在Hyptis suaveolens（L.）Poit.根培养物中积累荚叶素：一种可用于临床抗癌药物的潜在天然木质素

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-19 DOI:10.1016/j.bcab.2024.103416

Sâmia Torres Silva , Rafael Marlon Alves de Assis , Adriane Duarte Coelho , Simony Carvalho Mendonça , Lurdeslaine Faria Texeira , Suzan Kelly Vilela Bertolucci , José Eduardo Brasil Pereira Pinto

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引用次数: 0

摘要

鬼臼毒素（PTOX）是一种天然的抗病毒、抗风湿和抗癌分子，但其化学合成成本高昂。本研究旨在评估用不同浓度的辅助素、维生素和肌醇处理的半枝莲（Hyptis suaveolens）根培养物的生长和 PTOX 积累情况。在补充了不同浓度的吲哚-3-丁酸（IBA）、1-萘乙酸（NAA）、维生素和肌醇的液体培养基中生长的根培养物被用来测量生物量产量和 PTOX 含量。在成功验证后，采用新开发的分析方法，通过高效液相色谱-二极管阵列检测（HPLC-DAD）对 PTOX 进行了定量。在添加了 1 mg L-1 IBA +0.5 mg L-1 NAA 的 MS 培养基中培养的根干重最大（248.76 mg），根中的 PTOX 浓度最高（179.97 μg g-1 根）。在补充了 1 mg L-1 IBA +0.5 mg L-1 NAA 的液体 MS 培养基中培养的根，其根生物量和 PTOX 含量的增幅最大。液体 MS 培养基中维生素和肌醇的充分平衡补充增加了根的干重和 PTOX 含量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Accumulation of podophyllotoxin in the root culture of Hyptis suaveolens (L.) Poit: A potential natural lignan for clinically useful anticancer drugs

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Accumulation of podophyllotoxin in the root culture of Hyptis suaveolens (L.) Poit: A potential natural lignan for clinically useful anticancer drugs

Podophyllotoxin (PTOX) is a natural antiviral, antirheumatic and anticancer molecule, but it is expensive to chemically synthesize. The present study aimed to evaluate growth and PTOX accumulation in root cultures of Hyptis suaveolens treated with different concentrations of auxins, vitamins and myo-inositol. Root cultures grown in liquid medium supplemented with different concentrations of indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA), vitamins and myo-inositol were used to measure biomass production and PTOX content. PTOX quantitation was performed via high-performance liquid chromatography with diode-array detection (HPLC-DAD) using a newly developed analytical method after successful validation. Root culture in MS medium supplemented with 1 mg L⁻¹ IBA +0.5 mg L⁻¹ NAA resulted in the highest root dry weight (248.76 mg) and the highest PTOX concentration in the roots (179.97 μg g⁻¹ root). The roots cultured in liquid MS medium supplemented with 1 mg L⁻¹ IBA +0.5 mg L⁻¹ NAA presented the greatest increase in root biomass and PTOX content. This adequate balance of vitamin and myo-inositol supplementation in liquid MS culture medium increased the root dry weight and PTOX content.

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.