Nadia Sawicka-Gutaj, Piotr Glinicki, Kacper Nijakowski, Barbara Bromińska, Magdalena Ostrowska, Alicja Szatko, Zuzanna Sobol, Konrad Kowalski, Paweł Wilk, Wojciech Zgliczyński, Marek Ruchała
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We measured TSH (Thyroid-Stimulating Hormone), free T3 (fT3), and free T4 (fT4) in the blood using two different immunoassay methods: electrochemiluminescence immunoassay (ECLIA) and chemiluminescence immunoassay (CLIA). Saliva samples were collected using Salivette<sup>®</sup> tubes, and then we analyzed the levels of thyroid hormones in the saliva using liquid chromatography with tandem mass spectrometry (LC-MS/MS).</p><p><strong>Results: </strong>Comparing salivary and serum levels of thyroid hormones, we observed significantly lower fT4 and higher fT3 levels in saliva (<i>p</i> < 0.001), regardless of the measurement method. We also found that salivary fT3 and fT4 levels were positively correlated in the entire group of participants (<i>p</i> < 0.001, <i>R</i> 0.575), as well as in those with TSH values within the normal reference range (<i>p</i> < 0.001, <i>R</i> 0.570). We further divided participants based on their TSH reference intervals and assessed the correlations between serum and salivary thyroid hormones within these groups. We also discovered that taking exogenous levothyroxine influenced the correlations between serum and salivary thyroid hormones. Thyroid antibodies did not have a significant impact on the levels of thyroid hormones in both serum and saliva.</p><p><strong>Conclusion: </strong>This study demonstrates that salivary thyroid hormones can partially reflect the levels of these hormones in the blood. 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引用次数: 0
摘要
背景：唾液可以替代血液评估甲状腺激素功能：唾液可以替代血液评估甲状腺激素功能：我们的目标是确定唾液中的甲状腺激素水平能否准确反映一个人的甲状腺状态，以及唾液中的甲状腺激素水平是否与血液中的相同激素水平一致：设计：我们进行了一项横断面研究：方法：我们共招募了 109 名患有不同甲状腺疾病的参与者。我们使用两种不同的免疫测定方法测量了血液中的促甲状腺激素（TSH）、游离 T3（fT3）和游离 T4（fT4）：电化学发光免疫测定（ECLIA）和化学发光免疫测定（CLIA）。我们使用 Salivette® 管采集唾液样本，然后使用液相色谱-串联质谱法（LC-MS/MS）分析唾液中的甲状腺激素水平：比较唾液和血清中的甲状腺激素水平，我们发现唾液中的 fT4 水平明显较低，fT3 水平明显较高（p p R 0.575），TSH 值在正常参考范围内的人唾液中的 fT4 水平也明显较低（p R 0.570）。我们根据参与者的促甲状腺激素参考区间对他们进行了进一步划分，并评估了这些组别中血清和唾液甲状腺激素之间的相关性。我们还发现，服用外源性左甲状腺素会影响血清和唾液甲状腺激素之间的相关性。甲状腺抗体对血清和唾液中的甲状腺激素水平没有显著影响：这项研究表明，唾液中的甲状腺激素可以部分反映血液中的甲状腺激素水平。在临床环境中，唾液可能是甲状腺抗体患者的一个有用工具，但对于正在服用左甲状腺素治疗的患者来说，唾液可能并不那么有效。
Measurement of salivary thyroid hormones using the LC-MS/MS technique in a clinical setting.
Background: Saliva could be an attractive alternative to blood for assessing thyroid hormonal function.
Objectives: Our goal was to determine if the levels of thyroid hormones in saliva can accurately reflect a person's thyroid status and if they consistently correlate with the levels of the same hormones in the blood.
Design: We conducted a cross-sectional study.
Methods: We enrolled a total of 109 participants with different thyroid conditions. We measured TSH (Thyroid-Stimulating Hormone), free T3 (fT3), and free T4 (fT4) in the blood using two different immunoassay methods: electrochemiluminescence immunoassay (ECLIA) and chemiluminescence immunoassay (CLIA). Saliva samples were collected using Salivette® tubes, and then we analyzed the levels of thyroid hormones in the saliva using liquid chromatography with tandem mass spectrometry (LC-MS/MS).
Results: Comparing salivary and serum levels of thyroid hormones, we observed significantly lower fT4 and higher fT3 levels in saliva (p < 0.001), regardless of the measurement method. We also found that salivary fT3 and fT4 levels were positively correlated in the entire group of participants (p < 0.001, R 0.575), as well as in those with TSH values within the normal reference range (p < 0.001, R 0.570). We further divided participants based on their TSH reference intervals and assessed the correlations between serum and salivary thyroid hormones within these groups. We also discovered that taking exogenous levothyroxine influenced the correlations between serum and salivary thyroid hormones. Thyroid antibodies did not have a significant impact on the levels of thyroid hormones in both serum and saliva.
Conclusion: This study demonstrates that salivary thyroid hormones can partially reflect the levels of these hormones in the blood. Saliva may be a useful tool in a clinical setting for patients with thyroid antibodies, but it may not be as effective for those who are taking levothyroxine as a treatment.
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Therapeutic Advances in Endocrinology and Metabolism delivers the highest quality peer-reviewed articles, reviews, and scholarly comment on pioneering efforts and innovative studies across all areas of endocrinology and metabolism.
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