Madhunika Agrawal , A.K. Saxena , Satyam Kumar Agrawal
{"title":"虎杖精油在人类白血病 HL-60 细胞中发挥 caspase-3 介导的细胞凋亡作用","authors":"Madhunika Agrawal , A.K. Saxena , Satyam Kumar Agrawal","doi":"10.1016/j.sajb.2024.10.021","DOIUrl":null,"url":null,"abstract":"<div><div>The present study discusses the apoptotic potential of essential oil from whole plant of <em>Ocimum viride</em> (EOV) against human promyelocytic leukemia HL-60 cells. IC<sub>50</sub> value of EOV on HL-60 cells was 0.087, 0.0503, and 0.034 µl/ml respectively at 6, 12, and 24 h. Several parameters were used to access the role of EOV in inducing apoptosis in human HL-60 cancer cells, including nuclear binding fluoroprobes, electron micrographs, DNA laddering, and annexin V-FITC/PI affinity assay. Quantification of BrdU incorporation was done to determine the degree of DNA damage. It was further determined that EOV has an effect on the DNA cell cycle and mitochondria, which was observed in the form of a prominent subdivision of G<sub>0</sub> peaks in the analysis of the DNA cell cycle, as well as a reduction in the mitochondrial membrane potential. A higher level of cytosolic cytochrome-c was associated with these events. Bcl-2 depletion and increase in ROS were also analysed. Both initiator caspases namely caspase-9 and -8 activated caspase-3 which eventually leads to apoptosis. These effects revealed that EOV has apoptosis inducing potential towards HL-60 cells and can be a promising candidate for further anticancer studies.</div></div>","PeriodicalId":21919,"journal":{"name":"South African Journal of Botany","volume":"175 ","pages":"Pages 193-200"},"PeriodicalIF":2.7000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Essential oil from Ocimum viride exerts caspase-3 interceded apoptosis in human leukemia HL-60 cells\",\"authors\":\"Madhunika Agrawal , A.K. Saxena , Satyam Kumar Agrawal\",\"doi\":\"10.1016/j.sajb.2024.10.021\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The present study discusses the apoptotic potential of essential oil from whole plant of <em>Ocimum viride</em> (EOV) against human promyelocytic leukemia HL-60 cells. IC<sub>50</sub> value of EOV on HL-60 cells was 0.087, 0.0503, and 0.034 µl/ml respectively at 6, 12, and 24 h. Several parameters were used to access the role of EOV in inducing apoptosis in human HL-60 cancer cells, including nuclear binding fluoroprobes, electron micrographs, DNA laddering, and annexin V-FITC/PI affinity assay. Quantification of BrdU incorporation was done to determine the degree of DNA damage. It was further determined that EOV has an effect on the DNA cell cycle and mitochondria, which was observed in the form of a prominent subdivision of G<sub>0</sub> peaks in the analysis of the DNA cell cycle, as well as a reduction in the mitochondrial membrane potential. A higher level of cytosolic cytochrome-c was associated with these events. Bcl-2 depletion and increase in ROS were also analysed. Both initiator caspases namely caspase-9 and -8 activated caspase-3 which eventually leads to apoptosis. These effects revealed that EOV has apoptosis inducing potential towards HL-60 cells and can be a promising candidate for further anticancer studies.</div></div>\",\"PeriodicalId\":21919,\"journal\":{\"name\":\"South African Journal of Botany\",\"volume\":\"175 \",\"pages\":\"Pages 193-200\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-10-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"South African Journal of Botany\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0254629924006525\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"South African Journal of Botany","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0254629924006525","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Essential oil from Ocimum viride exerts caspase-3 interceded apoptosis in human leukemia HL-60 cells
The present study discusses the apoptotic potential of essential oil from whole plant of Ocimum viride (EOV) against human promyelocytic leukemia HL-60 cells. IC50 value of EOV on HL-60 cells was 0.087, 0.0503, and 0.034 µl/ml respectively at 6, 12, and 24 h. Several parameters were used to access the role of EOV in inducing apoptosis in human HL-60 cancer cells, including nuclear binding fluoroprobes, electron micrographs, DNA laddering, and annexin V-FITC/PI affinity assay. Quantification of BrdU incorporation was done to determine the degree of DNA damage. It was further determined that EOV has an effect on the DNA cell cycle and mitochondria, which was observed in the form of a prominent subdivision of G0 peaks in the analysis of the DNA cell cycle, as well as a reduction in the mitochondrial membrane potential. A higher level of cytosolic cytochrome-c was associated with these events. Bcl-2 depletion and increase in ROS were also analysed. Both initiator caspases namely caspase-9 and -8 activated caspase-3 which eventually leads to apoptosis. These effects revealed that EOV has apoptosis inducing potential towards HL-60 cells and can be a promising candidate for further anticancer studies.
期刊介绍:
The South African Journal of Botany publishes original papers that deal with the classification, biodiversity, morphology, physiology, molecular biology, ecology, biotechnology, ethnobotany and other botanically related aspects of species that are of importance to southern Africa. Manuscripts dealing with significant new findings on other species of the world and general botanical principles will also be considered and are encouraged.