利用表面修饰的细菌纤维素和固定化噬菌体颗粒超灵敏电化学检测食品基质中的鼠伤寒沙门氏菌

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Wajid Hussain, Huan Wang, Xiaohan Yang, Muhammad Wajid Ullah, Jawad Hussain, Najeeb Ullah, Mazhar Ul-Islam, Mohamed F Awad, Shenqi Wang
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引用次数: 0

摘要

快速灵敏地检测食品基质中的鼠伤寒沙门氏菌对确保食品安全至关重要。本研究利用聚吡咯(Ppy)和还原氧化石墨烯(RGO)对细菌纤维素(BC)进行表面修饰,并进一步用固定的鼠伤寒沙门氏菌特异性噬菌体颗粒对其进行功能化,从而开发出一种超灵敏的电化学生物传感器。通过原位氧化聚合聚吡咯和还原氧化石墨烯(RGO),改变了具有超纤维和多孔结构的不饱和聚碳酸酯基底,从而形成了高导电性和柔韧性的生物界面。聚阳离子 Ppy 与带负电荷的噬菌体头盖之间的静电相互作用促进了噬菌体在这种复合材料上的固定,从而优化了噬菌体的定向并提高了细菌捕获效率。形态和化学特性分析证实了噬菌体的成功制造和固定。该生物传感器对磷酸盐缓冲盐水(PBS)中伤寒杆菌的检测限为 1 CFU/mL,线性检测范围为 100-107 CFU/mL。在实际样品中,该传感器在牛奶中的检测限为 5 CFU/mL,在鸡肉中的检测限为 3 CFU/mL,线性检测范围为 100 至 106 CFU/mL,保持了较高的准确性和可重复性。该生物传感器还能有效区分活的和死的细菌细胞,证明了其在实际食品安全应用中的潜力。该生物传感器在较宽的 pH 值范围(4-10)内都表现出色,并且在长达六周的时间内都保持稳定。总之,所开发的 BC/Ppy/RGO-phage 生物传感器为快速、灵敏、选择性地检测鼠伤寒杆菌提供了一种很有前途的工具,在不同的食品基质中都具有稳定的性能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ultrasensitive Electrochemical Detection of Salmonella typhimurium in Food Matrices Using Surface-Modified Bacterial Cellulose with Immobilized Phage Particles.

The rapid and sensitive detection of Salmonella typhimurium in food matrices is crucial for ensuring food safety. This study presents the development of an ultrasensitive electrochemical biosensor using surface-modified bacterial cellulose (BC) integrated with polypyrrole (Ppy) and reduced graphene oxide (RGO), further functionalized with immobilized S. typhimurium-specific phage particles. The BC substrate, with its ultra-fibrous and porous structure, was modified through in situ oxidative polymerization of Ppy and RGO, resulting in a highly conductive and flexible biointerface. The immobilization of phages onto this composite was facilitated by electrostatic interactions between the polycationic Ppy and the negatively charged phage capsid heads, optimizing phage orientation and enhancing bacterial capture efficiency. Morphological and chemical characterization confirmed the successful fabrication and phage immobilization. The biosensor demonstrated a detection limit of 1 CFU/mL for S. typhimurium in phosphate-buffered saline (PBS), with a linear detection range spanning 100 to 107 CFU/mL. In real samples, the sensor achieved detection limits of 5 CFU/mL in milk and 3 CFU/mL in chicken, with a linear detection range spanning 100 to 106 CFU/mL, maintaining high accuracy and reproducibility. The biosensor also effectively discriminated between live and dead bacterial cells, demonstrating its potential in real-world food safety applications. The biosensor performed excellently over a wide pH range (4-10) and remained stable for up to six weeks. Overall, the developed BC/Ppy/RGO-phage biosensor offers a promising tool for the rapid, sensitive, and selective detection of S. typhimurium, with robust performance across different food matrices.

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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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