Cesar Arriagada-Escamilla, Roxana Alvarado, Javier Ortiz, Reinaldo Campos-Vargas, Pablo Cornejo
{"title":"藻酸盐-膨润土包裹极端嗜水细菌群能增强藜麦对金属应力的耐受性","authors":"Cesar Arriagada-Escamilla, Roxana Alvarado, Javier Ortiz, Reinaldo Campos-Vargas, Pablo Cornejo","doi":"10.3390/microorganisms12102066","DOIUrl":null,"url":null,"abstract":"<p><p>This study explores the encapsulation in alginate/bentonite beads of two metal(loid)-resistant bacterial consortia (consortium A: <i>Pseudomonas</i> sp. and <i>Bacillus</i> sp.; consortium B: <i>Pseudomonas</i> sp. and <i>Bacillus</i> sp.) from the Atacama Desert (northern Chile) and Antarctica, and their influence on physiological traits of <i>Chenopodium quinoa</i> growing in metal(loid)-contaminated soils. The metal(loid) sorption capacity of the consortia was determined. Bacteria were encapsulated using ionic gelation and were inoculated in soil of <i>C. quinoa</i>. The morphological variables, photosynthetic pigments, and lipid peroxidation in plants were evaluated. Consortium A showed a significantly higher biosorption capacity than consortium B, especially for As and Cu. The highest viability of consortia was achieved with matrices A1 (3% alginate and 2% bentonite) and A3 (3% alginate, 2% bentonite and 2.5% LB medium) at a drying temperature of 25 °C and storage at 4 °C. After 12 months, the highest viability was detected using matrix A1 with a concentration of 10<sup>6</sup> CFU g<sup>-1</sup>. Further, a greenhouse experiment using these consortia in <i>C. quinoa</i> plants showed that, 90 days after inoculation, the morphological traits of both consortia improved. Chemical analysis of metal(loid) contents in the leaves indicated that consortium B reduced the absorption of Cu to 32.1 mg kg<sup>-1</sup> and that of Mn to 171.9 mg kg<sup>-1</sup>. Encapsulation resulted in a significant increase in bacterial survival. This highlights the benefits of using encapsulated microbial consortia from extreme environments, stimulating the growth of <i>C. quinoa</i>, especially in soils with metal(loid) levels that can be a serious constraint for plant growth.</p>","PeriodicalId":18667,"journal":{"name":"Microorganisms","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509983/pdf/","citationCount":"0","resultStr":"{\"title\":\"Alginate-Bentonite Encapsulation of Extremophillic Bacterial Consortia Enhances <i>Chenopodium quinoa</i> Tolerance to Metal Stress.\",\"authors\":\"Cesar Arriagada-Escamilla, Roxana Alvarado, Javier Ortiz, Reinaldo Campos-Vargas, Pablo Cornejo\",\"doi\":\"10.3390/microorganisms12102066\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study explores the encapsulation in alginate/bentonite beads of two metal(loid)-resistant bacterial consortia (consortium A: <i>Pseudomonas</i> sp. and <i>Bacillus</i> sp.; consortium B: <i>Pseudomonas</i> sp. and <i>Bacillus</i> sp.) from the Atacama Desert (northern Chile) and Antarctica, and their influence on physiological traits of <i>Chenopodium quinoa</i> growing in metal(loid)-contaminated soils. The metal(loid) sorption capacity of the consortia was determined. Bacteria were encapsulated using ionic gelation and were inoculated in soil of <i>C. quinoa</i>. The morphological variables, photosynthetic pigments, and lipid peroxidation in plants were evaluated. Consortium A showed a significantly higher biosorption capacity than consortium B, especially for As and Cu. The highest viability of consortia was achieved with matrices A1 (3% alginate and 2% bentonite) and A3 (3% alginate, 2% bentonite and 2.5% LB medium) at a drying temperature of 25 °C and storage at 4 °C. After 12 months, the highest viability was detected using matrix A1 with a concentration of 10<sup>6</sup> CFU g<sup>-1</sup>. Further, a greenhouse experiment using these consortia in <i>C. quinoa</i> plants showed that, 90 days after inoculation, the morphological traits of both consortia improved. Chemical analysis of metal(loid) contents in the leaves indicated that consortium B reduced the absorption of Cu to 32.1 mg kg<sup>-1</sup> and that of Mn to 171.9 mg kg<sup>-1</sup>. Encapsulation resulted in a significant increase in bacterial survival. This highlights the benefits of using encapsulated microbial consortia from extreme environments, stimulating the growth of <i>C. quinoa</i>, especially in soils with metal(loid) levels that can be a serious constraint for plant growth.</p>\",\"PeriodicalId\":18667,\"journal\":{\"name\":\"Microorganisms\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-10-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11509983/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microorganisms\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3390/microorganisms12102066\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microorganisms","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/microorganisms12102066","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Alginate-Bentonite Encapsulation of Extremophillic Bacterial Consortia Enhances Chenopodium quinoa Tolerance to Metal Stress.
This study explores the encapsulation in alginate/bentonite beads of two metal(loid)-resistant bacterial consortia (consortium A: Pseudomonas sp. and Bacillus sp.; consortium B: Pseudomonas sp. and Bacillus sp.) from the Atacama Desert (northern Chile) and Antarctica, and their influence on physiological traits of Chenopodium quinoa growing in metal(loid)-contaminated soils. The metal(loid) sorption capacity of the consortia was determined. Bacteria were encapsulated using ionic gelation and were inoculated in soil of C. quinoa. The morphological variables, photosynthetic pigments, and lipid peroxidation in plants were evaluated. Consortium A showed a significantly higher biosorption capacity than consortium B, especially for As and Cu. The highest viability of consortia was achieved with matrices A1 (3% alginate and 2% bentonite) and A3 (3% alginate, 2% bentonite and 2.5% LB medium) at a drying temperature of 25 °C and storage at 4 °C. After 12 months, the highest viability was detected using matrix A1 with a concentration of 106 CFU g-1. Further, a greenhouse experiment using these consortia in C. quinoa plants showed that, 90 days after inoculation, the morphological traits of both consortia improved. Chemical analysis of metal(loid) contents in the leaves indicated that consortium B reduced the absorption of Cu to 32.1 mg kg-1 and that of Mn to 171.9 mg kg-1. Encapsulation resulted in a significant increase in bacterial survival. This highlights the benefits of using encapsulated microbial consortia from extreme environments, stimulating the growth of C. quinoa, especially in soils with metal(loid) levels that can be a serious constraint for plant growth.
期刊介绍:
Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.