Tuta absoluta 3-磷酸肌醇依赖性蛋白激酶-1在蛹蜕皮和成虫繁殖中的双重功能

IF 4.3 1区 农林科学 Q1 ENTOMOLOGY
Shu-Yan Yan, Jia-Hui Song, Wen-Jia Yang, Wan-xue Liu, Gui-Fen Zhang, Fang-Hao Wan, Nicolas Desneux, Yi-Bo Zhang
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引用次数: 0

摘要

磷酸肌醇依赖性激酶 PDK 是胰岛素信号通路的关键组成部分,在昆虫生长和发育的调控中发挥着重要作用。然而,PDK 基因在破坏性入侵番茄潜叶蝇(Tuta absoluta)中的功能意义仍然未知。在本研究中,我们鉴定了一个 PDK 基因(命名为 TaPDK),并分析了它在 T. absoluta 的蜕皮和繁殖过程中的生理作用。结果表明,TaPDK表达水平在蜕皮前的5天龄和6天龄蛹中升高,并在蜕皮后立即下降。TaPDK 主要在头部、中肠和腹部表达。当敲除 TaPDK 时,幼虫-蛹的蜕皮和雌性繁殖受到严重影响。向2日龄雌蛹注射TaPDK的dsRNA会导致31.29%的蛹发生严重的表型变化(角质层萎缩),最终导致死亡。TaPDK的敲除显著降低了20E滴度和几丁质含量,并下调了20E途径和几丁质代谢相关基因的表达水平。进一步研究发现,TaPDK的抑制会影响卵巢发育,导致受精率和孵化率明显降低。此外,TaPDK缺陷雌性的JH滴度和卵黄素含量降低,Vg、VgR和JH信号通路基因的表达也显著减少。总之,我们的研究深入揭示了PDK介导的蜕蛹和雌性繁殖调控。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Dual function of the Tuta absoluta 3-phosphoinositide-dependent protein kinase-1 in pupa ecdysis and adult reproduction

Dual function of the Tuta absoluta 3-phosphoinositide-dependent protein kinase-1 in pupa ecdysis and adult reproduction

Phosphoinositide-dependent kinase PDK, a key component of the insulin signaling pathway, plays an important role in the regulation of insect growth and development. However, the functional significance of the PDK gene in Tuta absoluta, a destructive invasive tomato leaf miner, remains unknown. In this study, we identified and characterized a PDK gene (designated TaPDK) and analyzed its physiological roles in molting and reproduction of T. absoluta. Our results showed that the TaPDK expression levels were elevated in the 5- and 6-day-old pupae before ecdysis and declined immediately after eclosion. TaPDK was predominantly expressed in the head, midgut and abdomen. When the TaPDK was knockdown, the larval-pupal molting and female reproduction were severely affected. Injecting dsRNA of TaPDK into 2-day-old female pupae resulted in severe phenotypic changes (cuticular shrinkage) in 31.29% of pupae, ultimately leading to death. Knockdown of TaPDK significantly reduced the 20E titer and chitin content and downregulated the expression levels of genes involved in the 20E pathway and chitin metabolism. Further investigation revealed that the suppression of TaPDK impaired ovarian development, resulting in significantly reduced fecundity and hatchability. In addition, the JH titer and vitellogenin content of TaPDK-deficient females were reduced, and the expression of Vg, VgR, and JH signaling pathway genes were significantly decreased. Collectively, our study provides a deep insight into the PDK-mediated regulation of pupal ecdysis and female reproduction in T. absoluta.

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来源期刊
Journal of Pest Science
Journal of Pest Science 生物-昆虫学
CiteScore
10.40
自引率
8.30%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.
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