补充三氯蔗糖对超重或肥胖受试者血糖反应、食欲和肠道微生物群的影响:随机交叉研究方案。

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS
Zeniff Reyes-López, Viridiana Olvera-Hernández, Meztli Ramos-García, José D Méndez, Crystell G Guzmán-Priego, Miriam C Martínez-López, Carlos García-Vázquez, Carina S Alvarez-Villagomez, Isela E Juárez-Rojop, Juan C Díaz-Zagoya, Jorge L Ble-Castillo
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引用次数: 0

摘要

三氯蔗糖是最常见的非营养性甜味剂;然而,多年来它对新陈代谢的影响引发了巨大争议。我们旨在研究每天摄入三氯蔗糖对超重或肥胖受试者血糖、主观食欲和肠道微生物群(GM)变化的影响。在这项随机、交叉和对照试验中,23 名体重指数介于 25 kg/m2 和 39.9 kg/m2 之间的受试者将被分配到两种干预措施中的一种,接受三氯蔗糖(2 毫克/千克/天,相当于可接受日摄入量的 40%)或葡萄糖(对照组)干预,为期 4 周,每个阶段之间有 4 周的冲洗期。血糖反应将在耐受餐试验中确定,主观食欲将使用视觉模拟量表进行评估,转基因变化将通过粪便样本中细菌 rRNA 16S 基因的新一代测序进行分析。所有测量都将在干预期前后进行。我们假设,补充三氯蔗糖会诱导超重和肥胖参与者的血糖反应、主观食欲和肠道微生物群发生变化。本研究方案已获得澳大利亚犹太教大学伦理委员会批准(编号:0721),并在澳大利亚新西兰临床试验注册中心注册(ACTRN12621001531808)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Sucralose Supplementation on Glycemic Response, Appetite, and Gut Microbiota in Subjects with Overweight or Obesity: A Randomized Crossover Study Protocol.

Sucralose stands as the most common non-nutritive sweetener; however, its metabolic effects have sparked significant controversy over the years. We aim to examine the effects of sucralose daily intake on glycemia, subjective appetite, and gut microbiota (GM) changes in subjects with overweight or obesity. In this randomized, crossover, and controlled trial, 23 participants with a body mass index between 25 kg/m2 and 39.9 kg/m2 will be assigned to one of two interventions to receive either sucralose (2 mg/kg/day equivalent to 40% of the acceptable daily intake) or glucose (control) for 4 weeks, each phase separated by a 4-week washout period. The glycemic response will be determined during a meal tolerance test, subjective appetite will be evaluated using a visual analog scale, and GM changes will be analyzed by next-generation sequencing of the bacterial rRNA 16S gene from fecal samples. All measures will be performed before and after intervention periods. We hypothesize that sucralose supplementation induces changes in glycemic response, subjective appetite, and gut microbiota in overweight and obese participants. This protocol was approved by the Ethics Committee of the UJAT (No. 0721) and was registered in the Australian New Zealand Clinical Trials Registry (ACTRN12621001531808).

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来源期刊
Methods and Protocols
Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
85
审稿时长
8 weeks
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