与幼年期有关的对木瓜粘连病(PSD)的耐受性:蛋白质组、超微结构和生理事件。

IF 5.3 2区 生物学 Q1 PLANT SCIENCES
Silas P Rodrigues, Eduardo de A Soares, Tathiana F Sá Antunes, Marlonni Maurastoni, Leidy J Madroñero, Sabrina G Broetto, Lucas E C Nunes, Brunno R F Verçoza, David S Buss, Diolina M Silva, Juliany C F Rodrigues, José A Ventura, Patricia M B Fernandes
{"title":"与幼年期有关的对木瓜粘连病(PSD)的耐受性:蛋白质组、超微结构和生理事件。","authors":"Silas P Rodrigues, Eduardo de A Soares, Tathiana F Sá Antunes, Marlonni Maurastoni, Leidy J Madroñero, Sabrina G Broetto, Lucas E C Nunes, Brunno R F Verçoza, David S Buss, Diolina M Silva, Juliany C F Rodrigues, José A Ventura, Patricia M B Fernandes","doi":"10.1007/s00299-024-03358-w","DOIUrl":null,"url":null,"abstract":"<p><strong>Key message: </strong>The proteomic analysis of PMeV-complex-infected C. papaya unveiled proteins undergoing modulation during the plant's development. The infection notably impacted processes related to photosynthesis and cell wall dynamics. The development of Papaya Sticky Disease (PSD), caused by the papaya meleira virus complex (PMeV-complex), occurs only after the juvenile/adult transition of Carica papaya plants, indicating the presence of tolerance mechanisms during the juvenile development phase. In this study, we quantified 1609 leaf proteins of C. papaya using a label-free strategy. A total of 345 differentially accumulated proteins were identified-38 at 3 months (juvenile), 130 at 4 months (juvenile/adult transition), 160 at 7 months (fruit development), and 17 at 9 months (fruit harvesting)-indicating modulation of biological processes at each developmental phase, primarily related to photosynthesis and cell wall remodeling. Infected 3- and 4-mpg C. papaya exhibited an accumulation of photosynthetic proteins, and chlorophyll fluorescence results suggested enhanced energy flux efficiency in photosystems II and I in these plants. Additionally, 3 and 4-mpg plants showed a reduction in cell wall-degrading enzymes, followed by an accumulation of proteins involved in the synthesis of wall precursors during the 7 and 9-mpg phases. These findings, along with ultrastructural data on laticifers, indicate that C. papaya struggles to maintain the integrity of laticifer walls, ultimately failing to do so after the 4-mpg phase, leading to latex exudation. This supports initiatives for the genetic improvement of C. papaya to enhance resistance against the PMeV-complex.</p>","PeriodicalId":20204,"journal":{"name":"Plant Cell Reports","volume":null,"pages":null},"PeriodicalIF":5.3000,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Juvenile-related tolerance to papaya sticky disease (PSD): proteomic, ultrastructural, and physiological events.\",\"authors\":\"Silas P Rodrigues, Eduardo de A Soares, Tathiana F Sá Antunes, Marlonni Maurastoni, Leidy J Madroñero, Sabrina G Broetto, Lucas E C Nunes, Brunno R F Verçoza, David S Buss, Diolina M Silva, Juliany C F Rodrigues, José A Ventura, Patricia M B Fernandes\",\"doi\":\"10.1007/s00299-024-03358-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Key message: </strong>The proteomic analysis of PMeV-complex-infected C. papaya unveiled proteins undergoing modulation during the plant's development. The infection notably impacted processes related to photosynthesis and cell wall dynamics. The development of Papaya Sticky Disease (PSD), caused by the papaya meleira virus complex (PMeV-complex), occurs only after the juvenile/adult transition of Carica papaya plants, indicating the presence of tolerance mechanisms during the juvenile development phase. In this study, we quantified 1609 leaf proteins of C. papaya using a label-free strategy. A total of 345 differentially accumulated proteins were identified-38 at 3 months (juvenile), 130 at 4 months (juvenile/adult transition), 160 at 7 months (fruit development), and 17 at 9 months (fruit harvesting)-indicating modulation of biological processes at each developmental phase, primarily related to photosynthesis and cell wall remodeling. Infected 3- and 4-mpg C. papaya exhibited an accumulation of photosynthetic proteins, and chlorophyll fluorescence results suggested enhanced energy flux efficiency in photosystems II and I in these plants. Additionally, 3 and 4-mpg plants showed a reduction in cell wall-degrading enzymes, followed by an accumulation of proteins involved in the synthesis of wall precursors during the 7 and 9-mpg phases. These findings, along with ultrastructural data on laticifers, indicate that C. papaya struggles to maintain the integrity of laticifer walls, ultimately failing to do so after the 4-mpg phase, leading to latex exudation. This supports initiatives for the genetic improvement of C. papaya to enhance resistance against the PMeV-complex.</p>\",\"PeriodicalId\":20204,\"journal\":{\"name\":\"Plant Cell Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2024-10-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Cell Reports\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00299-024-03358-w\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Cell Reports","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00299-024-03358-w","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

