Mikela Vlachou, Andreana Pexara, Nikolaos Solomakos, Alexander Govaris, Dimitrios Palaiogiannis, Vassilis Athanasiadis, Stavros I Lalas
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引用次数: 0
摘要
这项研究旨在评估希腊屠宰猪血清中的赭曲霉毒素A（OTA）残留量。样本来自希腊8个不同地区21个区域单位的113个不同农场的1695头健康屠宰猪，并采用免疫吸附分析法（ELISA）和高效液相色谱-荧光检测器（HPLC-FD）进行分析。OTA 污染评估显示，分别有 782 份（46.1%）和 1233 份（72.7%）样本 OTA 阳性，根据 ELISA 和 HPLC-FD 分析，浓度范围分别为 0.20-5.38 微克/升和 0.15-5.96 微克/升。此外，通过 ELISA 和 HPLC-FD 分析，分别发现 88 个（77.9%）和 108 个（95.6%）猪场的 OTA 呈阳性。Thessaly 地区的 OTA 血清阳性率（>98%）和 HPLC-FD 测定的毒素水平（5.96 µg/L）最高，而克里特岛地区猪场的 OTA 阳性率高达 100%（范围 75-100%）。在希腊不同地区的屠宰猪血清中检测到 OTA 会对动物和人类健康造成威胁，因此需要对养猪业和猪肉生产设施进行持续的 OTA 监测。
Presence of Ochratoxin A Residues in Blood Serum of Slaughtered Pigs in Greece.
This study aimed to assess the presence of ochratoxin A (OTA) residues in the blood serum of slaughtered pigs in Greece. Samples were obtained from 1695 healthy slaughtered pigs originating from 113 different farms located in 21 geographic regional units in 8 different geographic regions of Greece and were analyzed using an immunosorbent assay (ELISA) and high-performance liquid chromatography with fluorescence detector (HPLC-FD). OTA contamination assessment showed that 782 (46.1%) and 1233 (72.7%) samples were OTA-positive, with a concentration range of 0.20-5.38 μg/L and 0.15-5.96 µg/L according to ELISA and HPLC-FD analysis, respectively. Also, 88 (77.9%) and 108 (95.6%) of farms were found to be OTA-positive by ELISA and HPLC-FD analysis, respectively. The highest OTA serum positivity rate (>98%) and toxin level (5.96 µg/L) determined by HPLC-FD were observed in the Thessaly region, whereas a high prevalence of up to 100% (range 75-100%) was found on farms in the Crete Island region. The detection of OTA in the serum of slaughtered pigs in different regions in Greece poses a risk for animal and human health and highlights the need for constant OTA monitoring in the swine industry and pork meat production facilities.
期刊介绍：
Toxins (ISSN 2072-6651) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to toxins and toxinology. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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