在毛细管微反应器中使用莫比莱氏酵母菌和原位萃取法生产乙醇

IF 3 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Micromachines Pub Date : 2024-10-13 DOI:10.3390/mi15101255
Julia Surkamp, Lennart Wellmann, Stephan Lütz, Katrin Rosenthal, Norbert Kockmann
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引用次数: 0

摘要

本研究将莫比莱氏胸腺单胞菌(Zymomonas mobilis)作为一种模式生物进行研究,以便在连续流微反应器中通过原位萃取法培养和分离乙醇产品。所考虑的微反应器是盘流逆变器(CFI),它由盘绕在支撑结构上的毛细管组成。与其他微反应器一样,CFI 也得益于较高的表面体积比,从而加强了传质和传热。与许多其他微反应器相比,CFI 的优势在于无需内部结构,而内部结构通常用于确保良好的混合。CFI 设计简单,细胞不会被内部结构卡住或损坏,因此特别适合生化应用。尽管设计简单,但通过泰勒旋涡和迪恩旋涡产生的流动旋涡仍可实现良好的混合。反应系统由两相组成,其中水相携带细菌,油醇相用于提取产生的乙醇。评估的关键参数是细菌的生长和微生物产生的乙醇量。结果表明,CFI 适合微生物生产有价值的化合物。在 CFI 实验中,乙醇的最大浓度为 1.26 g L-1。总体而言,在 CFI 中培养能加快莫比莱氏菌的生长速度,使乙醇产量比间歇实验高出 25%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ethanol Production Using Zymomonas mobilis and In Situ Extraction in a Capillary Microreactor.

The bacterium Zymomonas mobilis is investigated as a model organism for the cultivation and separation of ethanol as a product by in situ extraction in continuous flow microreactors. The considered microreactor is the Coiled Flow Inverter (CFI), which consists of a capillary coiled onto a support structure. Like other microreactors, the CFI benefits from a high surface-to-volume ratio, which enhances mass and heat transfer. Compared to many other microreactors, the CFI offers the advantage of operating without internal structures, which are often used to ensure good mixing. The simplicity of the design makes the CFI particularly suitable for biochemical applications as cells do not get stuck or damaged by internal structures. Despite this simplicity, good mixing is achieved through flow vortices caused by Taylor and Dean vortices. The reaction system consists of two phases, in which the aqueous phase carries the bacterium and an oleyl alcohol phase is used to extract the ethanol produced. Key parameters for evaluation are bacteria growth and the amount of ethanol produced by the microorganism. The results show the suitability of the CFI for microbial production of valuable compounds. A maximum ethanol concentration of 1.26 g L-1 was achieved for the experiment in the CFI. Overall, the cultivation in the CFI led to faster growth of Z. mobilis, resulting in 25% higher ethanol production than in conducted batch experiments.

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来源期刊
Micromachines
Micromachines NANOSCIENCE & NANOTECHNOLOGY-INSTRUMENTS & INSTRUMENTATION
CiteScore
5.20
自引率
14.70%
发文量
1862
审稿时长
16.31 days
期刊介绍: Micromachines (ISSN 2072-666X) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to micro-scaled machines and micromachinery. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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