硫醇-SAM 浓度对基于插接电极的无氧化还原生物传感器性能的影响

IF 3 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Micromachines Pub Date : 2024-10-12 DOI:10.3390/mi15101254
Abdulaziz K Assaifan
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引用次数: 0

摘要

尽管非法拉第阻抗生物传感器具有直接、无氧化还原反应和检测简单的特点,但要提高其检测各种生物标记物的性能,还需要进行大量的优化工作。无雷达 EIS 传感器的性能取决于极化生物传感器表面与被测样品溶液之间的界面电容。我们鼓励对界面电容进行仔细的工程设计和设计,以便在生物受体与抗原相互作用时放大红外信号。实现这一目标的方法之一是优化自组装单层的浓度,目前还没有关于非法拉第阻抗传感器的报道。本文报告了烷硫酸盐(半胱胺)浓度对金(Au)交错电极(Au-IDE)生物传感器性能的影响。我们制备了六组生物传感器,每组的半胱胺浓度各不相同:100 nM、1 μM、10 μM、100 μM、1 mM 和 10 mM。通过戊二醛交联剂将低密度脂蛋白抗体附着在半胱胺上,制备出直接检测低密度脂蛋白胆固醇的生物传感器。随着半胱胺浓度从 100 nM 增加到 100 μM,生物传感器的灵敏度从 6.7 nF/ln (纳克/毫升)增加到 16.2 nF/ln(纳克/毫升)。随着半胱胺浓度从 100 μM 增加到 10 mM,灵敏度有所下降。当半胱胺浓度从 100 nM 增加到 100 μM(即从 400 ng/mL 增加到 59 pg/mL)时,生物传感器的检测限(LoD)有所提高。然而,半胱胺浓度为 1 mM 和 10 mM 时,LoD 开始分别增加到 67 pg/mL 和 16 ng/mL。这表明,半胱胺浓度会对无氧化还原生物传感器产生不利影响。半胱胺层必须尽可能薄,并均匀地覆盖电极表面,以最大限度地增加正读出信号,减少负读出信号,从而显著提高灵敏度和 LoD。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Thiol-SAM Concentration Effect on the Performance of Interdigitated Electrode-Based Redox-Free Biosensors.

Despite the direct, redox-free and simple detection non-faradaic impedimetric biosensors offer, considerable optimizations are required to enhance their performance for the detection of various biomarkers. Non-faradaic EIS sensors' performance depends on the interfacial capacitance between a polarized biosensor surface and the tested sample solution. Careful engineering and design of the interfacial capacitance is encouraged to magnify the redout signal upon bioreceptor-antigen interactions. One of the methods to achieve this goal is by optimizing the self-assembled monolayer concentration, which has not been reported for non-faradaic impedimetric sensors. Here, the impact of alkanethiolate (cysteamine) concentration on the performance of gold (Au) interdigitated electrode (Au-IDE) biosensors is reported. Six sets of biosensors were prepared, each with a different cysteamine concentration: 100 nM, 1 μM, 10 μM, 100 μM, 1 mM, and 10 mM. The biosensors were prepared for the direct detection of LDL cholesterol by attaching LDL antibodies on top of the cysteamine via a glutaraldehyde cross-linker. As the concentration of cysteamine increased from 100 nM to 100 μM, the sensitivity of the biosensor increased from 6.7 to 16.2 nF/ln (ng/mL). As the cysteamine concentration increased from 100 μM to 10 mM, the sensitivity deteriorated. The limit of detection (LoD) of the biosensor improved as the cysteamine increased from 100 nM to 100 μM (i.e., 400 ng/mL to 59 pg/mL). However, the LoD started to increase to 67 pg/mL and 16 ng/mL for 1 mM and 10 mM cysteamine concentrations, respectively. This shows that the cysteamine concentration has a detrimental effect on redox-free biosensors. The cysteamine layer has to be as thin as possible and uniformly cover the electrode surfaces to maximize positive readout signals and reduce negative signals, significantly improving both sensitivity and LoD.

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来源期刊
Micromachines
Micromachines NANOSCIENCE & NANOTECHNOLOGY-INSTRUMENTS & INSTRUMENTATION
CiteScore
5.20
自引率
14.70%
发文量
1862
审稿时长
16.31 days
期刊介绍: Micromachines (ISSN 2072-666X) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to micro-scaled machines and micromachinery. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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