关键信息:对受 PMeV 复合物感染的番木瓜进行的蛋白质组分析揭示了在植物发育过程中发生调控的蛋白质。感染明显影响了光合作用和细胞壁动力学的相关过程。由木瓜髓病毒复合体(PMeV-complex)引起的木瓜粘连病(PSD)只发生在木瓜植株的幼年/成年过渡期之后,这表明在幼年发育阶段存在耐受机制。在这项研究中,我们采用无标记策略对木瓜的 1609 种叶片蛋白质进行了量化。共鉴定出 345 种不同积累的蛋白质,其中 3 个月(幼苗期)38 种,4 个月(幼苗/成株过渡期)130 种,7 个月(果实发育期)160 种,9 个月(果实采收期)17 种,这表明每个发育阶段的生物过程都受到了调节,主要与光合作用和细胞壁重塑有关。受感染的 3 个月和 4 个月木瓜表现出光合蛋白的积累,叶绿素荧光结果表明这些植株光合系统 II 和 I 的能量通量效率提高。此外,3-mpg 和 4-mpg 期的木瓜植株细胞壁降解酶减少,7-mpg 和 9-mpg 期的木瓜植株细胞壁前体合成蛋白积累。这些发现以及关于叶片的超微结构数据表明,木瓜努力维持叶片壁的完整性,最终在 4 个叶片期后失败,导致乳汁渗出。这为木瓜的遗传改良提供了支持,以增强对 PMeV 复合体的抗性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Juvenile-related tolerance to papaya sticky disease (PSD): proteomic, ultrastructural, and physiological events.

Key message: The proteomic analysis of PMeV-complex-infected C. papaya unveiled proteins undergoing modulation during the plant's development. The infection notably impacted processes related to photosynthesis and cell wall dynamics. The development of Papaya Sticky Disease (PSD), caused by the papaya meleira virus complex (PMeV-complex), occurs only after the juvenile/adult transition of Carica papaya plants, indicating the presence of tolerance mechanisms during the juvenile development phase. In this study, we quantified 1609 leaf proteins of C. papaya using a label-free strategy. A total of 345 differentially accumulated proteins were identified-38 at 3 months (juvenile), 130 at 4 months (juvenile/adult transition), 160 at 7 months (fruit development), and 17 at 9 months (fruit harvesting)-indicating modulation of biological processes at each developmental phase, primarily related to photosynthesis and cell wall remodeling. Infected 3- and 4-mpg C. papaya exhibited an accumulation of photosynthetic proteins, and chlorophyll fluorescence results suggested enhanced energy flux efficiency in photosystems II and I in these plants. Additionally, 3 and 4-mpg plants showed a reduction in cell wall-degrading enzymes, followed by an accumulation of proteins involved in the synthesis of wall precursors during the 7 and 9-mpg phases. These findings, along with ultrastructural data on laticifers, indicate that C. papaya struggles to maintain the integrity of laticifer walls, ultimately failing to do so after the 4-mpg phase, leading to latex exudation. This supports initiatives for the genetic improvement of C. papaya to enhance resistance against the PMeV-complex.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信