抗击龋齿:利用香兰素和植物精油击败细菌分离物形成的生物膜:体外和体内方法

IF 3.4 Q2 PHARMACOLOGY & PHARMACY
Manar M. Ahmed, Nehal E. Yousef, Momen Askoura, Galal Yahya, Amira M. El-Ganiny
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引用次数: 0

摘要

背景生物膜形成细菌引起的感染与牙菌斑和龋齿有很大关系。本研究的目的是评估一些天然化合物在抑制和消除牙科感染中细菌分离物形成的生物膜方面的功效。结果从牙菌斑/龋齿中分离出细菌,并使用标准微生物测试和 16S rDNA 测序进行鉴定。分离出的细菌菌株包括金黄色葡萄球菌、表皮葡萄球菌、粪肠球菌、肺炎克雷伯菌和大肠埃希菌。抗生素敏感性是通过盘扩散法测定的,结果显示大多数分离菌株表现出较高的抗生素耐药性,61%的分离菌株具有多重耐药性。使用微孔板检测法研究了分离菌的生物膜形成能力。在 77 个细菌分离物中,17 个具有中等生物膜形成能力,22 个接近中等,34 个具有弱生物膜形成能力,4 个不产生生物膜。针对生物膜形成能力最强的分离物,对测试化合物(玫瑰和茉莉精油、蜂胶、香兰素和醋)的抗生物膜活性进行了评估。研究了受测物质单独或相互组合的体外抗生物膜能力,以评估它们阻止生物膜形成或破坏已形成的单种/多物种生物膜的能力。最后,在天然牙齿上对测试组合物的抗生物膜能力进行了体内外评估。我们的研究结果表明,香兰素与玫瑰油或茉莉油的组合在体外和体内模型中都表现出了良好的生物膜抑制和生物膜根除活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The battle against dental caries: defeating biofilm formed by bacterial isolates using vanillin and plant essential oils: in vitro and ex vivo approaches

Background

Infections caused by biofilm-forming bacteria have significantly linked to dental plaque and caries. The aim of this study is to assess efficacy of some natural compounds in inhibition and eradication of biofilm formed by bacterial isolates from dental infections.

Results

Bacterial isolates were recovered from dental plaque/caries and identified using standard microbiological tests and 16S rDNA sequencing. The isolated bacterial strains include Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Klebsiella pneumoniae, and Escherichia coli. The antibiotic susceptibility was determined by disk diffusion method and revealed that the majority of isolates showed high antibiotic resistance, and 61% of isolates were found to be multidrug resistant. The biofilm formation capacity of isolates was investigated using microtiter plate assay. Among the 77 bacterial isolates, seventeen showed moderate biofilm formation capacity, twenty-two showed near-moderate, thirty-four had weak biofilm-forming capacity, and four were non-biofilm producers. The antibiofilm activity of tested compounds (rose and jasmine oils, propolis, vanillin, and vinegar) was evaluated against isolates with highest biofilm-forming capacity. The in vitro antibiofilm ability of tested substances were investigated alone or in combination with each other to evaluate their ability to prevent biofilm formation or destroy preformed single-/multispecies biofilms. Finally, antibiofilm ability of tested combination was evaluated ex vivo on natural teeth. Our results showed that vanillin in combination with rose or jasmine oils showed promising biofilm inhibition and biofilm eradication activities in both the in vitro and ex vivo models.

Conclusions

Dental plaque and caries can be successfully prevented using combination of vanillin with rose or jasmine oils, and these compounds can be incorporated in new anticaries dental formulations.

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来源期刊
自引率
0.00%
发文量
44
审稿时长
23 weeks
期刊介绍: Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.
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