甜高粱 SWEET 基因家族的全基因组鉴定和表达分析以及 SdSWEET01 在糖运输中的作用

IF 5.4 Q1 PLANT SCIENCES
Chengcai Pan , Yu Wang , Yiyin Ji , Yang Zhou , Xingyu Jiang
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引用次数: 0

摘要

SWEET 糖转运体在植物的生长发育过程中发挥着重要作用。本研究从甜高粱(Sorghum dochna)中鉴定了 18 个 SWEET 基因,这些基因编码的蛋白质具有 231-336 个氨基酸,分子量在 25.15 至 35.69 kDa 之间,等电点在 6.41 至 9.69 之间。系统发生分析将这些蛋白质分为四个不同的亚群。对空间表达模式的研究表明,SdSWEET 基因的表达具有组织特异性。此外,还观察到它们参与了对各种非生物胁迫(包括冷、热、干旱和盐度)的响应。酵母互补试验证实,位于质膜上的 SdSWEET01 可选择性地转运葡萄糖、蔗糖和半乳糖,而不转运果糖。与野生型植物相比,表达 SdSWEET01 的转基因拟南芥表现出更强的糖吸收能力,从而提高了在高糖条件下的敏感性和生长抑制作用。该研究提供了 SdSWEET 基因的详细功能特征,并强调了 SdSWEET01 在调节糖转运中的关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome-wide identification and expression analysis of the SWEET gene family in sweet sorghum (Sorghum dochna) and the role of SdSWEET01 in sugar transport
The SWEET sugar transporter plays a fundamental role in plant growth and development. In this study, 18 SWEET genes were identified from sweet sorghum (Sorghum dochna), encoding proteins with 231–336 amino acids, molecular weights from 25.15 to 35.69 kDa, and isoelectric points ranging between 6.41 and 9.69. Phylogenetic analysis categorized these proteins into four distinct subgroups. Examination of spatial expression patterns demonstrated that SdSWEET genes were expressed in a tissue-specific manner. Furthermore, their involvement in responses to various abiotic stresses, including cold, heat, drought, and salinity was observed. A yeast complementation assay verified that SdSWEET01, located on the plasma membrane, selectively transported glucose, sucrose, and galactose, while excluding fructose. Transgenic Arabidopsis expressing SdSWEET01 exhibited enhanced sugar absorption compared to wild-type plants, resulting in increased sensitivity and growth inhibition under high-sugar conditions. The study provides a detailed functional characterization of SdSWEET genes and emphasizes the critical role of SdSWEET01 in regulating sugar transport.
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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