噬菌体对假体周围关节感染患者痤疮切迹杆菌的分离和抗生物膜活性

IF 3.8 3区 医学 Q2 VIROLOGY
Viruses-Basel Pub Date : 2024-10-10 DOI:10.3390/v16101592
Baixing Chen, Marco Chittò, Siyuan Tao, Jeroen Wagemans, Rob Lavigne, R Geoff Richards, Willem-Jan Metsemakers, T Fintan Moriarty
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引用次数: 0

摘要

背景:肩关节手术后感染,尤其是假体周围关节感染(PJI),治疗难度很大。痤疮棒状杆菌是39%至76%此类病例的致病病原体。本研究探讨了噬菌体疗法替代传统抗生素治疗此类感染的疗效:方法:以痤疮丙酸杆菌 ATCC 6919 为指示宿主,从人类皮肤中分离出九种具有溶菌活性的噬菌体。方法:以痤疮丙酸杆菌 ATCC 6919 为指示宿主,从人类皮肤中分离出了九种具有溶菌活性的噬菌体,并对这些噬菌体进行了单独或组合试验,以评估其宿主范围和对与痤疮丙酸杆菌相关的临床菌株的抗生物膜活性。对鸡尾酒噬菌体的广谱活性进行了优化,并针对钛盘上形成的生物膜进行了体外测试,以模拟假体环境:结果:分离出的噬菌体对一系列痤疮丙酸杆菌临床分离株具有杀菌活性。与未经处理的对照组相比,鸡尾酒噬菌体能明显减少痤疮丙酸杆菌 183、184 和 GG2A 株的细菌量(p < 0.05)。个别噬菌体,尤其是 CaJIE7 和 CaJIE3,也能显著减少特定菌株的细菌量。此外,噬菌体还明显破坏了生物膜结构,减少了生物膜生物量,证实了噬菌体疗法在针对生物膜相关感染方面的潜力:我们的临床前研究结果支持噬菌体疗法作为传统抗生素辅助疗法治疗骨科设备相关感染中痤疮丙酸杆菌感染的潜力。噬菌体具有破坏生物膜的能力,这对治疗与假体植入相关的感染尤其有益。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and Antibiofilm Activity of Bacteriophages against Cutibacterium acnes from Patients with Periprosthetic Joint Infection.

Background: Infections following shoulder surgery, particularly periprosthetic joint infection (PJI), are challenging to treat. Cutibacterium acnes is the causative pathogen in 39% to 76% of these cases. This study explores the efficacy of bacteriophage therapy as an alternative to conventional antibiotics for treating such infections.

Methods: Nine phages with lytic activity were isolated from the skin of humans using C. acnes ATCC 6919 as the indicator host. These phages were tested individually or in combination to assess host range and antibiofilm activity against clinical strains of C. acnes associated with PJIs. The phage cocktail was optimized for broad-spectrum activity and tested in vitro against biofilms formed on titanium discs to mimic the prosthetic environment.

Results: The isolated phages displayed lytic activity against a range of C. acnes clinical isolates. The phage cocktail significantly reduced the bacterial load of C. acnes strains 183, 184, and GG2A, as compared with untreated controls (p < 0.05). Individual phages, particularly CaJIE7 and CaJIE3, also demonstrated significant reductions in bacterial load with respect to specific strains. Moreover, phages notably disrupted the biofilm structure and reduced biofilm biomass, confirming the potential of phage therapy in targeting biofilm-associated infections.

Conclusions: Our preclinical findings support the potential of phage therapy as a viable adjunct to traditional antibiotics for treating C. acnes infections in orthopedic device-related infections. The ability of phages to disrupt biofilms may be particularly beneficial for managing infections associated with prosthetic implants.

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来源期刊
Viruses-Basel
Viruses-Basel VIROLOGY-
CiteScore
7.30
自引率
12.80%
发文量
2445
审稿时长
1 months
期刊介绍: Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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