{"title":"甜高粱 SWEET 基因家族的全基因组鉴定和表达分析以及 SdSWEET01 在糖运输中的作用","authors":"","doi":"10.1016/j.cpb.2024.100405","DOIUrl":null,"url":null,"abstract":"<div><div>The SWEET sugar transporter plays a fundamental role in plant growth and development. In this study, 18 <em>SWEET</em> genes were identified from sweet sorghum (<em>Sorghum dochna</em>), encoding proteins with 231–336 amino acids, molecular weights from 25.15 to 35.69 kDa, and isoelectric points ranging between 6.41 and 9.69. Phylogenetic analysis categorized these proteins into four distinct subgroups. Examination of spatial expression patterns demonstrated that <em>SdSWEET</em> genes were expressed in a tissue-specific manner. Furthermore, their involvement in responses to various abiotic stresses, including cold, heat, drought, and salinity was observed. A yeast complementation assay verified that SdSWEET01, located on the plasma membrane, selectively transported glucose, sucrose, and galactose, while excluding fructose. Transgenic <em>Arabidopsis</em> expressing <em>SdSWEET01</em> exhibited enhanced sugar absorption compared to wild-type plants, resulting in increased sensitivity and growth inhibition under high-sugar conditions. The study provides a detailed functional characterization of <em>SdSWEET</em> genes and emphasizes the critical role of SdSWEET01 in regulating sugar transport.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genome-wide identification and expression analysis of the SWEET gene family in sweet sorghum (Sorghum dochna) and the role of SdSWEET01 in sugar transport\",\"authors\":\"\",\"doi\":\"10.1016/j.cpb.2024.100405\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The SWEET sugar transporter plays a fundamental role in plant growth and development. In this study, 18 <em>SWEET</em> genes were identified from sweet sorghum (<em>Sorghum dochna</em>), encoding proteins with 231–336 amino acids, molecular weights from 25.15 to 35.69 kDa, and isoelectric points ranging between 6.41 and 9.69. Phylogenetic analysis categorized these proteins into four distinct subgroups. Examination of spatial expression patterns demonstrated that <em>SdSWEET</em> genes were expressed in a tissue-specific manner. Furthermore, their involvement in responses to various abiotic stresses, including cold, heat, drought, and salinity was observed. A yeast complementation assay verified that SdSWEET01, located on the plasma membrane, selectively transported glucose, sucrose, and galactose, while excluding fructose. Transgenic <em>Arabidopsis</em> expressing <em>SdSWEET01</em> exhibited enhanced sugar absorption compared to wild-type plants, resulting in increased sensitivity and growth inhibition under high-sugar conditions. The study provides a detailed functional characterization of <em>SdSWEET</em> genes and emphasizes the critical role of SdSWEET01 in regulating sugar transport.</div></div>\",\"PeriodicalId\":38090,\"journal\":{\"name\":\"Current Plant Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.4000,\"publicationDate\":\"2024-10-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Plant Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214662824000872\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Plant Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214662824000872","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Genome-wide identification and expression analysis of the SWEET gene family in sweet sorghum (Sorghum dochna) and the role of SdSWEET01 in sugar transport
The SWEET sugar transporter plays a fundamental role in plant growth and development. In this study, 18 SWEET genes were identified from sweet sorghum (Sorghum dochna), encoding proteins with 231–336 amino acids, molecular weights from 25.15 to 35.69 kDa, and isoelectric points ranging between 6.41 and 9.69. Phylogenetic analysis categorized these proteins into four distinct subgroups. Examination of spatial expression patterns demonstrated that SdSWEET genes were expressed in a tissue-specific manner. Furthermore, their involvement in responses to various abiotic stresses, including cold, heat, drought, and salinity was observed. A yeast complementation assay verified that SdSWEET01, located on the plasma membrane, selectively transported glucose, sucrose, and galactose, while excluding fructose. Transgenic Arabidopsis expressing SdSWEET01 exhibited enhanced sugar absorption compared to wild-type plants, resulting in increased sensitivity and growth inhibition under high-sugar conditions. The study provides a detailed functional characterization of SdSWEET genes and emphasizes the critical role of SdSWEET01 in regulating sugar transport.
期刊介绍:
Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.