在中国江苏分离到的水痘-带状疱疹病毒的基因型。

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2024-10-24 DOI:10.1186/s12985-024-02543-3

Yong Wang, Lei Zhang, Mengqi Bian, Hongxiong Guo, Zhiguo Wang, Ying Hu, Xiuying Deng, Xiang Sun, Jun Ren

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引用次数: 0

摘要

目的分析江苏省水痘病毒的基因型，确定疫苗株和野生株，为有效预防和控制水痘提供分子生物学背景：方法：采用分层抽样的方法采集水痘患者的疱疹液或咽拭子。方法：采用分层抽样的方法，采集确诊为水痘患者的疱疹液或咽拭子，进行ORF22、ORF38、ORF54和ORF62的限制性酶切位点检测：结果：通过对 ORF22 的 PCR 产物进行测序，所有 207 株病毒均为支系 2 型。测序结果显示，与 Dumas 参考株（Clade 1）相比，有五个 SNP 位点发生了变化。从 A 变为 G 的位点为 37 902 个，从 T 变为 c 的位点为 38 055 个，从 A 变为 C 的位点为 38 081 个，从 G 变为 A 的位点为 38 177 个，从 G 变为 A 的位点为 39 394 个。江苏流行的 VZV 基因型与 P-Oka 型一致。对ORF38（PstI）、ORF54（BglI）和ORF62（SmaI）的PCR扩增产物进行限制酶切位点分析表明，207株病毒均为野生型。其中 183 株（88.4%）为 PstI（+）、BglI（+）、SmaI（-）型。不同地区之间的野生毒株无显著差异（χ2 = 0.05，P = 0.982）：结论：江苏省流行的 VZV 基因型为支系 2，流行的病毒株为野毒株，主要野毒株为 PstI（+）、BglI（+）、SmaI（-）。

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Genotype of Varicella-zoster virus isolated in Jiangsu, China.

Objective: To analyze the genotypes of VZV in Jiangsu province to identify vaccine strains and wild strains, providing a molecular biological background for the effective prevention and control of varicella.

Method: Stratified sampling was used to collect herpes fluid or throat swab from patients diagnosed with varicella. ORF22 was carried out, and the restriction enzyme site of ORF38, ORF54 and ORF62 were detected.

Results: All 207 virus strains were Clade 2 type by sequencing the PCR products of ORF22. The sequencing results showed that five SNP sites changed compared to the Dumas reference strain(Clade 1). From A to G at 37,902, from T to c at 38,055, from A to C at 38,081, and from G to A at 38,177, from G to A at 39,394. The prevalent VZV genotypes in Jiangsu is consistent with the P-Oka. The restriction enzyme site analysis of PCR amplification products from ORF38 (PstI), ORF54 (BglI), ORF62 (SmaI) showed that all 207 virus strains were wild-type. There were two different types of the wild strains, and 183 strains (88.4%) were PstI (+), BglI (+), SmaI (-). The wild strains between different regions showed no significant differences (χ² = 0.05, P = 0.982).

Conclusions: The prevalent VZV genotypes are Clade 2 and the prevalent virus strains are wild strains in Jiangsu Province, the primary wild strain observed is mainly PstI (+), BglI (+), SmaI (-).

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来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.