比较尿液中的 3-羟基苯并芘 (3-OHBaP) 和反式-7,8,9,10-四羟基-7,8,9,10-四氢苯并(a)芘 (TetraolBaP) 作为暴露于致癌物质 BaP 的生物标志物。

IF 4.5 2区医学 Q1 INFECTIOUS DISEASES

International journal of hygiene and environmental health Pub Date : 2024-10-23 DOI:10.1016/j.ijheh.2024.114476

Marie Marquès, Renaud Persoons

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引用次数: 0

摘要

简介：对暴露于致癌物质苯并（a）芘的生物监测通常基于尿液中 3-羟基苯并（a）芘（3-OHBaP）的测量，但其分析较为复杂，只能反映苯并（a）芘的解毒途径。四醇芘是新陈代谢活化途径产生的另一种芘代谢物，目前已经问世，但尚未在职业环境中进行研究，也未与 3-OHBaP 进行比较：对 118 名在铝冶炼行业工作的受试者进行了生物监测。每个受试者在工作周开始和结束时收集 3 份尿液样本。使用 LC-Fluorescence 和 GC-NCI-MS-MS 分析芘代谢物（1-羟基芘）和两种 BaP 生物标记物（3-OHBaP 和 TetraolBaP）：研究发现，工人的暴露程度很高，1-OHP 和 3-OHBaP 经常超过职业环境中的最高建议值。所有生物标志物的最高浓度都是在下班+16 小时时测得的，这说明皮肤接触和/或临时储存的情况比较突出。1-OHP 和 3-OHBaP 之间的相关性很强（r = 0.68-0.75），3-OHBaP 和 TetraolBaP 之间的相关性也很强（r = 0.67-0.78），1-OHP 和 TetraolBaP 之间的相关性一般（r = 0.59-0.76）。多环芳烃暴露量较低时，TetraolBaP 的含量较高，而暴露量较高时，TetraolBaP 的增加速度要慢得多，这表明生物活化途径逐渐饱和。研究发现，长期接触多环芳烃的工人的[3-OHBaP]/[TetraolBaP]比率明显较低。尿液中的 TetraolBaP 水平相当于 1-OHP （2.5 μg/L 或 1 μmol/mol 肌酐）或 3-OHBaP （0.4 nmol/mol 肌酐）的指导值，范围在 0.84 至 0.95 nmol/mol 肌酐之间：由致癌物质 BaP 代谢活化途径产生的四羟基苯并[BaP]被证明是一种具有诊断特异性和灵敏度的生物标志物，可用于确定受试者在不同环境（职业环境、环境）中暴露于多环芳烃的毒性，并评估健康风险。

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Comparison of urinary 3-hydroxybenzo(a)Pyrene (3-OHBaP) and trans-anti-7,8,9,10-tetrahydroxy-7,8,9,10-tetrahydrobenzo(a)Pyrene (TetraolBaP) as biomarkers of exposure to carcinogenic BaP

Introduction

Biomonitoring of exposure to carcinogenic Benzo(a)Pyrene is generally based on measurement of urinary 3-hydroxybenzo(a)pyrene (3-OHBaP), but its analysis is complex and only reflects the BaP detoxification pathway. TetraolBaP, another BaP metabolite resulting from the metabolic activation pathway, is now available but has not yet been studied in occupational settings or compared with 3-OHBaP.

Methods

Biomonitoring was carried out on 118 subjects working in the aluminium smelting industry. 3 urine samples were collected from each subject at the beginning and end of the working week. Pyrene metabolite (1-hydroxypyrene) and the two BaP biomarkers (3-OHBaP and TetraolBaP) were analysed using LC-Fluorescence and GC-NCI-MS-MS.

Results

The workers studied were found to be highly exposed, with 1-OHP and 3-OHBaP frequently exceeding maximum recommended values in occupational settings. Maximum concentrations were measured at end of shift+16h for all biomarkers, highlighting dermal exposure and/or temporary storage. Correlations were strong between 1-OHP and 3-OHBaP (r = 0.68–0.75) as well as between 3-OHBaP and TetraolBaP (r = 0.67–0.78), and moderate between 1-OHP and TetraolBaP (r = 0.59–0.76). While TetraolBaP levels were higher at low PAH exposures, TetraolBaP increased much more slowly at high exposures, indicating progressive saturation of the bioactivation pathway. The [3-OHBaP]/[TetraolBaP] ratio was found to be significantly lower in chronically exposed workers. Urinary TetraolBaP levels corresponding to 1-OHP (2.5 μg/L or 1 μmol/mol creatinine) or 3-OHBaP (0.4 nmol/mol creatinine) guidance values were found to range between 0.84 and 0.95 nmol/mol creatinine.

Conclusions

TetraolBaP, resulting from carcinogenic BaP's metabolic activation pathway, was shown to be a diagnostically specific and sensitive biomarker for determining subjects' toxic internal exposure to PAHs in different contexts (occupational settings, environment) and assessing health risks.

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来源期刊

International journal of hygiene and environmental health 医学-传染病学

CiteScore

11.50

自引率

5.00%

发文量

151

审稿时长

22 days

期刊介绍： The International Journal of Hygiene and Environmental Health serves as a multidisciplinary forum for original reports on exposure assessment and the reactions to and consequences of human exposure to the biological, chemical, and physical environment. Research reports, short communications, reviews, scientific comments, technical notes, and editorials will be peer-reviewed before acceptance for publication. Priority will be given to articles on epidemiological aspects of environmental toxicology, health risk assessments, susceptible (sub) populations, sanitation and clean water, human biomonitoring, environmental medicine, and public health aspects of exposure-related outcomes.