the acid concentration increased to 1 and 5 mol L<sup>−1</sup>, respectively. Accuracy of the proposed method was evaluated using certified reference material (CRM) of dogfish liver (DOLT-4) and by Hg spikes on the sample prior to MIC. By using 500 mg of sample (or 250 mg of CRM) and 6 mL of 0.5 mol L<sup>−1</sup> HNO<sub>3</sub> as absorbing solution in MIC, recoveries of 104 % and 97 % were observed for spikes and CRM values, respectively. A limit of detection of 0.16 μg g<sup>−1</sup> Hg (160 pg Hg absolute) was obtained. A comparison of the results for Hg determination in the CRM by PMVG-AAS and chemical cold vapor generation inductively coupled plasma mass spectrometry (CVG-ICP-MS) was performed with no significant differences in the results. Finally, an efficient, low-cost and low-volume reagent consumption sample introduction system for Hg determination by AAS was successfully combined with the advantages of MIC and subsequent PMVG by DBD.</div></div>\",\"PeriodicalId\":21890,\"journal\":{\"name\":\"Spectrochimica Acta Part B: Atomic Spectroscopy\",\"volume\":\"221 \",\"pages\":\"Article 107055\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2024-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Spectrochimica Acta Part B: Atomic Spectroscopy\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0584854724002003\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"SPECTROSCOPY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part B: Atomic Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0584854724002003","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}
Plasma-mediated mercury vapor generation after microwave-induced combustion of fish tissue with detection by atomic absorption spectrometry
Mercury determination in fish tissues by high-resolution continuum source atomic absorption spectrometry (HR-CS AAS) was performed using microwave-induced combustion (MIC) for sample preparation, and plasma-mediated vapor generation (PMVG) in a tubular dielectric barrier discharge (DBD) as a sample introduction system. The main goal was to develop a method for Hg determination using very low amount of reagents being at the same time virtually free of interferences. The operational parameters of the PMVG step in the DBD reactor were optimized as follows: 70 mL min−1 Ar as the discharge gas, 20 mm electrode distance, primary voltage at 60 V and 50 μL as digested sample volume (in 0.1 mol L−1 HNO3 matrix). The effect of HNO3 concentration on the PMVG efficiency was studied. No differences in signal intensity were observed for HNO3 concentration up to 0.1 mol L−1, while a signal decrease by 25 % and 40 % was observed when the acid concentration increased to 1 and 5 mol L−1, respectively. Accuracy of the proposed method was evaluated using certified reference material (CRM) of dogfish liver (DOLT-4) and by Hg spikes on the sample prior to MIC. By using 500 mg of sample (or 250 mg of CRM) and 6 mL of 0.5 mol L−1 HNO3 as absorbing solution in MIC, recoveries of 104 % and 97 % were observed for spikes and CRM values, respectively. A limit of detection of 0.16 μg g−1 Hg (160 pg Hg absolute) was obtained. A comparison of the results for Hg determination in the CRM by PMVG-AAS and chemical cold vapor generation inductively coupled plasma mass spectrometry (CVG-ICP-MS) was performed with no significant differences in the results. Finally, an efficient, low-cost and low-volume reagent consumption sample introduction system for Hg determination by AAS was successfully combined with the advantages of MIC and subsequent PMVG by DBD.
期刊介绍:
Spectrochimica Acta Part B: Atomic Spectroscopy, is intended for the rapid publication of both original work and reviews in the following fields:
Atomic Emission (AES), Atomic Absorption (AAS) and Atomic Fluorescence (AFS) spectroscopy;
Mass Spectrometry (MS) for inorganic analysis covering Spark Source (SS-MS), Inductively Coupled Plasma (ICP-MS), Glow Discharge (GD-MS), and Secondary Ion Mass Spectrometry (SIMS).
Laser induced atomic spectroscopy for inorganic analysis, including non-linear optical laser spectroscopy, covering Laser Enhanced Ionization (LEI), Laser Induced Fluorescence (LIF), Resonance Ionization Spectroscopy (RIS) and Resonance Ionization Mass Spectrometry (RIMS); Laser Induced Breakdown Spectroscopy (LIBS); Cavity Ringdown Spectroscopy (CRDS), Laser Ablation Inductively Coupled Plasma Atomic Emission Spectroscopy (LA-ICP-AES) and Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS).
X-ray spectrometry, X-ray Optics and Microanalysis, including X-ray fluorescence spectrometry (XRF) and related techniques, in particular Total-reflection X-ray Fluorescence Spectrometry (TXRF), and Synchrotron Radiation-excited Total reflection XRF (SR-TXRF).
Manuscripts dealing with (i) fundamentals, (ii) methodology development, (iii)instrumentation, and (iv) applications, can be submitted for publication.