使用纸点和中空纤维膜对大容量样品进行电萃取:提高分析性能的参数和策略研究。

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Ricardo Mathias Orlando, Miloš Dvořák, Pavel Kubáň
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引用次数: 0

摘要

电萃取(EE)包含一系列样品制备方法,其有效性、选择性和效率受到分析物、样品和仪器条件的物理化学特性的显著影响。本文首次探讨了旨在提高最新电萃取方法萃取效率的各种策略,该方法利用纸点(PP)结合中空纤维(HF)(缩写为 PP-HF-EE)从水样中萃取各种阳离子和阴离子模型化合物。研究还通过实验探讨了搅拌、有机过滤器成分、PP 直径和 PP 品牌对萃取性能的影响,并证明所有这些因素都相当重要,尤其是在利用数字图像分析进行测定时。此外,这项研究还证明了使用 PP-HF-EE 同时萃取阳离子和阴离子的简便性和可行性,并提出了一种简单易行的方法,即通过基底对顶点聚焦来提高 PP 顶点上的分析物浓度。最后,利用毛细管电泳和紫外-可见检测器证明,对于 PP-HF-EE,当每个样品使用多个 PP 时,萃取效率和预浓缩因子对其他参数的依赖性较小,去甲替林和氟哌啶醇的信号增强值分别高达 111 和 339。本文介绍的所有发现和策略都是对未来方法开发研究的重要贡献,尤其是在利用 PP-HF-EE 和类似的 EE 方法方面。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Electroextraction of Large Volume Samples Using Paper Points Coupled With Hollow Fiber Membranes: Study of Parameters and Strategies to Enhance Analytical Performance.

Electroextraction (EE) encompasses a range of sample preparation methods whose effectiveness, selectivity, and efficiency are significantly influenced by the physical-chemical characteristics of analytes, samples, and instrumental conditions. This article explores, for the first time, various strategies aimed at enhancing the extraction efficiency of a recent approach of EE utilizing a paper point (PP) combined with a hollow fiber (HF) (abbreviated as PP-HF-EE) to extract various cationic and anionic model compounds from water samples. The study also explores, experimentally, the impact of agitation, organic filter composition, PP diameter, and PP brand on extraction performance, and proves that all these factors are quite important, especially when digital image analysis is utilized for determination. Furthermore, this work demonstrates the ease and feasibility of simultaneously extracting cations and anions using PP-HF-EE and proposes a straightforward method to enhance analyte concentration on the vertex of the PP through a base-to-vertex focusing. Lastly, it is demonstrated, using capillary electrophoresis coupled to a UV-Vis detector, that for PP-HF-EE, the extraction efficiency and pre-concentration factor are less dependent on other parameters when multiple PPs per sample are utilized, with signal enhancement values of up to 111 and 339 for nortriptyline and haloperidol, respectively. All the findings and strategies presented herein constitute significant contributions that can facilitate future research in method development, particularly in the utilization of PP-HF-EE and similar EE approaches.

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来源期刊
ELECTROPHORESIS
ELECTROPHORESIS 生物-分析化学
CiteScore
6.30
自引率
13.80%
发文量
244
审稿时长
1.9 months
期刊介绍: ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.
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