虹鳟鱼(Oncorhynchus mykiss)不可预测的重复压力改变了对鱼类寄生虫的免疫反应。

IF 3.6 3区 生物学 Q1 BIOLOGY
Cyril Henard, Hanxi Li, Barbara F Nowak, Louise von Gersdorff Jørgensen
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引用次数: 0

摘要

由于养殖方式,养殖鱼类经常受到各种应激因素的影响,而这些应激因素对疾病爆发的影响尚不确定。这项研究评估了虹鳟鱼在受到纤毛虫Ichthyophthirius multifiliis(已知可导致淡水鱼白斑病)挑战时,不可预测的重复应激的影响。在接触病原体之前和之后,对鱼类进行了三种程序的随机轮换处理。感染后 7 天(dpi),评估鱼体内和鱼缸水中的寄生虫负荷,并分别调查鳃和脾脏的局部和全身免疫反应。鱼的死亡率一直记录到 12 dpi,感染组的鱼全部死亡。两个感染组之间的寄生虫负荷(鱼和鱼缸水)和感染严重程度没有统计学差异。免疫基因表达分析表明,鱼鳃和脾脏的免疫反应不同。在鳃中,启动了 T 辅助细胞 2 型免疫反应,而在脾脏中则观察到了 T 辅助细胞 1 型免疫反应。应激主要诱导鳃的免疫基因(cat-1、hep、il-10)上调,而脾的免疫基因(il-2、il-4/13a、il-8)下调。我们的研究结果表明,所采用的不可预测的重复应激方案并未损害鱼类的免疫系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Unpredictable Repeated Stress in Rainbow Trout (Oncorhynchus mykiss) Shifted the Immune Response against a Fish Parasite.

Farmed fish are regularly subjected to various stressors due to farming practices, and their effect in the context of a disease outbreak is uncertain. This research evaluated the effects of unpredictable repeated stress in rainbow trout challenged with the ciliate Ichthyophthirius multifiliis, known to cause white spot disease in freshwater fish. Before and after the pathogen exposure, fish were handled with a random rotation of three procedures. At 7 days post-infection (dpi), the parasite burden was evaluated in fish and in the tank's water, and the local and systemic immune responses were investigated in the gill and spleen, respectively. The fish mortality was recorded until 12 dpi, when all the fish from the infected groups died. There was no statistical difference in parasite burden (fish and tank's water) and infection severity between the two infected fish groups. The immune gene expression analysis suggested a differential immune response between the gill and the spleen. In gills, a T helper cell type 2 immune response was initiated, whereas in spleen, a T helper cell type 1 immune response was observed. The stress has induced mainly upregulations of immune genes in the gill (cat-1, hep, il-10) and downregulations in the spleen (il-2, il-4/13a, il-8). Our results suggested that the unpredictable repeated stress protocol employed did not impair the fish immune system.

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来源期刊
Biology-Basel
Biology-Basel Biological Science-Biological Science
CiteScore
5.70
自引率
4.80%
发文量
1618
审稿时长
11 weeks
期刊介绍: Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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