同种异体小鼠模型肺异体移植物移植后的远端肾脏和肝脏损伤

Transplantation proceedings Pub Date : 2024-11-01 Epub Date: 2024-10-24 DOI:10.1016/j.transproceed.2024.10.020
Marcin L Sander, Volker Eulenburg, Tatsuo Maeyashiki, Jae-Hwi Jang, Sarah D Müller, Sebastian N Stehr, Wolfgang Jungraithmayr, Tobias Piegeler
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引用次数: 0

摘要

背景:肺移植术后常见远端器官功能障碍,可能会对移植结果产生负面影响。以前曾证实局麻药罗哌卡因可减轻小鼠异体肺移植后的急性排斥反应。我们假设肺移植可能会导致肾脏和肝脏出现可检测到的分子损伤迹象,而罗哌卡因可能会减轻这种损伤:方法:在术后第 5 天采集接受同种异体正位单肺移植的 C57BL/6 小鼠的器官,并使用 Western 印迹和实时定量聚合酶链反应探针对 Src 蛋白酪氨酸激酶、STAT3 和 bax/bcl-2 进行分析。在冷缺血期间,同种异体移植物要么只用生理盐水冲洗,要么与罗哌卡因(1 µM)一起冲洗。一组未接受移植的动物作为基线对照:结果:与非移植动物相比,移植引起的异体刺激导致肺移植小鼠肾脏和肝脏中 Src 磷酸化和 STAT3 表达增加。作为细胞凋亡标志的 Bax/bcl-2 则不受移植影响。与移植肺的研究结果相反,添加罗哌卡因对远处器官的炎症指标没有影响:结论:观察到的炎症信号的增加首次揭示了肺移植后远处器官功能障碍的可能机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Remote Kidney and Liver Injury After Transplantation of Lung Allografts in an Allogeneic Mouse Model.

Background: Remote organ dysfunction is common after lung transplantation and might negatively affect the outcome. The local anesthetic ropivacaine was previously demonstrated to attenuate acute rejection after allogeneic lung transplantation in mice. We hypothesized that lung transplantation might result in detectable molecular signs of injury in kidneys and liver and that ropivacaine might attenuate this damage.

Methods: Organs from C57BL/6 mice undergoing allogeneic orthotopic single-lung transplantation were procured at postoperative day 5 and analyzed using Western blot and real-time quantitative polymerase chain reaction probing for Src protein tyrosine kinase, STAT3, and bax/bcl-2. During cold ischemia, the allograft had either been flushed with normal saline only or in combination with ropivacaine (1 µM). A nontransplanted group of animals served as the baseline controls.

Results: The allogeneic stimulus induced by transplantation led to an increase in Src-phosphorylation and STAT3-expression in the kidneys and livers of lung-transplanted mice compared to nontransplanted animals. Bax/bcl-2 as a marker of cellular apoptosis was not affected by the transplantation. In contrast to the findings in the transplanted lungs, the addition of ropivacaine did not have an effect on the examined markers of inflammation in the remote organs.

Conclusions: The observed increase in the inflammatory signaling provides first insight into a possible mechanism, by which remote organ dysfunction after lung transplantation might occur.

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