胶原蛋白纳米气泡是伊布替尼靶向控释的高效载体。

Sena Pişkin, Handan Sevim Akan, Canan Armutcu, Lokman Uzun
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引用次数: 0

摘要

纳米气泡旨在提高结构稳定性,并加强药物在目标部位的分布。由于纳米气泡体积小,因此可以从血液中有效渗透到所需区域。一般来说,气泡的结构是内部含有气体,外部由聚合物外壳包围。在本研究中,全氟戊烷被用作气体核心,而胶原蛋白因其生物可降解性和良好的生物相容性被用来形成外壳。在不同的 pH 值(5.5-7.4)和温度(4.0-40.0 °C)范围内,使用透析膜在 Franz 细胞中对制备的胶原蛋白纳米气泡进行了释放研究。在胶原蛋白纳米气泡的释放实验中观察到,在 pH 值为 7.4 的条件下,约 70% 的药物在 6 天内释放,而在超声波处理下爆炸后仅 24 小时内就达到了相同的释放率。同时,为了证明合成纳米气泡的有效性,还进行了细胞毒性研究。随着载药浓度和超声处理的增加,纳米气泡的细胞毒性活性与游离药物(伊布替尼)相似。此外,还进行了细胞培养研究,以HeLa细胞系作为软癌组织模型,评估纳米气泡的体外药物释放效率。总之,这些纳米气泡可作为携带活性药物治疗软组织肿瘤的有效替代品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Collagen nanobubbles as efficient carriers for targeted controlled release of ibrutinib.

Nanobubbles are designed to increase structural stability and enhance the distribution of the transported drug to the targeted site. They can efficiently penetrate the desired area from the bloodstream due to the small size of nanobubbles. In general, the structure of the bubbles contains a gas inside, surrounded by an outer polymeric shell. In this study, perfluoropentane was utilized as a gaseous core whereas collagen was used to form shells because of its biodegradability and excellent biocompatibility. The release studies of collagen nanobubbles prepared at several drug doses were carried out in a Franz cell using a dialysis membrane at different pH (5.5-7.4) and temperature (4.0-40.0 °C) ranges. In the release experiments with collagen nanobubbles, it was observed that approximately 70% of the drug was released within 6 days at pH 7.4 whereas the same releasing rate was achieved within only 24 h after exploding by ultrasound treatment. At the same time, a cytotoxicity study was carried out to demonstrate the effectiveness of the synthesized nanobubbles. With increasing drug loading concentration and ultrasound treatment, the cytotoxic activities of nanobubbles became similar to those of the free drug (ibrutinib). Furthermore, cell culture studies were performed to assess in vitro drug-releasing efficiencies of nanobubbles by using the HeLa cell line as a model of soft cancer tissue. In conclusion, these nanobubbles could be classified as an efficient alternative to carrying active agents for treating soft tissue tumors.

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来源期刊
Journal of materials chemistry. B
Journal of materials chemistry. B 化学科学, 工程与材料, 生命科学, 分析化学, 高分子组装与超分子结构, 高分子科学, 免疫生物学, 免疫学, 生化分析及生物传感, 组织工程学, 生物力学与组织工程学, 资源循环科学, 冶金与矿业, 生物医用高分子材料, 有机高分子材料, 金属材料的制备科学与跨学科应用基础, 金属材料, 样品前处理方法与技术, 有机分子功能材料化学, 有机化学
CiteScore
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