Holothuria polii 提取物作为一种潜在的抗球虫剂:小鼠空肠中 MUC2 调节作用的证据

IF 2 2区 农林科学 Q2 VETERINARY SCIENCES
Youssef A El-Sayed, Ahmed E Abdel-Moneim, Rania G Taha, Mona F Khalil, Rewaida Abdel-Gaber, Felwa A Thagfan, Esam S Al-Malki, Mohamed A Dkhil
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引用次数: 0

摘要

艾美耳菌是一种寄生虫,会导致多种动物患上球虫病,造成宿主营养失衡、食物消化吸收障碍、体重过轻以及不同程度的肠道炎症。抗球虫药物的抗药性已成为疾病控制工作的一大障碍,因此人们重新开始关注发现新型化学物质以替代治疗方法。本研究旨在确定 Holothuria polii 提取物(HpE)治疗小鼠埃默里病的疗效。研究对象为五组小鼠,用乳头蚴孢子化卵囊(1 × 103)感染三组小鼠进行实验。第一组用 200 毫克/千克的 HpE 治疗,第二组用安普瑞林(120 毫克/千克)治疗,第三组不治疗作为对照。第四组和第五组未感染,其中一组接受 200 毫克/千克的 HpE 治疗,另一组作为阴性对照。GC-MS 分析结果显示,HpE 含有 10 种植物化学物质。经 HpE 处理后,粪便中的卵囊数量大幅减少。此外,寄生阶段的形态也发生了改变,与受感染的未处理小鼠相比,测量值有所下降。此外,与受感染的小鼠相比,经处理的小鼠体内粘蛋白基因(MUC2)的表达量更高,而腺泡细胞的数量也显著增加,这为 HpE 作为抗球虫产品的活性提供了更多证据。总之,天然产品的种类繁多,许多产品已被用于治疗各种寄生虫病的民间医药中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Holothuria polii Extract as a Potential Anticoccidial Agent: Evidence of Its MUC2 Regulatory Impact in Murine Jejunum.

Eimeria is a parasite that causes coccidiosis in a variety of animals, leading to nutritional imbalances, issues with food digestion and absorption, low weight, and intestinal inflammation of varying degrees in its hosts. Anticoccidial medication resistance has become a significant obstacle to disease control efforts, leading to a renewed focus on discovering novel chemicals for alternative therapeutic approaches. The purpose of this study was to determine the efficacy of Holothuria polii extract (HpE) in treating eimeriosis in mice. Five groups of mice were studied, with E. papillata sporulated oocysts (1 × 103) used to infect three groups experimentally. The first group was treated with 200 mg/kg of HpE, the second group with Amprolium (120 mg/kg), and the third group was left untreated as a control. The fourth and fifth groups were uninfected, with one of them treated with 200 mg/kg of HpE and the other used as a negative control. The results revealed that HpE has 10 phytochemical compounds according to GC-MS analysis. Treatment with HpE resulted in a substantial decrease in oocyst output in feces. Also, the parasitic stages showed morphometric alterations, with reductions in the measurements compared with the infected, untreated mice. Moreover, expression of the mucin gene (MUC2) was higher in treated mice compared to infected ones, with significant increases in goblet cell numbers, which provided additional evidence for the activity of HpE as an anticoccidial product. In conclusion, there are a wide variety of natural products and many have been employed in folk medicine for treating a range of parasitic diseases.

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来源期刊
Veterinary Sciences
Veterinary Sciences VETERINARY SCIENCES-
CiteScore
2.90
自引率
8.30%
发文量
612
审稿时长
6 weeks
期刊介绍: Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.
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