弓形虫 WH3 Δrop18 可作为预防急性和慢性弓形虫病的减毒活疫苗。

IF 6.9 1区医学 Q1 IMMUNOLOGY

NPJ Vaccines Pub Date : 2024-10-23 DOI:10.1038/s41541-024-00996-9

Cong Wang, Shengnan Fu, Xin Yu, Hang Zhou, Famin Zhang, Lingling Song, Ji Zhao, Yun Yang, Jianbing Du, Qingli Luo, Jilong Shen, Li Yu

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引用次数: 0

摘要

弓形虫是人畜共患弓形虫病的重要病原体。本研究开发了一种 WH3 Δrop18 弓形虫减毒活疫苗。结果表明，所有接种了 WH3 Δrop18 疫苗的小鼠都能在各种弓形虫菌株（包括 RH（I 型）、ME49（II 型）、WH3 或 WH6（中国 1 型））的挑战下存活。接种过 ME49 或 WH6 疫苗的动物在受到形成囊肿的菌株挑战后，脑部未发现囊肿，即使有也为数不多。接种WH3 Δrop18疫苗后，动物产生了强烈的免疫反应，包括细胞因子（IFN-γ、IL-12、TNF-α和IL-10）水平显著升高、CD4+和CD8+T淋巴细胞活化以及针对弓形虫的特异性抗体长期存在。我们的研究结果有力地表明，WH3 Δrop18 疫苗可针对多种弓形虫感染株提供有效的免疫保护，是一种很有前途的减毒活疫苗候选物。

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Toxoplasma WH3 Δrop18 acts as a live attenuated vaccine against acute and chronic toxoplasmosis.

Toxoplasma gondii is a significant zoonotic pathogen of toxoplasmosis in humans and animals. Here a live attenuated Toxoplasma vaccine of WH3 Δrop18 was developed. The results showed that all mice vaccinated with WH3 Δrop18 were able to survive when challenge with various strains of Toxoplasma, including RH (type I), ME49 (type II), WH3 or WH6 (type Chinese 1). No cysts, if few, in the brain of the vaccinated animals were seen after challenge with cyst forming strains of ME49 or WH6. Vaccination with the WH3 Δrop18 triggered a strong immune response, including significantly increased level of the cytokines (IFN-γ, IL-12, TNF-α and IL-10) and the activation of CD4⁺ and CD8⁺ T-lymphocytes and long term of specific antibodies against Toxoplasma. Our results strongly indicate that vaccine of WH3 Δrop18 might provide effective immune protection against a wide range strains of Toxoplasma infections and be a promising live attenuated vaccine candidate.

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来源期刊

NPJ Vaccines Immunology and Microbiology-Immunology

CiteScore

11.90

自引率

4.30%

发文量

146

审稿时长

11 weeks

期刊介绍： Online-only and open access, npj Vaccines is dedicated to highlighting the most important scientific advances in vaccine research and development.