利用脱细胞生物支架生成猪人工肠道

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS
Sharon Arcuri, Georgia Pennarossa, Madhusha Prasadani, Fulvio Gandolfi, Tiziana A L Brevini
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引用次数: 0

摘要

近年来,人们对能够模拟原生组织的生理结构和功能的高重现性三维体外模型的开发兴趣浓厚。迄今为止,已经提出了多种体外再造肠屏障的技术,包括合成支架和水凝胶,以及复杂的芯片系统和有机体。在这里,我们描述了一种基于脱细胞生物支架及其与肠道基质细胞和上皮细胞的再填充生成人工肠道的新方案。从当地屠宰场采集的器官要经过脱细胞处理,包括冷冻/解冻步骤,然后依次在1% SDS、1% Triton X-100和2%脱氧胆酸盐中孵育12小时。本文所描述的方案是一种很有前景的新策略,可用于生成体外生物工程肠道平台,该平台可模仿肠道屏障的某些复杂功能,从而为营养学、药理学和毒理学研究提供一种很有前景的三维策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Use of Decellularized Bio-Scaffolds for the Generation of a Porcine Artificial Intestine.

In recent years, great interest has been focused on the development of highly reproducible 3D in vitro models that are able to mimic the physiological architecture and functionality of native tissues. To date, a wide range of techniques have been proposed to recreate an intestinal barrier in vitro, including synthetic scaffolds and hydrogels, as well as complex on-a-chip systems and organoids. Here, we describe a novel protocol for the generation of an artificial intestine based on the creation of decellularized bio-scaffolds and their repopulation with intestinal stromal and epithelial cells. Organs collected at the local slaughterhouse are subjected to a decellularization protocol that includes a freezing/thawing step, followed by sequential incubation in 1% SDS for 12 h, 1% Triton X-100 for 12 h, and 2% deoxycholate for 12 h. At the end of the procedure, the generated bio-scaffolds are repopulated with intestinal fibroblasts and then with epithelial cells. The protocol described here represents a promising and novel strategy to generate an in vitro bioengineered intestine platform able to mimic some of the complex functions of the intestinal barrier, thus constituting a promising 3D strategy for nutritional, pharmaceutical, and toxicological studies.

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来源期刊
Methods and Protocols
Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
85
审稿时长
8 weeks
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