壳斗属植物染色体级基因组组装和功能注释:为抗旱作物开发挖掘基因资源。

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-10-23 DOI:10.1007/s00425-024-04551-7
Anestis Gkanogiannis, Hifzur Rahman, Rakesh Kumar Singh, Augusto Becerra Lopez-Lavalle
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引用次数: 0

摘要

主要结论染色体水平的西瓜基因组组装揭示了其提高耐旱性的遗传潜力,为创新作物改良战略铺平了道路。本研究首次对西瓜(Citrullus colocynthis)进行了全面的基因组组装和注释,西瓜是栽培西瓜的一种耐旱野生近缘植物,本研究强调了西瓜在提高农业对气候变化的适应能力方面的潜力。这项研究利用先进的测序技术(包括 PacBio HiFi 和 Hi-C)实现了染色体组水平的组装,发现基因组大小约为 366 Mb,具有低杂合度和大量重复内容。我们的分析确定了 23,327 个基因模型,它们可能编码物种适应干旱环境的应激反应机制。与近缘物种的比较基因组学揭示了葫芦科内的进化动态。此外,对来自阿拉伯联合酋长国(UAE)的 27 个品种进行的重新测序发现了遗传多样性,为未来的育种计划奠定了基础。这一基因组资源为从头驯化 C. colocynthis 开辟了新途径,为开发具有更强耐旱性、抗病性和营养特征的作物提供了蓝图,这对于在气候挑战不断升级的情况下维持未来的粮食安全至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chromosome-level genome assembly and functional annotation of Citrullus colocynthis: unlocking genetic resources for drought-resilient crop development.

Main conclusion: The chromosome-level genome assembly of Citrullus colocynthis reveals its genetic potential for enhancing drought tolerance, paving the way for innovative crop improvement strategies. This study presents the first comprehensive genome assembly and annotation of Citrullus colocynthis, a drought-tolerant wild close relative of cultivated watermelon, highlighting its potential for enhancing agricultural resilience to climate change. The study achieved a chromosome-level assembly using advanced sequencing technologies, including PacBio HiFi and Hi-C, revealing a genome size of approximately 366 Mb with low heterozygosity and substantial repetitive content. Our analysis identified 23,327 gene models, that could encode stress response mechanisms for species' adaptation to arid environments. Comparative genomics with closely related species illuminated the evolutionary dynamics within the Cucurbitaceae family. In addition, resequencing of 27 accessions from the United Arab Emirates (UAE) identified genetic diversity, suggesting a foundation for future breeding programs. This genomic resource opens new avenues for the de novo domestication of C. colocynthis, offering a blueprint for developing crops with enhanced drought tolerance, disease resistance, and nutritional profiles, crucial for sustaining future food security in the face of escalating climate challenges.

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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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