过表达小麦 C2H2 锌指蛋白转录因子 TaZAT8-5B 可增强拟南芥的耐旱性和根系生长。

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-10-28 DOI:10.1007/s00425-024-04559-z
Lulu Chen, Run Wang, Xiaoqing Hu, Dan Wang, Yuexia Wang, Ruili Xue, Mingzhu Wu, Hua Li
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引用次数: 0

摘要

主要结论TaZAT8-5B是一种C2H2锌指蛋白转录因子,对转基因拟南芥的耐旱性有正向调节作用。它通过植物生长素信号通路中的 Aux/IAA-ARF 模块促进根系在干旱胁迫下的生长。C2H2 锌指蛋白(C2H2-ZFPs)是植物中最大的转录因子家族,但相对来说尚未被开发。这一点在小麦中尤为明显,只有少数 C2H2-ZFP 基因的功能得到了证实。在这项研究中,我们发现了一个新的 C2H2-ZFP 基因 TaZAT8-5B。该基因在根和花中的表达量很高,并在热胁迫、干旱胁迫和盐胁迫下被显著诱导。在干旱胁迫下,拟南芥过量表达 TaZAT8-5B 会导致叶片中脯氨酸含量和超氧化物歧化酶(SOD)活性增加。它还能减少气孔开度和水分流失,同时诱导 P5CS1、RD29A 和 DREB1A 的表达。因此,它减轻了干旱胁迫诱导的丙二醛(MDA)积累,提高了耐旱性。此外,TaZAT8-5B 还能促进甘露醇胁迫下侧根的萌发,并增强长期干旱胁迫下侧根和主根的生长。此外,TaZAT8-5B 还受到吲哚-3-乙酸(IAA)的诱导。在干旱胁迫下过表达 TaZAT8-5B 能显著抑制辅助素信号负调控基因 IAA12 和 IAA14 的表达。相反,与野生型(WT)植株相比,TaZAT8-5B过表达植株中IAA14和IAA12的下游基因(ARF7、LBD16、LBD18和CDKA1)上调。这些发现表明,在干旱胁迫下,TaZAT8-5B通过辅助素信号通路中的Aux/IAA-ARF模块调控根的生长和发育。总之,本研究阐明了TaZAT8-5B在增强耐旱性中的作用及其通过辅助素信号通路参与根系生长发育的过程。这些发现为TaZAT8-5B同源基因的功能分析,尤其是禾本科植物同源基因的功能分析提供了新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Overexpression of wheat C2H2 zinc finger protein transcription factor TaZAT8-5B enhances drought tolerance and root growth in Arabidopsis thaliana.

Main conclusion: TaZAT8-5B, a C2H2 zinc finger protein transcription factor, positively regulates drought tolerance in transgenic Arabidopsis. It promotes root growth under drought stress via the Aux/IAA-ARF module in the auxin signaling pathway. C2H2 zinc finger proteins (C2H2-ZFPs) represent the largest but relatively unexplored family of transcription factors in plants. This is particularly evident in wheat, where the functions of only a few C2H2-ZFP genes have been confirmed. In this study, we identified a novel C2H2-ZFP gene, TaZAT8-5B. This gene shows high expression in roots and flowers and is significantly induced by heat, drought, and salt stress. Under drought stress, overexpressing TaZAT8-5B in Arabidopsis resulted in increased proline content and superoxide dismutase (SOD) activity in leaves. It also led to reduced stomatal aperture and water loss, while inducing the expression of P5CS1, RD29A, and DREB1A. Consequently, it alleviated drought stress-induced malondialdehyde (MDA) accumulation and improved drought tolerance. Additionally, TaZAT8-5B promoted lateral root initiation under mannitol stress and enhanced both lateral and primary root growth under long-term drought stress. Moreover, TaZAT8-5B was induced by indole-3-acetic acid (IAA). Overexpressing TaZAT8-5B under drought stress significantly inhibited the expression of auxin signaling negative regulatory genes IAA12 and IAA14. Conversely, downstream genes (ARF7, LBD16, LBD18, and CDKA1) of IAA14 and IAA12 were upregulated in TaZAT8-5B overexpressing plants compared to wild-type (WT) plants. These findings suggest that TaZAT8-5B regulates root growth and development under drought stress via the Aux/IAA-ARF module in the auxin signaling pathway. In summary, this study elucidates the role of TaZAT8-5B in enhancing drought tolerance and its involvement in root growth and development through the auxin signaling pathway. These findings offer new insights into the functional analysis of homologous genes of TaZAT8-5B, particularly in Gramineae species.

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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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