重组禽源性抗病毒蛋白 cIFITM1、cIFITM3 和 cViperin 作为 H9N2 亚型禽流感灭活疫苗的有效佐剂。

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2024-10-18 DOI:10.1016/j.vetmic.2024.110277

Shihong Yan , Yikai Chen , Jin Lin , Huimin Chen , Chenqi Hu , Hongyang Liu , Hongxiu Diao , Shasha Liu , Ji-Long Chen

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引用次数: 0

摘要

疫苗佐剂作为非特异性免疫增强剂，在动物疾病的免疫预防和控制中发挥着举足轻重的作用。本研究利用原核表达系统表达和纯化鸡源性 cIFITM1、cIFITM3 和 cViperin，然后将其作为佐剂与 H9N2 禽流感病毒抗原配制成灭活疫苗。将这些疫苗注射给 SPF 鸡，以研究它们的免疫增强功能。此外，还评估了这些蛋白质作为独立免疫增强剂的能力。结果表明，cIFITM1、cIFITM3 和 cViperin 能显著提高血清血凝抑制（HI）抗体滴度。值得注意的是，当单独使用这些蛋白时，它们能明显增强受挑战鸡的抗病毒能力，从而缓解临床症状、减少气管病毒复制、减少病毒脱落和减轻组织病理学损伤，其中 cIFITM1 的效果最为明显。此外，两种添加了 cIFITM1 佐剂的 H9N2 重组病毒灭活疫苗的保护效力也得到了验证，疫苗保护效率达到了 100%。总之，cIFITM1、cIFITM3 和 cViperin 作为流感疫苗佐剂可有效抑制病毒复制和脱落，在流感防控中具有重要潜力。

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Recombinant avian-derived antiviral proteins cIFITM1, cIFITM3, and cViperin as effective adjuvants in inactivated H9N2 subtype avian influenza vaccines

Vaccine adjuvants, serving as non-specific immune enhancers, play a pivotal role in the immunoprevention and control of animal diseases. This study utilized prokaryotic expression systems to express and purify chicken-derived cIFITM1, cIFITM3, and cViperin, which were then formulated as adjuvants with H9N2 avian influenza virus antigens to create inactivated vaccines. These vaccines were administered to SPF chickens to investigate their immunopotentiating functions. Additionally, the proteins were assessed for their ability to act as standalone immune enhancers. The results demonstrated that cIFITM1, cIFITM3, and cViperin significantly elevated serum hemagglutination inhibition (HI) antibody titers. Notably, when used individually, these proteins markedly enhanced the antiviral capabilities of challenged chickens, leading to alleviated clinical symptoms, reduced tracheal virus replication, diminished virus shedding, and lessened histopathological damage, with cIFITM1 exhibiting the most pronounced effect. Furthermore, the protective efficacy of two H9N2 recombinant virus inactivated vaccines supplemented with cIFITM1 adjuvant was validated, achieving a 100 % vaccine protection efficiency. In conclusion, cIFITM1, cIFITM3, and cViperin as adjuvants for influenza vaccines effectively inhibit virus replication and shedding, highlighting their significant potential in influenza prevention and control.

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.