多功能超高效液相色谱-全扫描高分辨率质谱法定量葡萄酒多酚。

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS

Methods and Protocols Pub Date : 2024-10-10 DOI:10.3390/mps7050082

Damien Flores, Emmanuelle Meudec, Aécio Luís de Sousa Dias, Nicolas Sommerer

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引用次数: 0

摘要

多酚是造成葡萄酒色泽和涩味的原因，作为抗氧化剂，它们还具有有益健康的特性。在这项工作中，我们使用 UHPLC-OrbitrapTM 系统开发了一种稳健的全扫描高分辨率质谱方法，用于定量检测葡萄酒样品（红葡萄酒、桃红葡萄酒或白葡萄酒）中的 90 种酚类化合物。利用这种方法，我们可以对红葡萄酒、桃红葡萄酒和白葡萄酒中的酚类化合物进行详细分析，其质量精确度达到了亚ppm级，具有很高的选择性。此外，通过全扫描光谱，我们还能监测样品中检测到的所有其他化合物，从而在需要时将该方法应用于新的酚类化合物。

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A Versatile Ultra-High-Performance Liquid Chromatography-Full-Scan High-Resolution Mass Spectrometry Method to Quantify Wine Polyphenols.

Polyphenols are responsible for wine colour and astringency, and, as antioxidants, they also have beneficial health properties. In this work, we developed a robust full-scan high-resolution mass spectrometry method for the quantification of 90 phenolic compounds in wine samples (either red, rosé, or white wine), using a UHPLC-Orbitrap^TM system. With this method, we could conduct a detailed analysis of phenolic compounds in red, rosé, and white wines with great selectivity due to sub-ppm mass accuracy. Moreover, accessing the full-scan spectrum enabled us to monitor all the other compounds detected in the sample, facilitating the adaptability of this method to new phenolic compounds if needed.

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来源期刊

Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

8 weeks