从一名老年女性身上建立诱导多能细胞系（ABCRIi001-A），用于老龄化研究。

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2024-10-15 DOI:10.1016/j.scr.2024.103576

Filipa Esteves , David Brito , Ana Teresa Rajado , Nádia Silva , Joana Apolónio , Vânia Palma Roberto , ALFA Score Consortium, Raquel P. Andrade , Sofia Calado , Maria Leonor Faleiro , Carlos Matos , Nuno Marques , Ana Marreiros , Hipólito Nzwalo , Sandra Pais , Isabel Palmeirim , Sónia Simãoa , Natércia Joaquim , Rui Miranda , António Pêgas , José Bragança

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引用次数: 0

摘要

人类诱导多能干细胞（hiPSC）有望模拟和了解人类疾病，包括与衰老相关的疾病。在这里，我们描述了ABCRIi001-A，这是一种从一名79岁女性的外周血单核细胞（PBMC）中产生的hiPSC细胞系，该女性参加了一项老龄化评分（ALFA评分）的研究。使用三种基于仙台病毒的重编程载体（hKOS、hc-Myc 和 hKlf4）对 PBMC 进行了重编程。ABCRIi001-A显示出正常的形态和核型、病毒清除、无基因组畸变，其多能相关标记物的表达和向三个生殖层分化的能力证实了它们的多能性。ABCRIi001-A对老龄化相关研究具有重要价值。

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Establishment of an induced pluripotent cell line (ABCRIi001-A) from an elderly female for ageing research

Human induced pluripotent stem cells (hiPSCs) hold promises to model and understand human diseases, including those associated with ageing. Here, we describe ABCRIi001-A, a hiPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female enrolled in a study for development of an ageing score (ALFA Score). PBMCs were reprogrammed using three Sendai virus-based reprogramming vectors (hKOS, hc-Myc, and hKlf4). ABCRIi001-A showed normal morphology and karyotype, viral clearance, absence of genomic aberrations, and their pluripotency was confirmed by expression of pluripotency-related markers and their ability to differentiate into the three germ layers. ABCRIi001-A is valuable for ageing-related studies.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.