Danúbia Aparecida Costa Nobre , Francisco Charles dos Santos Silva , João Filipe Rodrigues Guimarães , Cleberson Ribeiro , Valter Arthur , Carlos Sigueyuki Sediyama
{"title":"用伽马射线照射大豆种子可提高遗传变异性。","authors":"Danúbia Aparecida Costa Nobre , Francisco Charles dos Santos Silva , João Filipe Rodrigues Guimarães , Cleberson Ribeiro , Valter Arthur , Carlos Sigueyuki Sediyama","doi":"10.1016/j.apradiso.2024.111563","DOIUrl":null,"url":null,"abstract":"<div><div>The increase of genetic variability by the appearance of new genes of agronomic interest may be favored by the use of gamma radiation. The objective of this study was to evaluate different doses of gamma irradiation on dry seeds of VX04-5692 soybean line, aiming to increase the genetic variability and, with this, the identification of possible mutant plants. The doses of 0, 50, 150 and 250 Gy of gamma radiation were applied from a<sup>60</sup>Co source. The newly irradiated seeds were sown in the field, giving rise to the M1 cycle. Selected plants originated the M2 cycle. The number of seedlings was counted on the 21st day after sowing. Ten plants of each row were identified and evaluated for the various agronomic characteristics and for chemical composition. The data were submitted to analysis of variance. The F test was applied and the results were presented by boxplots and biplot (canonical variables). There was effect of gamma radiation doses at plant height at full bloom and maturity, number of nodes, pods with one seed and seeds per pod. The use of gamma radiation increases the variability in soybean, with consequent increase in the probabilities of identification of new mutants and gains in the chemical composition, useful for breeding programs that aim at better agronomic performance and gains in oil and protein contents. More satisfactory results in the generation of variability are obtained by the application of gamma radiation on soybean dry seeds between 50 and 150 Gy.</div></div>","PeriodicalId":8096,"journal":{"name":"Applied Radiation and Isotopes","volume":"215 ","pages":"Article 111563"},"PeriodicalIF":1.6000,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic variability increase by gamma irradiation of soybean seeds\",\"authors\":\"Danúbia Aparecida Costa Nobre , Francisco Charles dos Santos Silva , João Filipe Rodrigues Guimarães , Cleberson Ribeiro , Valter Arthur , Carlos Sigueyuki Sediyama\",\"doi\":\"10.1016/j.apradiso.2024.111563\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The increase of genetic variability by the appearance of new genes of agronomic interest may be favored by the use of gamma radiation. The objective of this study was to evaluate different doses of gamma irradiation on dry seeds of VX04-5692 soybean line, aiming to increase the genetic variability and, with this, the identification of possible mutant plants. The doses of 0, 50, 150 and 250 Gy of gamma radiation were applied from a<sup>60</sup>Co source. The newly irradiated seeds were sown in the field, giving rise to the M1 cycle. Selected plants originated the M2 cycle. The number of seedlings was counted on the 21st day after sowing. Ten plants of each row were identified and evaluated for the various agronomic characteristics and for chemical composition. The data were submitted to analysis of variance. The F test was applied and the results were presented by boxplots and biplot (canonical variables). There was effect of gamma radiation doses at plant height at full bloom and maturity, number of nodes, pods with one seed and seeds per pod. The use of gamma radiation increases the variability in soybean, with consequent increase in the probabilities of identification of new mutants and gains in the chemical composition, useful for breeding programs that aim at better agronomic performance and gains in oil and protein contents. More satisfactory results in the generation of variability are obtained by the application of gamma radiation on soybean dry seeds between 50 and 150 Gy.</div></div>\",\"PeriodicalId\":8096,\"journal\":{\"name\":\"Applied Radiation and Isotopes\",\"volume\":\"215 \",\"pages\":\"Article 111563\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2024-10-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Radiation and Isotopes\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0969804324003919\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, INORGANIC & NUCLEAR\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Radiation and Isotopes","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0969804324003919","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
Genetic variability increase by gamma irradiation of soybean seeds
The increase of genetic variability by the appearance of new genes of agronomic interest may be favored by the use of gamma radiation. The objective of this study was to evaluate different doses of gamma irradiation on dry seeds of VX04-5692 soybean line, aiming to increase the genetic variability and, with this, the identification of possible mutant plants. The doses of 0, 50, 150 and 250 Gy of gamma radiation were applied from a60Co source. The newly irradiated seeds were sown in the field, giving rise to the M1 cycle. Selected plants originated the M2 cycle. The number of seedlings was counted on the 21st day after sowing. Ten plants of each row were identified and evaluated for the various agronomic characteristics and for chemical composition. The data were submitted to analysis of variance. The F test was applied and the results were presented by boxplots and biplot (canonical variables). There was effect of gamma radiation doses at plant height at full bloom and maturity, number of nodes, pods with one seed and seeds per pod. The use of gamma radiation increases the variability in soybean, with consequent increase in the probabilities of identification of new mutants and gains in the chemical composition, useful for breeding programs that aim at better agronomic performance and gains in oil and protein contents. More satisfactory results in the generation of variability are obtained by the application of gamma radiation on soybean dry seeds between 50 and 150 Gy.
期刊介绍:
Applied Radiation and Isotopes provides a high quality medium for the publication of substantial, original and scientific and technological papers on the development and peaceful application of nuclear, radiation and radionuclide techniques in chemistry, physics, biochemistry, biology, medicine, security, engineering and in the earth, planetary and environmental sciences, all including dosimetry. Nuclear techniques are defined in the broadest sense and both experimental and theoretical papers are welcome. They include the development and use of α- and β-particles, X-rays and γ-rays, neutrons and other nuclear particles and radiations from all sources, including radionuclides, synchrotron sources, cyclotrons and reactors and from the natural environment.
The journal aims to publish papers with significance to an international audience, containing substantial novelty and scientific impact. The Editors reserve the rights to reject, with or without external review, papers that do not meet these criteria.
Papers dealing with radiation processing, i.e., where radiation is used to bring about a biological, chemical or physical change in a material, should be directed to our sister journal Radiation Physics and Chemistry.