两种来自长春花的新型杂多糖的结构特征及其免疫活性评估

IF 10.7 1区 化学 Q1 CHEMISTRY, APPLIED
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引用次数: 0

摘要

长春花是一种在中国民间医学中具有重要治疗价值的植物,含有大量次生代谢物。然而,初级代谢物,特别是可能在免疫疗法中发挥重要作用的多糖,受到的关注却很有限。本研究从蔷薇中分离出两种新型多糖,分别命名为 CRPS-1 和 CRPS-2。采用 HPSEC、HPLC、IR、GC-MS、1D NMR 和 2D NMR 等方法对 CRPS-1 和 CRPS-2 的结构进行了表征。经鉴定,CRPS-1 和 CRPS-2 均为均质杂多糖。此外,CRPS-2 的平均分子量低于 CRPS-1。CRPS-1 的主链由 1,3-α-L-Araf、1,5-α-L-Araf、1,3,5-α-L-Araf、1,3,4-α-L-Rhap、1,3-α-D-Galp、1,3,4-α-D-Galp、1,4-β-D-Manp 组成,侧链由 T-α-L-Araf、T-β-D-Manp 和 β-D-Glcp-(1 → 3)-α-D-Galp-(1 → 3)-α-L-Rhap-(1→) 组成。CRPS-2主要由具有复杂分支结构的1,3-α-D-Galp、1,3,4-α-D-Galp、1,6-β-D-Manp、1,5-α-L-Araf、1,3,5-α-L-Araf、1,3-α-L-Rhap和1,3,4-α-L-Fucp组成。此外,CRPS-2 还能显著增强 RAW264.7 细胞的增殖和吞噬能力以及细胞因子的分泌。它通过激活 MAPK/Akt/NF-κB 信号通路,显示出强大的免疫调节活性。总之,利用富含半乳糖的低分子量多糖在开发可提供健康益处的创新功能食品方面具有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structural characterization of two novel heteropolysaccharides from Catharanthus roseus and the evaluation of their immunological activities
Catharanthus roseus, a plant with significant therapeutic value in Chinese folk medicine, contain numerous secondary metabolites. However, the primary metabolites, specifically polysaccharides which might play an important role in immunotherapy, have received limited attention. In the present study, two novel polysaccharides, designated as CRPS-1 and CRPS-2, were isolated from C. roseus. The structures of CRPS-1 and CRPS-2 were characterized using a combination of HPSEC, HPLC, IR, GC‐MS, 1D NMR and 2D NMR. Both CRPS-1 and CRPS-2 were identified as homogeneous heteropolysaccharides. Additionally, the weight-average molecular weight of CRPS-2 was lower than that of CRPS-1. The backbone of CRPS-1 was composed of 1,3-α-L-Araf, 1,5-α-L-Araf, 1,3,5-α-L-Araf, 1,3,4-α-L-Rhap, 1,3-α-D-Galp, 1,3,4-α-D-Galp, 1,4-β-D-Manp, and side chains comprised of T-α-L-Araf, T-β-D-Manp, and β-D-Glcp-(1 → 3)-α-D-Galp-(1 → 3) -α-L-Rhap-(1→. CRPS-2 mainly consisted of 1,3-α-D-Galp, 1,3,4-α-D-Galp, 1,6-β-D-Manp, 1,5-α-L-Araf, 1,3,5-α-L-Araf, 1,3-α-L-Rhap and 1,3,4-α-L-Fucp with complex branching structures. Furthermore, CRPS-2 could significantly enhance proliferation and phagocytosis, as well as the secretion of cytokines in RAW264.7 cells. It demonstrated potent immunoregulatory activity by activating the MAPK/Akt/NF-κB signaling pathways. In summary, the utilization of galactose-enriched and low-molecular-weight polysaccharides exhibits great potential in the advancement of innovative functional foods that may provide health benefits.
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来源期刊
Carbohydrate Polymers
Carbohydrate Polymers 化学-高分子科学
CiteScore
22.40
自引率
8.00%
发文量
1286
审稿时长
47 days
期刊介绍: Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.
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