Ajuga parviflora Benth.的质量控制方法（UPLC-PDA）及其对分化脂肪前体细胞的抗脂肪生成作用

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine Pub Date : 2024-09-26 DOI:10.1016/j.hermed.2024.100950

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引用次数: 0

摘要

导言：Ajuga parviflora 传统上用于治疗发烧、糖尿病和消化系统疾病。目前，超高效液相色谱-光电二极管阵列（UPLC-PDA）法测定西洋接骨木中的矢车菊酮 C、西洋接骨木酮和香草酸，旨在从质量控制的角度开发和验证一种方法。方法根据国际协调理事会的指导原则，针对目标化合物开发并验证了 UPLC-PDA 方法。验证后的方法用于测定茜草提取物（乙醇：EtOH、50% EtOH 和水；叶和根）中的标记化合物。此外，还使用 UPLC-ELSD 测定了样品中的游离糖。此外，还检测了西番莲提取物对 3T3-L1 小鼠前脂肪细胞系的抗致脂作用。将细胞置于不同剂量的叶和根提取物中，评估脂质积累的程度。此外，在暴露于过氧化氢之前用不同的提取物处理细胞，然后评估A. parviflora 处理对氧化应激、细胞存活和胰岛素敏感性的影响。在所有样品中都发现了这些化合物。从生物学角度看，A. parviflora 的叶和根提取物通过降低细胞内脂质的积累，以剂量依赖的方式大大抑制了脂肪的生成。结论研究结果表明，叶片乙醇提取物具有强效的抗脂肪生成特性，UPLC-PDA 将成为一种可重复的方法，用于评估欧芹及其衍生产品的质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Quality Control Method (UPLC-PDA) of Ajuga parviflora Benth. and Its Antiadipogenic Effect on Differentiated Preadipocytes

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Quality Control Method (UPLC-PDA) of Ajuga parviflora Benth. and Its Antiadipogenic Effect on Differentiated Preadipocytes

Introduction

Ajuga parviflora is traditionally used for fever, diabetes, and digestive problems. Currently, Ultraperformance liquid chromatography-Photodiode array (UPLC-PDA) method to determine ajugasterone C, cyasterone, and vanillic acid in A. parviflora was focused to develop and validate a method for quality control perspective. Further, the evaluation of antiadipogenic and antidiabetic potential of A. parviflora was also targeted.

Methods

UPLC-PDA method was developed and validated for targeted compounds as per International Council on Harmonisation guidelines. The validated method was used to determine marker compounds in A. parviflora extracts (Ethanol: EtOH, 50% EtOH, and water; leaves and roots). Further, UPLC-ELSD was used to determine free sugars in samples. Moreover, the antiadipogenic effect of A. parviflora extracts was examined on 3T3-L1 murine preadipocytes cell line. Cells were subjected to various dosages of leaves and root extracts, and the extent of lipid accumulation was evaluated. Furthermore, cells were treated with different extracts prior to hydrogen peroxide exposure, and then the effects of A. parviflora treatments on oxidative stress, cell survival, and insulin sensitisation were assessed.

Results

The validated UPLC-PDA method was found reproducible to determine ajugasterone C, cyasterone, and vanillic acid. These compounds were found in all the samples. Biologically, leaves and roots extract of A. parviflora drastically suppressed adipogenesis by lowering intracellular lipid accumulation in dose-dependent manner. They improved insulin sensitivity by promoting glucose uptake and protected cellular health from oxidative damage by reducing reactive oxygen species generation and reversing apoptosis.

Conclusions

Findings suggested that ethanol extract of leaves exhibited potent antiadipogenic properties and UPLC-PDA will be a reproducible method to assess quality of A. parviflora and its derived products.

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来源期刊

Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.