藻酸盐-膨润土包裹极端嗜水细菌群能增强藜麦对金属应力的耐受性

IF 4.1 2区 生物学 Q2 MICROBIOLOGY
Cesar Arriagada-Escamilla, Roxana Alvarado, Javier Ortiz, Reinaldo Campos-Vargas, Pablo Cornejo
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引用次数: 0

摘要

本研究探讨了将两种来自阿塔卡马沙漠(智利北部)和南极洲的抗金属(loid)细菌联合体(联合体 A:假单胞菌和芽孢杆菌;联合体 B:假单胞菌和芽孢杆菌)封装在海藻酸盐/膨润土珠中,以及它们对生长在金属(loid)污染土壤中的藜麦生理特性的影响。对联合菌群的金属(loid)吸附能力进行了测定。利用离子凝胶法封装细菌并将其接种到藜麦土壤中。对植物的形态变量、光合色素和脂质过氧化进行了评估。联合体 A 的生物吸附能力明显高于联合体 B,尤其是对砷和铜的生物吸附能力。在干燥温度为 25 °C、储存温度为 4 °C、基质为 A1(3%海藻酸盐和 2%膨润土)和 A3(3%海藻酸盐、2%膨润土和 2.5% LB 培养基)时,联合体的存活率最高。12 个月后,基质 A1 的存活率最高,达到 106 CFU g-1。此外,在藜麦植株中使用这些菌群进行的温室实验表明,接种 90 天后,两种菌群的形态特征都有所改善。叶片中金属(loid)含量的化学分析表明,联合菌群 B 将铜的吸收量降至 32.1 毫克/千克,将锰的吸收量降至 171.9 毫克/千克。封装使细菌存活率显著提高。这凸显了使用来自极端环境的封装微生物菌群的益处,可刺激藜麦的生长,尤其是在金属(loid)含量严重制约植物生长的土壤中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alginate-Bentonite Encapsulation of Extremophillic Bacterial Consortia Enhances Chenopodium quinoa Tolerance to Metal Stress.

This study explores the encapsulation in alginate/bentonite beads of two metal(loid)-resistant bacterial consortia (consortium A: Pseudomonas sp. and Bacillus sp.; consortium B: Pseudomonas sp. and Bacillus sp.) from the Atacama Desert (northern Chile) and Antarctica, and their influence on physiological traits of Chenopodium quinoa growing in metal(loid)-contaminated soils. The metal(loid) sorption capacity of the consortia was determined. Bacteria were encapsulated using ionic gelation and were inoculated in soil of C. quinoa. The morphological variables, photosynthetic pigments, and lipid peroxidation in plants were evaluated. Consortium A showed a significantly higher biosorption capacity than consortium B, especially for As and Cu. The highest viability of consortia was achieved with matrices A1 (3% alginate and 2% bentonite) and A3 (3% alginate, 2% bentonite and 2.5% LB medium) at a drying temperature of 25 °C and storage at 4 °C. After 12 months, the highest viability was detected using matrix A1 with a concentration of 106 CFU g-1. Further, a greenhouse experiment using these consortia in C. quinoa plants showed that, 90 days after inoculation, the morphological traits of both consortia improved. Chemical analysis of metal(loid) contents in the leaves indicated that consortium B reduced the absorption of Cu to 32.1 mg kg-1 and that of Mn to 171.9 mg kg-1. Encapsulation resulted in a significant increase in bacterial survival. This highlights the benefits of using encapsulated microbial consortia from extreme environments, stimulating the growth of C. quinoa, especially in soils with metal(loid) levels that can be a serious constraint for plant growth.

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来源期刊
Microorganisms
Microorganisms Medicine-Microbiology (medical)
CiteScore
7.40
自引率
6.70%
发文量
2168
审稿时长
20.03 days
期刊介绍: Microorganisms (ISSN 2076-2607) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to prokaryotic and eukaryotic microorganisms, viruses and prions. It publishes reviews, research papers and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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