二甲基三硫醚可减少采后炭疽病并提高芒果品质,是防治球孢子菌的潜在分子机制

IF 4.2 1区 农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Lihua Tang , Ruyun Qin , Suiping Huang , Xiaolin Chen , Tangxun Guo , Tom Hsiang , Qili Li
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引用次数: 0

摘要

芒果炭疽病主要由球孢子菌(Colletotrichum gloeosporioides)引起,是芒果在贮藏和运输过程中的主要破坏性采后病害。二甲基三硫醚(DMTS)是存在于某些微生物或植物中的一种有机挥发物,可在体外抑制球孢子菌的生长,但其对芒果炭疽病的影响及其分子作用机制尚未得到很好的描述。在本研究中,DMTS 对芒果中的 Colletotrichum spp.的 EC50 主要介于 2.3 至 20.0 μL/L 之间。在体内,使用 20 μL/L 的 DMTS 熏蒸 24 小时,或使用 80 μL/L 的 DMTS 熏蒸 3 小时或 6 小时,可有效降低采后芒果炭疽病(天然接种物)的严重程度,处理 10 天后观察到的抑制效果分别为 61.7%、65.7% 和 69.4%。此外,与未处理的对照组相比,芒果皮和果肉中均未检测到 DMTS 残留物,果实的整体质量也有所改善,可溶性固形物、总糖、维生素 c 和 β-胡萝卜素含量更高,可滴定酸度更低。基因表达分析表明,DMTS能显著抑制麦角甾醇生物合成相关基因Cgerg6和Cgerg11的表达。这两个基因的基因敲除突变体对 DMTS 的敏感性降低。基因原位互补后,互补转化体对DMTS的敏感性恢复到亲本菌株的水平。因此,我们认为 Cgerg6 和 Cgerg11 基因参与了 DMTS 抗真菌活性的相互作用。这是首次研究证明 DMTS 对芒果采后炭疽病有控制作用,从而降低了病害严重程度,提高了果实品质。转化体研究还揭示了 DMTS 抗真菌活性的一些潜在分子机制,这可能会改善芒果采后炭疽病的管理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dimethyl trisulfide reduces postharvest anthracnose and enhances mango quality, and a potential molecular mechanism against Colletotrichum gloeosporioides

Dimethyl trisulfide reduces postharvest anthracnose and enhances mango quality, and a potential molecular mechanism against Colletotrichum gloeosporioides
Mango anthracnose, mainly caused by Colletotrichum gloeosporioides, is the major destructive postharvest disease of mango during storage and transport. Dimethyl trisulfide (DMTS), an organic volatile found in some microorganisms or plants, inhibited growth of C. gloeosporioides in vitro, but its effects on mango anthracnose and its molecular mechanisms of action have not been well characterized. In this study, the EC50 of DMTS against Colletotrichum spp. from mango mainly ranged from 2.3 to 20.0 μL/L. In vivo, the fumigation rates of 20 μL/L of DMTS for 24 h, or 80 μL/L for 3 h or 6 h could effectively reduce severity of anthracnose (natural inoculum) on postharvest mangoes with inhibitory effects of 61.7 %, 65.7 %, and 69.4 %, respectively, as observed 10 days after treatment. Furthermore, there was no detectable DMTS residue in mango skin or flesh, and an overall improvement in the quality of the fruit with higher soluble solids, total sugars, vitamin c, and β-carotene, and lower titratable acidity than the non-treated control. In addition, DMTS could significantly reduce ergosterol content in mycelia of C. gloeosporioides, and gene expression analysis showed DMTS significantly suppressed expression of ergosterol biosynthesis-related genes Cgerg6 and Cgerg11 after mycelia were exposed to DMTS. Knock-out mutants for each of these two genes showed reduced sensitivity to DMTS. After gene complementation in situ, the sensitivity of complementary transformants to DMTS was restored to that of the parental strain. Therefore, we concluded that the genes Cgerg6 and Cgerg11 are involved in an interaction with the antifungal activity of DMTS. This is the first study to demonstrate a control effect of DMTS on mango postharvest anthracnose resulting in reduced disease severity and enhanced fruit quality. Transformant studies also revealed some potential molecular mechanisms of the antifungal activity of DMTS that may lead to improved management of mango postharvest anthracnose.
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来源期刊
CiteScore
7.00
自引率
8.50%
发文量
238
审稿时长
4.2 months
期刊介绍: Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.
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