利用小鼠模型诱导体内细胞标记并追踪标记的造血细胞。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-10-21 DOI:10.1016/j.xpro.2024.103408

Alina Syed, Michihiro Kobayashi, Momoko Yoshimoto

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引用次数: 0

摘要

结合造血干细胞（HSC）和内皮细胞（EC）血缘追踪小鼠模型，我们可以确定血细胞的起源。我们介绍了一种诱导体内细胞标记和追踪标记造血细胞以分离其起源的方案。我们描述了收获各种造血组织、抗体染色和分析每个免疫细胞群中 Tomato+ 百分比的步骤。我们还展示了如何估算目标细胞群中造血干细胞和造血干细胞来源的百分比。有关该方案使用和执行的完整细节，请参阅 Kobayashi 等人的文章1。

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Protocol to induce cell labeling in vivo and to trace labeled hematopoietic cells using mouse models.

A combination of hematopoietic stem cell (HSC)- and endothelial cell (EC)-lineage-tracing mouse models enables us to determine blood cell origins. We present a protocol to induce cell labeling in vivo and to trace labeled hematopoietic cells to segregate their origins. We describe the steps for harvesting various hematopoietic tissues, antibody staining, and analyzing the Tomato+ percentages within each immune cell population. We also show how to estimate HSC- and EC-derived percentages of the target cell populations. For complete details on the use and execution of this protocol, please refer to Kobayashi et al.¹.

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