VGSC 中的 V410L kdr 等位基因使埃及伊蚊(L. )的城市蚊子种群对氯菊酯产生更高水平的野外抗药性。

IF 3.8 1区 农林科学 Q1 AGRONOMY
Jonathan R Hernandez, Han-Jung Lee, Maximea E Vigilant, Scott Crawford, Patricia V Pietrantonio
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引用次数: 0

摘要

背景:埃及伊蚊的雌蚊传播新出现的虫媒病毒,包括寨卡、登革热、黄热病和基孔肯雅热。控制这些成蚊主要依靠合成杀虫剂,包括拟除虫菊酯。然而,杀虫剂抗药性在人群中的发展对病媒控制构成了巨大挑战,特别是除虫菊酯的靶标电压门控钠通道(VGSC)的抗药性(kdr)突变。本研究调查了以拟除虫菊酯为基础的杀虫剂普曼诺对埃及蚁的田间药效,评估了三种常见的 kdr 突变(V410L、V1016I、F1534C)在实际操作蚊虫控制情景下对蚊子存活率的影响,量化了杀虫剂在田间的施用量:结果:在进行实际灭蚊应用时,进行了现场笼试验(FCTs)。来自美国德克萨斯州哈里斯县六个作业区的雌蚊接触了用手持喷雾器喷洒的波马诺恩。使用气相色谱-质谱分析法(GC-MS)从 FCT 期间放置在田间的铝制小船上估算出蚊笼附近沉积的波马诺酮。记录死亡率,并对蚊子个体进行 kdr 突变基因分型。使用 probit 回归模型分析影响蚊子存活率的因素。随着与施药源路线的距离增加,珀曼酮的沉积量减少,导致具有三抗 kdr 基因型(LL/II/CC)的埃及雌蚊存活率增加。当 410 位点的 L 等位基因与其他 kdr 基因突变同时出现时,会显著提高抗性水平:这项研究将具有不同 kdr 基因型的蚊子的生存概率与它们在田间接受的杀虫剂数量联系起来。杀虫剂定量、控制效果结果和基因分型使我们能够根据经验确定基因型抗药性对田间病媒控制的影响。© 2024 化学工业协会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The V410L kdr allele in the VGSC confers higher levels of field resistance to permethrin in urban mosquito populations of Aedes aegypti (L.).

Background: Females of Aedes aegypti transmit emerging arboviruses including Zika, dengue, yellow fever, and chikungunya. Control of these adult mosquitoes heavily relies on synthetic insecticides, including pyrethroids. However, insecticide resistance development in populations poses a significant challenge to vector control, particularly from knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC), the target of pyrethroids. This study investigated the field efficacy of Permanone, a pyrethroid-based insecticide, against Ae. aegypti by assessing the impact of three common kdr mutations (V410L, V1016I, F1534C) on mosquito survival under a real operational mosquito control scenario, by quantifying the pesticide delivered in the field.

Results: Field cage tests (FCTs) were conducted while conducting a realistic mosquito control application. Female mosquitoes from six operational areas from Harris County, TX, USA were exposed to Permanone delivered with a handheld sprayer. Permanone deposited near the cages was estimated from aluminum boats placed in the field during FCTs using gas chromatography-mass spectrometry (GC-MS). Mortality rates were recorded, and individual mosquitoes were genotyped for kdr mutations. A probit regression model was used to analyze the factors influencing mosquito survivorship. As the distance from the application source route increased, the amount of Permanone deposited decreased, resulting in higher survivorship frequency of Ae. aegypti females with the triple-resistant kdr genotype (LL/II/CC). The L allele at the 410-site significantly contributed to an increased resistance level when co-occurring with other kdr mutations.

Conclusion: This study linked the survival probabilities of mosquitoes with different kdr genotypes, and the amount of pesticide they received in the field. Pesticide quantification, control efficacy results and genotyping allowed us to empirically determine the impact of genotypic resistance on vector control in the field. © 2024 Society of Chemical Industry.

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来源期刊
Pest Management Science
Pest Management Science 农林科学-昆虫学
CiteScore
7.90
自引率
9.80%
发文量
553
审稿时长
4.8 months
期刊介绍: Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.
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